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  • Open Access

    ARTICLE

    Valorisation of Northern Moroccan Centaurium erythraea: Targeted Phytochemistry, Antioxidant, Antimicrobial Efficacy and Drug Likeness Benchmarking

    Yousra Hammouti1,2,*, François Mesnard2, Oussama Khibech3, Mohamed Taibi1, Yousra Belbachir3, El Hassania Loukili4, Reda Bellaouchi5, Abdeslam Asehraou5, Mohamed Addi1,*

    Phyton-International Journal of Experimental Botany, Vol.94, No.11, pp. 3563-3583, 2025, DOI:10.32604/phyton.2025.071139 - 01 December 2025

    Abstract Centaurium erythraea Rafn (“Gosset El Haya”) has long been prized in North African folk medicine, yet Moroccan chemobiological data remain scarce. Ethanol extracts of northern Moroccan aerial parts were profiled by high-performance liquid chromatography (HPLC) and found rich in phenolics, dominated by 4-hydroxybenzoic acid (57.8%) and naringin (10.3%). The extract exhibited strong antioxidant power in the 2,2-diphenyl-1-picrylhydrazyl (DPPH) radical-scavenging assay, with a half-maximal inhibitory concentration (IC50) of ≈74 µg mL−1, and a total antioxidant capacity (TAC) of ≈201 µg mL−1 and selective antimicrobial activity, sharply inhibiting Aspergillus niger, Penicillium digitatum, and Rhodotorula glutinis while sparing Staphylococcus aureus. In-silico absorption, distribution, metabolism,… More >

  • Open Access

    ARTICLE

    HPLC-DAD Profiling and Diuretic Effect of Solanum elaeagnifolium (Cav.) Aqueous Extract: A Combined Experimental and Computational Approach

    Bouslamti Mohammed1, Nouioura Ghizlane1, Tbatou Widad1, Mohamed Chebaibi2,3, Abdulsalam Alhalmi4, Najoua Soulo1, Sulaiman Mohammed Alnasser5, Fahad M Alshabrmi6, Amine Elbouzidi7,*, Lyoussi Badiaa1, Benjelloun Ahmed Samir1

    Phyton-International Journal of Experimental Botany, Vol.94, No.5, pp. 1505-1518, 2025, DOI:10.32604/phyton.2025.063896 - 29 May 2025

    Abstract The Solanum genus is known for its diverse bioactive compounds, yet its diuretic potential remains understudied. This research commenced with an analysis of polyphenol and flavonoid content in Solanum elaeagnifolium leaf extract (SEFE) using colorimetric techniques, followed by HPLC-DAD to delineate its chemical composition. The aqueous extract revealed prominent constituents: naringin (12.38%), quercetin 3-O-B-D-Glucoside (27.25%), and flavone (15.26%). A 15-day study on normal rats investigated the diuretic potential of SEFE at repeated doses. SEFE significantly increased urine volume and urinary sodium/potassium levels without inducing hypokalaemia, contrasting with furosemide, a standard diuretic that induced hypokalaemia. Conversely, furosemide, a… More >

  • Open Access

    ARTICLE

    Identification of Secondary Metabolites of Lycium ruthenicum Murray by UPLC-QTOF/MS and Network Pharmacology of Its Anti-Inflammatory Properties

    Chen Chen#,*, Chunli Li#, Tengfei Li, Qianhong Li, Luyao Li, Fengqin Liu

    Phyton-International Journal of Experimental Botany, Vol.94, No.3, pp. 793-807, 2025, DOI:10.32604/phyton.2025.063549 - 31 March 2025

    Abstract Lycium ruthenicum Murray, a plant widely cultivated in northwestern China, is integral to traditional Chinese medicine, with applications in treating menstrual disorders, cardiovascular diseases, and menopausal symptoms. Despite its recognized medicinal value and use as a functional food, comprehensive knowledge of its metabolites and their pharmacological effects remains limited. This study presents an innovative approach using ultra-performance liquid chromatography coupled with quadrupole time-of-flight mass spectrometry (UPLC–QTOF/MS) to conduct a detailed analysis of both wild and cultivated L. ruthenicum samples. A total of 62 peaks were detected in the total ion current profile, with 59 metabolites identified based… More >

  • Open Access

    ARTICLE

    Towards Net Zero Resilience: A Futuristic Architectural Strategy for Cyber-Attack Defence in Industrial Control Systems (ICS) and Operational Technology (OT)

    Hariharan Ramachandran1,*, Richard Smith2, Kenny Awuson David1,*, Tawfik Al-Hadhrami3, Parag Acharya1

    CMC-Computers, Materials & Continua, Vol.82, No.2, pp. 3619-3641, 2025, DOI:10.32604/cmc.2024.054802 - 17 February 2025

    Abstract This paper introduces the Integrated Security Embedded Resilience Architecture (ISERA) as an advanced resilience mechanism for Industrial Control Systems (ICS) and Operational Technology (OT) environments. The ISERA framework integrates security by design principles, micro-segmentation, and Island Mode Operation (IMO) to enhance cyber resilience and ensure continuous, secure operations. The methodology deploys a Forward-Thinking Architecture Strategy (FTAS) algorithm, which utilises an industrial Intrusion Detection System (IDS) implemented with Python’s Network Intrusion Detection System (NIDS) library. The FTAS algorithm successfully identified and responded to cyber-attacks, ensuring minimal system disruption. ISERA has been validated through comprehensive testing scenarios… More >

  • Open Access

    ARTICLE

    Functional Studies of Castor (Ricinus communis L.) PLC Family Genes in Arabidopsis Inflorescence Development

    Yanpeng Wen1,#, Rui Luo1,#, Lili Li1, Xiaotian Liang1, Xuemei Hu1, Ruxin Li1, Zhiyan Wang1, Fenglan Huang1,2,3,4,5,*

    Phyton-International Journal of Experimental Botany, Vol.92, No.11, pp. 3091-3112, 2023, DOI:10.32604/phyton.2023.030960 - 24 October 2023

    Abstract Castor (Ricinus communis L.) is one of the top 10 oil crops in the world, and inflorescence is a trait that directly affects its yield. Phospholipase C (PLCs) is involved in many plant activities and metabolic processes. To study the functions of PLC family genes in the regulation of the inflorescence development of the female line of Lm-type castor aLmAB2, we determined the expression levels of six PLC family genes of three types of inflorescences of aLmAB2 (isofemale line, female line, bisexual line) at different developmental stages. The results showed that the 6 genes of the castor PLCMore >

  • Open Access

    ARTICLE

    Targeting LncRNA LLNLR-299G3.1 with antisense oligonucleotide inhibits malignancy of esophageal squamous cell carcinoma cells in vitro and in vivo

    LI TIAN1,#, YONGYI HUANG1,#, BAOZHEN ZHANG2,#, YI SONG1,#, LIN YANG3, QIANQIAN CHEN1, ZHENG WANG3, YILING WANG1, QIHAN HE1, WENHAN YANG1, SHUYONG YU4, TIANYU LU5, ZICHEN LIU1, KAIPING GAO1,*, XIUJUN FAN2,*, JIAN SONG4,*, RIHONG ZHAI1,*

    Oncology Research, Vol.31, No.4, pp. 463-479, 2023, DOI:10.32604/or.2023.028791 - 25 June 2023

    Abstract Accumulating evidence has indicated that long non-coding RNAs (lncRNAs) play critical roles in the development and progression of cancers, including esophageal squamous cell carcinoma (ESCC). However, the mechanisms of lncRNAs in ESCC are still incompletely understood and therapeutic attempts for in vivo targeting cancer-associated lncRNA remain a challenge. By RNA-sequencing analysis, we identified that LLNLR-299G3.1 was a novel ESCC-associated lncRNA. LLNLR-299G3.1 was up-regulated in ESCC tissues and cells and promoted ESCC cell proliferation and invasion. Silencing of LLNLR-299G3.1 with ASO (antisense oligonucleotide) resulted in opposite effects. Mechanistically, LLNLR-299G3.1 bound to cancer-associated RNA binding proteins and regulated the expression… More >

  • Open Access

    ARTICLE

    Real Time Automation and Ratio Control Using PLC & SCADA in Industry 4.0

    Basant Tomar*, Narendra Kumar, Mini Sreejeth

    Computer Systems Science and Engineering, Vol.45, No.2, pp. 1495-1516, 2023, DOI:10.32604/csse.2023.030635 - 03 November 2022

    Abstract Industrial Control Systems (ICS) and SCADA (Supervisory Control and Data Acquisition) systems play a critical role in the management and regulation of critical infrastructure. SCADA systems brings us closer to the real-time application world. All process and equipment control capability is typically provided by a Distributed Control System (DCS) in industries such as power stations, agricultural systems, chemical and water treatment plants. Instead of control through DCS, this paper proposes a SCADA and PLC (Programmable Logic Controller) system to control the ratio control division and the assembly line division inside the chemical plant. A specific… More >

  • Open Access

    ARTICLE

    A Rapid Determination of 30 Prohibited Pesticide Residues in Lycii Fructus by UPLC-MS/MS

    Kesheng Lin1,3,#, Zhengqi Wang2,#, Li Wang1,3, Jiawen Zhou1,3, Lijuan Han1,3, Jingjing Wen2, Tingxia Dong2, Ran Duan2,*, Ning Li1,3,*

    Phyton-International Journal of Experimental Botany, Vol.92, No.2, pp. 423-437, 2023, DOI:10.32604/phyton.2022.023763 - 12 October 2022

    Abstract Prohibited pesticide residues have become one of the main factors affecting the quality and safety of Lycii Fructus, However, rarely studies focus on the rapid determination of these residues. Here, a total of 30 kinds of prohibited pesticide residues were determined by ultra-performance liquid chromatography tandem triple quadrupole mass spectrometry (UPLC-MS/MS) in five different process ways. Pretreatment methods, chromatographic separation and detection conditions in mass spectrometry were all optimized accordingly. Among the five different pretreatment methods, the first and third solid phase extraction failed to provide high recoveries of sulfosulfuron compounds (both lower than 60%).… More >

  • Open Access

    ARTICLE

    In Vitro Anti-Bacterial and Anti-Fungal Activities of Extracts from Different Parts of 7 Zingiberaceae Plants

    Lixian Wu1,#, Yongquan Li1,#, Qiaoguang Li2, Jingxuan Li1, Qinghua Lao1, Yucheng Zhong1, Hui Zhang1,*, Xiu Hu1,*

    Journal of Renewable Materials, Vol.11, No.2, pp. 975-989, 2023, DOI:10.32604/jrm.2022.023547 - 22 September 2022

    Abstract This study aimed to explore the anti-bacterial and anti-fungal activities of extracts from different parts of plants in the Zingiberaceae family. The inhibitory rate, minimum inhibitory concentration (MIC), and minimum bactericidal concentration (MBC) of leaf and stem, and root and rhizome extracts from Alpinia katsumadai Hayata, Alpinia oxyphylla Miq × Alpinia henryi K. Schumann, Alpinia oblongifolia Hayata, Alpinia nigra (Gaertn.) Burtt, Amomum villosum Lour, Alpinia zerumbet (Pers.) Burtt. et Smith and Alpinia oxyphylla Miq were determined using the fungus cake method and double dilution method. The seven Zingiberaceae plants exhibited characteristic antibacterial activities against pathogenic bacteria and fungi. At a 1.5 mg mL−1, A. zerumbetMore >

  • Open Access

    ARTICLE

    Rapid Analysis of Four Alkaloids in Uncaria rhynchophylla by Core-Shell Column HPLC and Quantitative Analysis of Multi-Components by Single Marker (QAMS)

    Kesheng Lin1,2, Jiawen Zhou1,2, Lijuan Han1,2, Ning Li1,2,*

    Phyton-International Journal of Experimental Botany, Vol.92, No.1, pp. 285-296, 2023, DOI:10.32604/phyton.2022.023219 - 06 September 2022

    Abstract As a traditional herbal medicine, the major alkaloids in Uncaria rhynchophylla have been proven to have blood pressure-lowering and sedative effects. It is essential to develop an effective method for the determination of the major alkaloids in U. rhynchophylla. In this research, a rapid quantitative analysis involving multi-components analysis by a single marker strategy coupled with core-shell column HPLC was adopted to analyse four alkaloids (corynoxeine, isocorynoxeine, isorhynchophylline, rhynchophylline) in U. rhynchophylla. Isorhynchophylline was selected as the internal reference substance, the content of which was determined by the traditional external standard method. Relative correction factors (RCF) between isorhynchophylline More >

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