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  • Open Access

    ARTICLE

    Cloning and Function Identification of a Phytoene Desaturase Gene from Eucommia ulmoides

    Jiali Wang1, Xiangmei Chen1, Xiaozhen Huang1, Yichen Zhao1,*, Degang Zhao1,2,*

    Phyton-International Journal of Experimental Botany, Vol.92, No.5, pp. 1377-1389, 2023, DOI:10.32604/phyton.2023.026830 - 09 March 2023

    Abstract The phytoene desaturase (PDS) encodes a crucial enzyme in the carotenoid biosynthesis pathway. Silencing or inhibiting PDS expression leads to the appearance of mottled, chlorosis, or albino leaves. In this study, the CDS sequence of EuPDS (Eucommia ulmoides Phytoene Desaturase) was first cloned and then PDS was silenced in Nicotiana benthamiana. Result showed the expression level of EuPDS in leaves was higher than that in the roots and stems. In N. benthamiana leaves, which were treated by Agrobacterium for 24 h, photo-bleaching was shown on the fresh leaves one week after injection and the transcript level of PDS was down-regulated during the More >

  • Open Access

    ARTICLE

    Conceptual Design of Glass/Renewable Natural Fibre-Reinforced Polymer Hybrid Composite Motorcycle Side Cover

    M. A. F. A. Wahab1, S. M. Sapuan1,2,*, M. M. Harussani1, M. Y. M. Zuhri1, A. A. Saleh3

    Journal of Renewable Materials, Vol.9, No.11, pp. 1973-1989, 2021, DOI:10.32604/jrm.2021.016221 - 04 June 2021

    Abstract This paper presents the development process relating to the conceptual design of glass/renewable natural fibrereinforced polymer hybrid composite motorcycle side cover. Motorcycle side cover is a component frequently made from plastic or steel that functions on covering the motorcycle parts, components and systems such as frame, battery, electrical systems and mechanical systems. Function Analysis Systems Techniques (FAST) is used to identify the functions of motorcycle side cover. The right-side cover of motorcycle model SYM E-Bonus 110 has been physically studied to identify the competitive benchmarking criteria. The functions and competitive benchmarking criteria are then compiled… More >

  • Open Access

    ARTICLE

    Genetic variability of nanche in Tabasco, Mexico, determined with RAPDs

    Martínez ME1, JM Lesher G2, G Castañón N2, E de la Cruz L1, C Zapata H2

    Phyton-International Journal of Experimental Botany, Vol.82, pp. 209-214, 2013, DOI:10.32604/phyton.2013.82.209

    Abstract Nanche (Byrsonima crassifolia (L.) H.B.K.) grows in the southeastern region of the Mexican republic under both wild and semi-cultivated forms. Despite the importance of the fruit of this tree, only one paper at the molecular level has been published in Mexico, and possibly in America, to date. For this reason, the purpose of this research was to study the genetic variability of nanche trees that grow under both wild and semi-cultivated conditions in the state of Tabasco, Mexico. Molecular determinations were carried out in the Laboratorio de Genómica of the División Académica de Ciencias Biológicas (DACBiológicas),… More >

  • Open Access

    ARTICLE

    Obtaining new germplasm in Cenchrus ciliaris L. through induced-mutation and in vitro selection

    López Colomba E1, A Prina2, S Griffa1, AN Ribotta1, E Carloni1, E Tommasino1, C Luna1, E Biderbost1, K Grunberg1

    Phyton-International Journal of Experimental Botany, Vol.80, pp. 59-64, 2011, DOI:10.32604/phyton.2011.80.059

    Abstract Cenchrus ciliaris L., a forage grass of wide distribution in the north-west of Argentina, is a tetraploid (4x = 36) and obligate apomictic species. One way of obtaining novel germplasm is by induced mutations. In this work, physical and chemical mutations are combined with in vitro selection procedures seeking for new germplasm, with emphasis on salinity and drought tolerance. Mature seeds of Cenchrus ciliaris L. cv Biloela were subjected to treatments with X rays (400 Gy) and ethyl methanesulphonate (EMS) water solution (5.5 mM for 24 h). To perform in vitro selection, after 7 days of EMS or X More >

  • Open Access

    ARTICLE

    Genetic variability in 22 sugarcane hybrid varieties (Saccharum spp. Hybrid)

    Alejandre Rosas1 JA, ME Galindo Tovar2, HE Lee Espinosa2, OG Alvarado Gómez3

    Phyton-International Journal of Experimental Botany, Vol.79, pp. 87-94, 2010, DOI:10.32604/phyton.2010.79.087

    Abstract Development of new sugarcane varieties allows to increase sucrose production and faces challenges as new pests and diseases, and soil fertility losses. The objective of this study was to contribute to expand molecular tools in selecting useful progenitors for hybridization programs. With this purpose, 14 RAPDs primers were pre-screened, and four of them which produced clearly resolved, reproducible and polymorphic fragment patterns were selected for genetic analysis of 22 sugarcane variety hybrids. These were used in the Estación de Hibridación at Tapachula, Chiapas, as progenitors in genetic improvement programs. Genetic distance analysis showed three main More >

  • Open Access

    ARTICLE

    RAPD-determined genetic diversity of Phytophtora cinnamomi Rands of aguacate, Michoacán, Mexico

    Ochoa-Fuentes YM1, E Cerna2, J Landeros2, O Vazquez1, V Olalde3, A Flores2

    Phyton-International Journal of Experimental Botany, Vol.78, pp. 25-30, 2009, DOI:10.32604/phyton.2009.78.025

    Abstract Forty two Mexican isolates of Phytophthora cinnamomi Rands were examined for genetic diversity by the random amplified polymorphic DNA assay. The amount of genetic variation was evaluated by polymerase chain reaction amplification with a set of twenty one random 10-mer primers. All amplifications revealed scorable polymorphisms among the isolates, and a total of 23 band positions were scored for the 21 primers tested. Genetic distances among the isolates were calculated, and cluster analysis was used to generate a dendogram which showed relationships between them. These results suggest that the geographic groups were strong. More >

  • Open Access

    ARTICLE

    Morphological and genetic characteristics allow the identification of a collection of garlic cultivars in the North-Central region of Mexico

    Azuara Hernández1 L, H Silos Espino1, C Perales Segovia1, JF Gómez Leyva2, AG Alpuche Solís3, LM Macias Valdez4

    Phyton-International Journal of Experimental Botany, Vol.77, pp. 81-91, 2008, DOI:10.32604/phyton.2008.77.081

    Abstract The objective of this study was to compare the yield and genetic relationships between two Perla garlic selections, obtained by individual selection of cloves, and commercial varieties and cultivars adapted to diverse regions of Mexico (which did not have a selection process). Varieties with fewer cloves showed higher yields. Coreano and California produced 20 ton/ha (fresh weight) and Perla selections between 17-19 ton/ha (fresh weight). Six primers of the series OPB were tested for genetic characterization and OPB-17 was selected. With the amplified DNA fragments, a bina-ry matrix was generated; afterwards, a dissimilarity matrix and More >

  • Open Access

    ABSTRACT

    PDSL and SDSL Parallel Visualization Algorithms for Large-scale Finite Element Analysis Data in Distributed Parallel Computing Environment

    Jin Yeon Cho1, Yun Hyuk Choi2, You Me Song3, Chang Sik Kim4

    The International Conference on Computational & Experimental Engineering and Sciences, Vol.4, No.3, pp. 151-158, 2007, DOI:10.3970/icces.2007.004.151

    Abstract In this work, PDSL(pre-detection sort last) and SDSL(strip-wise decomposition sort last) parallel visualization algorithms are proposed for efficient visualization of massive data generated from large-scale parallel finite element analysis through investigating the characteristics of distributed parallel finite element analysis procedure. The proposed parallel visualization algorithms are based on the sort last approach, and designed to be highly compatible with the characteristics of domain-wise computation in parallel finite element analysis. To investigate the performances of proposed algorithms, in-house software is developed by applying the binary tree network communication pattern along with the proposed sorting algorithms, and More >

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