LEI SHI1,#, JIANSHI YIN2,#, YU CHEN1, JIANGANG SHI1, JINHAO MIAO1,*
BIOCELL, Vol.48, No.9, pp. 1389-1400, 2024, DOI:10.32604/biocell.2024.049562
Abstract Background: Ossification of the posterior longitudinal ligament (OPLL) is a prevalent condition in orthopedics. While death-associated protein kinase 2 (DAPK2) is known to play roles in cellular apoptosis and autophagy, its specific contributions to the advancement of OPLL are not well understood. Methods: Ligament fibroblasts were harvested from patients diagnosed with OPLL. Techniques such as real-time reverse transcriptase-polymerase chain reaction (RT-qPCR) and Western blot analysis were employed to assess DAPK2 levels in both ligament tissues and cultured fibroblasts. The extent of osteogenic differentiation in these cells was evaluated using an alizarin red S (ARS) staining. Additionally,… More >
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