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  • Open Access

    ARTICLE

    Bronchoalveolar lavage fluid metagenomic next-generation sequencing assay for identifying pathogens in lung cancer patients

    JIYU WANG1,2, HUIXIA LI1,2, DEYUAN ZHOU1,2, LIHONG BAI1,2, KEJING TANG1,2,3,*

    BIOCELL, Vol.48, No.4, pp. 623-637, 2024, DOI:10.32604/biocell.2024.030420

    Abstract Background: For patients with lung cancer, timely identification of new lung lesions as infectious or non-infectious, and accurate identification of pathogens is very important in improving OS of patients. As a new auxiliary examination, metagenomic next-generation sequencing (mNGS) is believed to be more accurate in diagnosing infectious diseases in patients without underlying diseases, compared with conventional microbial tests (CMTs). We designed this study to find out whether mNGS has better performance in distinguishing infectious and non-infectious diseases in lung cancer patients using bronchoalveolar lavage fluid (BALF). Materials and Methods: This study was a real-world retrospective review based on electronic medical… More >

  • Open Access

    REVIEW

    Review on microbial metabolomics of probiotics and pathogens: Methodologies and applications

    XIN MENG*, XUE LI, LIANRONG YANG, RUI YIN, LEHUI QI, QI GUO

    BIOCELL, Vol.47, No.1, pp. 91-107, 2023, DOI:10.32604/biocell.2023.024310

    Abstract In recent years, microbial metabolomics, a new field that has attracted wide attention, provides a map of metabolic pathways and clarifies the interaction mechanism between microorganisms and hosts. Many microorganisms are found in the human intestine, oral cavity, vagina, etc. Probiotics could maintain the good health of the host, while pathogens and an imbalance of bacterial flora lead to a series of diseases of the body and mind. Metabolomics is a science for qualitative and quantitative analysis of all metabolites in an organism or biological system, which could provide key information to understand the related metabolic pathways and associated changes.… More >

  • Open Access

    ARTICLE

    In Vitro Anti-Bacterial and Anti-Fungal Activities of Extracts from Different Parts of 7 Zingiberaceae Plants

    Lixian Wu1,#, Yongquan Li1,#, Qiaoguang Li2, Jingxuan Li1, Qinghua Lao1, Yucheng Zhong1, Hui Zhang1,*, Xiu Hu1,*

    Journal of Renewable Materials, Vol.11, No.2, pp. 975-989, 2023, DOI:10.32604/jrm.2022.023547

    Abstract This study aimed to explore the anti-bacterial and anti-fungal activities of extracts from different parts of plants in the Zingiberaceae family. The inhibitory rate, minimum inhibitory concentration (MIC), and minimum bactericidal concentration (MBC) of leaf and stem, and root and rhizome extracts from Alpinia katsumadai Hayata, Alpinia oxyphylla Miq × Alpinia henryi K. Schumann, Alpinia oblongifolia Hayata, Alpinia nigra (Gaertn.) Burtt, Amomum villosum Lour, Alpinia zerumbet (Pers.) Burtt. et Smith and Alpinia oxyphylla Miq were determined using the fungus cake method and double dilution method. The seven Zingiberaceae plants exhibited characteristic antibacterial activities against pathogenic bacteria and fungi. At a… More >

  • Open Access

    ARTICLE

    Synthesis, Characterization and Remedial Action of Biogenic Silver Nanoparticles and Chitosan-Silver Nanoparticles against Bacterial Pathogens

    Piyush Kumar Gupta1, D. Karthik Kumar2, M. Thaveena3, Soumya Pandit1, Somya Sinha4, R. Ranjithkumar2,* , Walaa F. Alsanie5, Vijay Kumar Thakur6,7,8,*

    Journal of Renewable Materials, Vol.10, No.12, pp. 3093-3105, 2022, DOI:10.32604/jrm.2022.019335

    Abstract Custard apple is a dry land fruit. Its leaves exhibit different pharmacological activities. In the present study, both silver (Ag) nanoparticles and chitosan-coated Ag (Chi-Ag) nanoparticles were fabricated using the aqueous leaf extract of the custard apple plant. During preliminary phytochemical analysis, various types of phytocompounds were found in the aqueous leaf extract of the same plant. Next, both nanoparticles were physiochemically characterized. FTIR analysis exhibited the fingerprint vibrational peaks of active bioactive compounds in plant extract, Ag nanoparticles, and Chi-Ag nanoparticles. UV/Visible spectral analysis revealed the highest absorbance peak at 419 nm, indicating the presence of Ag nanoparticles. XRD… More > Graphic Abstract

    Synthesis, Characterization and Remedial Action of Biogenic Silver Nanoparticles and Chitosan-Silver Nanoparticles against Bacterial Pathogens

  • Open Access

    REVIEW

    Antagonistic Potential of Bacterial Species against Fungal Plant Pathogens (FPP) and Their Role in Plant Growth Promotion (PGP): A Review

    Amjad Ali1, Yasir Iftikhar2,*, Mustansar Mubeen2, Haider Ali3, Muhammad Ahmad Zeshan2, Zohaib Asad4, Muhammad Zafar-ul-Hye5, Malik Abdul Rehman6, Mazhar Abbas7, Muhammad Rafique8, Muhammad Usman Ghani9

    Phyton-International Journal of Experimental Botany, Vol.91, No.9, pp. 1859-1877, 2022, DOI:10.32604/phyton.2022.021734

    Abstract

    Since the 19th century to date, the fungal pathogens have been involved in causing devastating diseases in plants. All types of fungal pathogens have been observed in important agricultural crops that lead to significant pre and postharvest losses. The application of synthetic fungicide against the fungal plant pathogens (FPP) is a traditional management practice but at the same time these fungicides kill other beneficial microbes, insects, animal, and humans and are harmful to environment. The antagonistic microorganism such as bacteria are being used as an alternate strategy to control the FPP. These antagonistic species are cost-effective and eco-friendly in nature.… More > Graphic Abstract

    Antagonistic Potential of Bacterial Species against Fungal Plant Pathogens (FPP) and Their Role in Plant Growth Promotion (PGP): A Review

  • Open Access

    ARTICLE

    Antimicrobial activities of three seaweeds extract against some human viral and bacterial pathogens

    JELAN MOFEED1,2, MOHAMED DEYAB3, ABDELNASER MOHAMED3, MAHMOUD MOUSTAFA4,5, SALLY NEGM6,7, EMAD EL-BILAWY1

    BIOCELL, Vol.46, No.1, pp. 247-261, 2022, DOI:10.32604/biocell.2022.015966

    Abstract

    Microbial infections cause complicated health influences along with bad economic impacts. In the present investigation, three dominant seaweeds namely, Amphiroa anceps, Corallina officinalis and Sargassum filipendula were collected from different Egyptian sites at the Red Sea and Mediterranean Sea during autumn 2019. Organic extracts of the three algae were screened for their antibacterial activity against three pathogenic bacteria Salmonella typhiimurium, Staphylococcus aureus and Escherichia coli, in addition to in vitro antiviral activity against Rotavirus (RV), and Coxsackie virus B3 (CVB3) that cause severe diseases in human. Organic extract of A. anceps, C. officinalis and S. filipendula inhibit E. coli cells… More >

  • Open Access

    ARTICLE

    Suppression Effects on Pineapple Soil-Borne Pathogens by Crotalaria juncea, Dolomitic Lime and Plastic Mulch Cover on MD-2 Hybrid Cultivar

    Luis Alfonso Aguilar Pérez1,*, Daniel Nieto Ángel1,*, Moisés Roberto Vallejo Pérez2, Daniel Leobardo Ochoa Martínez1, David Espinosa Victoria3, Andrés Rebolledo Martinez4, Abel Rebouças São José5

    Phyton-International Journal of Experimental Botany, Vol.90, No.4, pp. 1205-1216, 2021, DOI:10.32604/phyton.2021.015109

    Abstract The development and implementation of sustainable and environmentally friendly agricultural practices are indispensable as alternatives to pesticide use and to keep populations of soil-borne plant pathogens at levels that do not affect crop productivity. The present research evaluates the incidence of soil-borne phytopathogens on the pineapple variety MD-2, which was subjected to different treatments: Incorporation of Crotalaria juncea into the soil (organic amendment), application of dolomitic lime to soil (inorganic amendment), and the use of plastic mulch covering the soil. During the crop cycle (15 months), the following variables were evaluated: plant height (cm), fruit weight (kg·plant−1 ), crop yield… More >

  • Open Access

    ARTICLE

    Antimicrobial evaluation of extracts obtained from tropical micromycetes against phytopathogens

    Moreno Pérez P1,3, M Gamboa Angulo1, G Heredia2, B Canto Canché1, CM Rodríguez García1, IL Medina Baizabal1, L Peraza Echeverría1

    Phyton-International Journal of Experimental Botany, Vol.85, pp. 7-14, 2016, DOI:10.32604/phyton.2016.85.007

    Abstract Nowadays, it is highly necessary to find more and safer agrochemicals. In this sense, micromycetes are an important source of natural products which could be used to control plant diseases. Therefore with the aims to contribute in this searching of natural products with antimicrobial applications, a total of 49 fungal strains were isolated from the Yucatan Peninsula. These fungi were cultured in fermented rice, their respective organic extracts macerated in ethyl acetate (EAE) and methanol (ME) were obtained and tested against five fungal pathogens of agricultural importance. These included Alternaria chrysanthemi, Colletotrichum gloeosporioides, Mycosphaerella fijiensis, and the bacteria Erwinia carotovoraMore >

  • Open Access

    ARTICLE

    In vitro antagonistic activity of Bacillus subtilis strains isolated from soils of the Yucatan Peninsula against Macrophomina phaseolina and Meloidogyne incognita

    Ruiz SE, AJ Cristóbal, RA Reyes, SJ Tun, RA García, AJ Pacheco

    Phyton-International Journal of Experimental Botany, Vol.83, pp. 45-47, 2014, DOI:10.32604/phyton.2014.83.045

    Abstract The antagonistic activity of native Bacillus subtilis strains from Yucatán peninsula soils were evaluated on two soilborne pathogens. Bacillus subtilis cbck36 and cbrf24 caused more than 60% inhibition of colony growth in Machophomina phaseolina. Cell-free culture filtrate of B. subtilis cbr24 were active against second- stage juveniles (J2) of Meloidogyne incognita (LC50 25.8% v/v). More >

  • Open Access

    ARTICLE

    In vitro biocontrol of tomato pathogens using antagonists isolated from chicken-manure vermicompost

    Barocio-Ceja NB1, LF Ceja-Torres1*, JL Morales-García2, HV Silva-Rojas3, R Flores-Magallón1, S Ochoa-Estradav1

    Phyton-International Journal of Experimental Botany, Vol.82, pp. 15-22, 2013, DOI:10.32604/phyton.2013.82.015

    Abstract The objectives of this study were to (1) isolate and identify pathogenic fungi from vegetative material with wilt symptoms in tomato plantations belonging to the Cienega of Chapala, Michoacán, Mexico, and (2) determine the antagonistic capacity of Trichoderma sp. and Aspergillus spp. isolated from chicken-manure vermicompost. Pathogens were isolated by means of a completely randomized sampling in 6 locations; 9 plantations were inspected and 45 plants with symptoms of the disease were selected. Portions of root and stem were disinfected and placed on potato-dextroseagar acidified (PDA). Antagonists isolation was made from a dilution of chicken-manure vermicompost of 1 x 10-2More >

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