Wentao Sheng, Xi Lei, Xinjie Chen, Quan Kuang^*

Phyton-International Journal of Experimental Botany, Vol.93, No.8, pp. 1897-1911, 2024, DOI:10.32604/phyton.2024.052395 - 30 August 2024

Abstract The analysis of chloroplast gene characteristics in Alpinia japonica (Thunb.) Miq. is of great significance for developing relevant genetic resources. The high-throughput sequencing and bioinformatic research were performed to analyze the chloroplast genome characteristics of A. japonica. The total chloroplast genome length of A. japonica was 161,906 bp, with a typical circular tetrameric structure. And 133 genes were annotated, comprising 87 protein-coding, 38 tRNA, and 8 rRNA genes. Furthermore, 22 genes contained two copies, and 18 genes owned introns. Repeat sequence analysis showed that it contains 321 simple sequence repeats (SSRs) and 37 long segment repeats. Compared with… More >

Chaoying Wang¹, Lihui Ma², Yingzan Xie², Bo Ding³, Jingsi Huang⁴, Qian Wang^4,*

Phyton-International Journal of Experimental Botany, Vol.93, No.8, pp. 1981-1993, 2024, DOI:10.32604/phyton.2024.050099 - 30 August 2024

Abstract Myricaria laxiflora, which grows along the Yangtze River in China, holds ornamental, ecological, and medicinal value. However, its wild population is threatened and currently designated protected as a national priority. The present research was the first to sequence and assemble M. laxiflora’s mitochondrial genome and examine its structural characteristics and phylogenetic relationships with other sequenced Caryophyllales species. The mitochondrial double-stranded closed-ring genome of M. laxiflora was found to be 389,949 bp in length, containing numerous repetitive sequences and RNA editing sites, with 34 protein encoding, 21 tRNA, and 3 rRNA genes. Although there are 22 fragments in the More >

Riwa Mahai¹, Rongpeng Liu¹, Xiaolang Du¹, Zejing Mu¹, Xiaoyun Wang^1,*, Jun Yuan^2,*

Phyton-International Journal of Experimental Botany, Vol.93, No.6, pp. 1203-1219, 2024, DOI:10.32604/phyton.2024.051559 - 27 June 2024

Abstract Rosaceae represents a vast and complex group of species, with its classification being intricate and contentious. The taxonomic placement of many species within this family has been a subject of ongoing debate. The study utilized the Illumina platform to sequence 19 plant species from 10 genera in the Rosaceae. The cp genomes, varying in size from 153,366 to 159,895 bp, followed the typical quadripartite organization consisting of a large single-copy (LSC) region (84,545 to 87,883 bp), a small single-copy (SSC) region (18,174 to 19,259 bp), and a pair of inverted repeat (IR) regions (25,310 to… More >

Zhengfeng Lai, Dongmei Lian, Shaoping Zhang, Yudong Ju, Bizhen Lin, Yunfa Yao, Songhai Wu, Jianji Hong, Zhou Li^*

Phyton-International Journal of Experimental Botany, Vol.93, No.6, pp. 1143-1158, 2024, DOI:10.32604/phyton.2024.051207 - 27 June 2024

Abstract The cytokinin oxidase/dehydrogenase (CKX) enzyme is essential for controlling the fluctuating levels of endogenous cytokinin (CK) and has a significant impact on different aspects of plant growth and development. Nonetheless, there is limited knowledge about CKX genes in tomato (Solanum lycopersicum L.). Here we performed genome-wide identification and analysis of nine SlCKX family members in tomatoes using bioinformatics tools. The results revealed that nine SlCKX genes were unevenly distributed on five chromosomes (Chr.1, Chr.4, Chr.8, Chr.10, and Chr.12). The amino acid length, isoelectric points, and molecular weight of the nine SlCKX proteins ranged from 453 to 553, 5.77… More >

Adel A. Rezk^1,2,*, Hala A. Amin²

Phyton-International Journal of Experimental Botany, Vol.92, No.3, pp. 691-705, 2023, DOI:10.32604/phyton.2022.024208 - 29 November 2022

Abstract Citrus Tristeza Virus (CTV), usually occurs in nature as a mixture of genotypes. Six naturally infected citrus (Citrus sinensis) trees grafted on sour orange rootstock were collected from three citrus growing governorates in Egypt (Sharqia, Qalyubia and Garbia). In this study, RT-PCR, Single-Strand Conformation Polymorphism (SSCP) and nucleotide sequence analysis were used for four independent CTV genomic regions (p65, p18, p20, and p23) to detect and assess the sequence and genetic variabilities among CTV Egyptian isolates. RTPCR products (650 bp) for the CTV p23 gene obtained from the selected isolates were used for the SSCP analysis… More >

Xiaolei Ding, Ruiwen Zhao, Sixi Lin, Jianren Ye^*

Phyton-International Journal of Experimental Botany, Vol.91, No.12, pp. 2813-2825, 2022, DOI:10.32604/phyton.2022.024609 - 29 August 2022

Abstract In Bazhong City, Sichuan Province, China, top dieback symptoms were found on many pine trees (Pinus thunbergii Parl). The tips of old needles first turned grayish-green and then developed into brown bands in the field. Phylogenetic analysis of concatenated ITS and EF1-α indicated the pathogen of this dieback disease as Diplodia neojuniperi. Additionally, effects of temperature, pH and medium on the mycelial growth were also characterized. The most favorable temperature and pH level for mycelial growth are 25°C and 8, respectively. The optimal medium for mycelial growth is PDA medium. To our knowledge, this is the first More >

Zhiguo Lu¹, Yuhong Zheng^1,*, Pengchong Zhang², Boyuan Fan³, Aimin Yu⁴, Li Fu^3,*

Phyton-International Journal of Experimental Botany, Vol.91, No.11, pp. 2549-2558, 2022, DOI:10.32604/phyton.2022.021288 - 12 July 2022

Abstract In this communication, we used electrochemical sensor for recording the electrochemical profiles of eleven species of Yulania spp. from leaf extract. Two solvents and two buffer conditions were used for electrochemical fingerprints collection. Their electrochemical fingerprints can be converted to different patterns and consequently for species recognition. The results indicate the pattern recognition is much convenient than that of the recognition of species directly using voltammetric signal. The current information in electrochemical fingerprinting represents the type and amount of electrochemically active molecules, which linked to the genetic differences among the plants. Therefore, the electrochemical fingerprints were More >

ZHENGGANG XU^1,2,3, LIANG WU³, JIAHAO CHEN¹, YUNLIN ZHAO³, CHONGXUAN HAN¹, TIAN HUANG², GUIYAN YANG^1,*

BIOCELL, Vol.46, No.7, pp. 1733-1746, 2022, DOI:10.32604/biocell.2022.015784 - 17 March 2022

Abstract Darwin’s finches are the most classic case of evolution. Early studies on the evolution of this species were mainly based on morphology. Until now, the mitochondrial genome of Geospiza magnirostris has been sequenced and the study explored the characteristics of the complete genome of G. magnirostris and verified the evolutionary position of it. The 13 PCGs initiated by ATN codons. The stop codons of three PCGs (ND2, COX3 and ND4) were incomplete, with only T- or TA- replacing complete form TAA or TAG. All the tRNA genes expressed a typical cloverleaf secondary structure, except for tRNA^Ser1(AGY), whose dihydrouridine (DHU)… More >

HUI-YING JIN^1,2, MAO-SONG PEI^1,2, DA-LONG GUO^1,2,*

BIOCELL, Vol.46, No.4, pp. 941-949, 2022, DOI:10.32604/biocell.2022.016718 - 15 December 2021

Abstract To understand the molecular characteristics of the miR477 gene family of grape (Vvi-miR477) and to predict its target genes, the Vvi-miR477 genes were identified from previous small RNA sequencing data, then phylogenetic analysis and prediction of target gene were conducted. The Vvi-miR477 family consists of two precursor sequences and three mature sequences. The miR477 family members were mostly 19-22nt in length. The sequence is relatively conservative. Vvi-MIR477a and Vvi-MIR477b are located on chromosomes 1 and 2, respectively. These precursor sequences can form the typical stable stem-loop structure. Their minimum folding free energy is −39.10 kcal/mol and −50.90 kcal/mol, More >

Yizhao Huang¹, Nannan Li², Deya Wang⁴, Jian Du¹, Weitang Liu³, Jinxin Wang^3,*, Wei Li^1,*

Phyton-International Journal of Experimental Botany, Vol.91, No.4, pp. 761-772, 2022, DOI:10.32604/phyton.2022.018462 - 09 December 2021

Abstract Beckmannia syzigachne is a noxious weed for rice-wheat rotations in China. The B. syzigachne (AH-02) population evolved metabolic resistance to fenoxaprop-P-ethyl and mesosulfuron-methyl. To investigate the function of GT73C1 in this population, the GT73C1 gene was amplified by reverse transcription-polymerase chain reaction, and the sequence was 100% consistent with the transcriptome data. Its phylogenetic tree was displayed and annotated using FigTree v1.4.4. The plant overexpression vector of GT73C1 gene was constructed and used to transform Brachypodium distachyon plants. Furthermore, the expression of GT73C1 was significantly induced by fenoxaprop-P-ethyl and mesosulfuron-methyl, which was consistent with the findings from the whole plant More >