ZHIHUI WANG1, YE YUAN2,*
BIOCELL, Vol.44, No.4, pp. 731-736, 2020, DOI:10.32604/biocell.2020.010575
- 24 December 2020
Abstract Glyphosate is a non-selective broad-spectrum herbicide that blocks plant growth by inhibiting 5-
Enolpyruvylshikimate-3-phosphate synthase (EPSPS), a key enzyme of the shikimate pathway in microorganisms and
plants. The full-length epsps cDNA sequence (paepsps, Genebank: KY860582.1) was cloned and characterized for the
first time from Phragmites australis. The full-length cDNA of paepsps was 1308 bp encoding a polypeptide of 435
amino acids. The bioinformatic analyses showed that PaEPSPS has highly homologous with EPSPS from other plants.
RT-PCR analysis of paepsps expression indicated that the gene expressed in leaves, stems, and roots, with higher
expression in leaves. The expression of More >
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