RéMY GAUTHIER^1,*, HéLèNE FOLLET², ANA-MARIA TRUNFIO-SFARGHIU³, DELPHINE FARLAY², NINA ATTIK^4,5, SYLVAIN MEILLE¹, JéRôME CHEVALIER¹, DAVID MITTON⁶

BIOCELL, Vol.46, No.10, pp. 2209-2216, 2022, DOI:10.32604/biocell.2022.022290 - 13 June 2022

Abstract To develop durable bone healing strategies through improved control of bone repair, it is of critical importance to understand the mechanisms of bone mechanical integrity when in contact with biomaterials and implants. Bone mechanical integrity is defined here as the adaptation of structural properties of remodeled bone in regard to an applied mechanical loading. Accordingly, the authors present why future investigations in bone repair and regeneration should emphasize on the matrix surrounding the osteocytes. Osteocytes are mechanosensitive cells considered as the orchestrators of bone remodeling, which is the biological process involved in bone homeostasis. These… More >

WENJUAN XU¹, GUANGMING DAI¹, YIFEI LYU², YINING ZHANG¹, XIAOLIN TU^1,*

BIOCELL, Vol.46, No.4, pp. 1097-1104, 2022, DOI:10.32604/biocell.2022.017505 - 15 December 2021

Abstract This study established a method for isolating large numbers of high-purity osteocytes from high-density bone. Bone fragments derived from mice tibia and femurs were alternately digested with type I collagenase and EDTA nine times, and the digested cells and bone chips (BC) were cultured, digested, and passaged when cells were fully grown. The types of cells obtained were identified by morphology, viable cell counts, alkaline phosphatase staining, and biochemical activity analyses, and specific osteocyte and osteoblast markers were evaluated by quantitative real-time polymerase chain reaction. Our results showed that among the cells obtained from the… More >

J. B. Litzenberger^{1, 2}, P. Tummala² , C. R. Jacobs^{1, 2}

Molecular & Cellular Biomechanics, Vol.3, No.4, pp. 219-220, 2006, DOI:10.32604/mcb.2006.003.219

Abstract This article has no abstract. More >