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    Physiological and biochemical changes during organogenesis and somatic embryogenesis of HBsAg-transgenic cherry tomato mutant

    Guan Z-J1,3,4*, S-B Lu2*,Y-L Huo5, B Guo3, Z-P Guan6, Y-H Wei3

    Phyton-International Journal of Experimental Botany, Vol.84, No.2, pp. 351-357, 2015, DOI:10.32604/phyton.2015.84.351

    Abstract Leaf explants of the HBsAg-transgenic cherry tomato (Solanum lycopersicum) mutant were cultured on Murashige and Skoog (MS) basal medium, supplemented with 1.0 mg/L 6-BA and 0.05 mg/L IAA for callus induction, to clarify the physiological and biochemical characteristics of morphogenesis development. Therefore, the physiological and biochemical changes during the development of organogenic shoots and somatic embryos in the mutant were studied. Superoxide dismutase (SOD) activities of the mutant had only one peak value on the 21st day. Peroxidase (POD) activities of the mutant declined less sharply since the explants were cultured. IAA oxidase activity of the mutant increased steadily until… More >

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