Feng Zhang*, Hong Sun†, Sai Zhang†, Xin Yang‡, Guogang Zhang*, Tao Su†
Oncology Research, Vol.25, No.5, pp. 709-719, 2017, DOI:10.3727/096504016X14772331883976
Abstract PER3, a circadian clock gene, plays an important role in colorectal cancer, but its action and underlying mechanism in colorectal cancer stem-like cells (CSCs) remain unclear. In this study, the colorectal CSCs were
enriched in colorectal HCT-116 sphere-forming cells, expressing lower levels of stem cell markers CD133,
CD44, LGR5, and SOX2 compared with HCT-116 cells. A drug-resistant strain from HCT-116 was established.
Western blot and qRT-PCR analysis showed that PER3 was downregulated in colorectal CSCs and drug-resistant
HCT-116. Overexpression of PER3 could strengthen 5-FU-induced inhibitory effects on colorectal CSCs, but
knockdown of PER3 decreased its… More >
Login
Register
