Jian Zhang*1, ZhenFeng Shi†1, JinXing Huang‡, XiaoGuang Zou§
Oncology Research, Vol.24, No.6, pp. 487-494, 2016, DOI:10.3727/096504016X14685034103752
Abstract This study aimed to investigate the pivotal role of cystatin B (CSTB) in the development of gastric cancer and
to explore its possible regulatory mechanism. Human gastric cancer SGC-7901 cells as a model in vitro were
transfected with plasmid PCDNA3.1-CSTB and siRNA-CSTB using Lipofectamine 2000. Quantitative realtime PCR (qRT-PCR) and Western blotting were performed to determine the relative expression of CSTB and
PI3K/Akt/mTOR pathway-related protein. Moreover, MTT assay, Transwell assay, and flow cytometry were
used to assess cell proliferation, migration, and apoptosis, respectively. The results showed that CSTB was
significantly downregulated in SGC-7901 cells compared… More >
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