Xinxin Hu1,2,#, Yuye Xue3,#, Fei Fang4, Jie Li2, Xiaofeng Yuan2, Guang Cheng5, Hailong Yuan3, Yongqiang Zhang2, Yuefei Zhou5, Shuangwu Yang5, Pengcheng Qiu2,*, Yunyang Lu2, Haifeng Tang2,*
Oncology Research, Vol.33, No.9, pp. 2491-2506, 2025, DOI:10.32604/or.2025.064454
- 28 August 2025
Abstract Objectives: The Sorbin and SH3 domain containing 1 (SORBS1), a protein linked to insulin signaling CBL interaction, was investigated for its role in pancreatic cancer apoptosis. This study explored polyphyllin H (PPH)’s ability to restore SORBS1-knockdown-mediated repair functions. Methods: PANC-1 cells were divided into Blank, overexpression (OE), and knockdown groups. CCK-8 assays assessed proliferation and drug toxicity. Western blot and flow cytometry analyzed SORBS1 levels and PPH effects. Comet assays quantified DNA damage. Subcutaneous xenograft tumors in nude mice (Blank vs. knockdown) were treated with PPH to evaluate in vivo efficacy. SORBS1-H2AX gene correlation was analyzed… More >
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