LEI SHI^1,#, JIANSHI YIN^2,#, YU CHEN¹, JIANGANG SHI¹, JINHAO MIAO^1,*

BIOCELL, Vol.48, No.9, pp. 1389-1400, 2024, DOI:10.32604/biocell.2024.049562 - 04 September 2024

Abstract Background: Ossification of the posterior longitudinal ligament (OPLL) is a prevalent condition in orthopedics. While death-associated protein kinase 2 (DAPK2) is known to play roles in cellular apoptosis and autophagy, its specific contributions to the advancement of OPLL are not well understood. Methods: Ligament fibroblasts were harvested from patients diagnosed with OPLL. Techniques such as real-time reverse transcriptase-polymerase chain reaction (RT-qPCR) and Western blot analysis were employed to assess DAPK2 levels in both ligament tissues and cultured fibroblasts. The extent of osteogenic differentiation in these cells was evaluated using an alizarin red S (ARS) staining. Additionally,… More >

FABIANA ROCHA ARAÚJO¹, BRUNO MACHADO BERTASSOLI², ISABELLA CRISTINA SOUZA FÉLIX¹, DOUGLAS MARINHO ABREU¹, NATáLIA MELO OCARINO¹, AMANDA MARIA SENA REIS³, JUNEO FREITAS SILVA⁴, ROGéRIA SERAKIDES^1,*

BIOCELL, Vol.47, No.2, pp. 423-430, 2023, DOI:10.32604/biocell.2023.023689 - 18 November 2022

Abstract To evaluate the characteristics and synthesis activity of osteoblasts extracted from the calvaria of offspring of rats exposed to maternal hyperthyroidism. Twelve adult Wistar rats were divided into two groups, one control and one treated with daily administration of L-thyroxine by an orogastric tube (50 µg/animal/day) during pregnancy. Three days after delivery and confirmation of the mothers’ hyperthyroidism, the offspring were euthanized for the extraction of osteoblasts from the calvaria. At 7, 14, and 21 days, proliferation activity was assessed using MTT assay, while alkaline phosphatase (ALP) activity was assessed by the BCIP/NBT method. At… More >