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    ARTICLE

    Differential Responses of Cultured MC3T3-E1 Cells to Dynamic and Static Stimulated Effect of Microgravity in Cell Morphology, Cytoskeleton Structure and Ca2+ Signaling

    Mingzhi Luo1,2, Peili Yu1, Yang Jin3, Zhili Qian1, Yue Wang1, Jingjing Li1, Peng Shang2*, Linhong Deng1*

    Molecular & Cellular Biomechanics, Vol.13, No.2, pp. 137-157, 2016, DOI:10.3970/mcb.2016.013.155

    Abstract Random positioning machine (RPM) and diamagnetic levitation are two essential ground-based methods used to stimulate the effect of microgravity in space life science research. However, the force fields generated by these two methods are fundamentally different, as RPM generates a dynamic force field acting on the surface in contact with supporting substrate, whereas diamagnetic levitation generates a static force field acting on the whole body volume of the object (e.g. cell). Surprisingly, it is hardly studied whether these two fundamentally different force fields would cause different responses in mammalian cells. Thus we exposed cultured MC3T3-E1… More >

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