YUSHI YANG^1,#, CHUJIAO HU^2,#, SHAN LEI³, XIN BAO³, ZHIRUI ZENG^3,*, WENPENG CAO^4,*

Oncology Research, Vol.32, No.12, pp. 1921-1934, 2024, DOI:10.32604/or.2024.046191 - 13 November 2024

Abstract Background: The heterogeneity of prognosis and treatment benefits among patients with gliomas is due to tumor microenvironment characteristics. However, biomarkers that reflect microenvironmental characteristics and predict the prognosis of gliomas are limited. Therefore, we aimed to develop a model that can effectively predict prognosis, differentiate microenvironment signatures, and optimize drug selection for patients with glioma. Materials and Methods: The CIBERSORT algorithm, bulk sequencing analysis, and single-cell RNA (scRNA) analysis were employed to identify significant cross-talk genes between M2 macrophages and cancer cells in glioma tissues. A predictive model was constructed based on cross-talk gene expression, and… More >

TIANYUN HAN^#, ZHONG LI^#, LUONING ZHANG, LINSHEN XIE^*

BIOCELL, Vol.48, No.11, pp. 1625-1638, 2024, DOI:10.32604/biocell.2024.055029 - 07 November 2024

Abstract Objective: The study aimed to explore the protective mechanism of Ganoderic acid A (GAA) in renal fibrosis and to verify that GAA can ameliorate renal fibrosis by regulating the Niemann-pick C1-like 1 (NPC1L1) gene. Methods: Transforming growth factor beta1 (TGF-β1) was used to treat Human Kidney-2 (HK-2) cells to establish a renal fibrosis model. The differentially expressed genes in the control (CTRL) group, TGF-β1 group, and TGF-β1 + GAA group were screened via transcriptome sequencing technology and verified by qPCR and Western blot experiments. The NPC1L1 gene overexpression plasmid was constructed. The expression levels of N-cad, E-cad,… More >

Xiaodong Shi^*, Tingyu Wang, Sui Ai, Jiasi Li

Phyton-International Journal of Experimental Botany, Vol.93, No.10, pp. 2571-2581, 2024, DOI:10.32604/phyton.2024.056606 - 30 October 2024

Abstract Cotton rose (Hibiscus mutabilis) is a well-known ornamental plant that produces large flowers of vibrant colors. However, metabolites in H. mutabilis flowers with vibrant color have not been fully understood. By performing a combined analysis of metabolomics and transcriptomics data, we here explored mechanisms for the production of primary active compounds in this plant. Multivariate statistics unveiled differences in flavonoid metabolism between white and pink flowers, with pink flowers exhibiting a greater flavonoid abundance. The white-to-pink transition of cotton rose flowers may be attributed to pelargonidin-3-O-glucoside formation. On examining the expression of genes related to the structure More > Graphic Abstract

Yinyu Xu¹, Zheng Li¹, Qian Wen², Hao He¹, Pengtu Shi¹, Wenhua Zhou^2,*

Phyton-International Journal of Experimental Botany, Vol.93, No.9, pp. 2367-2382, 2024, DOI:10.32604/phyton.2024.047949 - 30 September 2024

Abstract The diversity of metabolites produced in fermented pine needles at different stages of fermentation has rarely been investigated. In the present study, untargeted metabolomic analysis using GC-MS and LC-MS was performed to detect metabolites in fermented pine needles at different fermentation stages. A total of 30 samples of pine needles fermented first with yeast (Saccharomyces cerevisiae) and then with a mixed bacterial culture of Lactobacillus fermentum CECT5716 and Bifidobacterium Breve M16V, were used to detect differential metabolites at different stages of fermentation. Pearson’s correlation analysis was used to determine correlations between metabolites and key microbial communities. A… More >

KERAN JIA¹, YANHAO ZHANG¹, MENGYU JIANG², MENGGE CUI², JIA WANG², JIAJIA ZHANG², HUIHAI ZHAO², MENGYAN LI², HUA WANG², QUANMING ZOU^1,#,*, HAO ZENG^1,#,*

BIOCELL, Vol.48, No.9, pp. 1355-1368, 2024, DOI:10.32604/biocell.2024.051383 - 04 September 2024

Abstract Objective: Candida albicans is a common fungal pathogen that triggers complex host defense mechanisms, including coordinated innate and adaptive immune responses, to neutralize invading fungi effectively. Exploring the immune microenvironment has the potential to inform the development of therapeutic strategies for fungal infections. Methods: The study analyzed individual immune cell profiles in peripheral blood mononuclear cells from Candida albicans-infected mice and healthy control mice using single-cell transcriptomics, fluorescence quantitative PCR, and Western blotting. We investigated intergroup differences in the dynamics of immune cell subpopulation infiltration, pathway enrichment, and differentiation during Candida albicans infection. Results: Our findings indicate that infiltration… More >

Fuxin Li¹, Enyan Chen¹, Xinxin Chen¹, Jingyu Jia¹, Hemin Wang¹, Jie Zhang¹, Jianrui Sun¹, Xin Li^1,2,3,*

Phyton-International Journal of Experimental Botany, Vol.93, No.8, pp. 2095-2113, 2024, DOI:10.32604/phyton.2024.054231 - 30 August 2024

Abstract Salt stress inhibits plant growth and affects the biosynthesis of its secondary metabolites. Flavonoids are natural compounds that possess many important biological activities, playing a significant role in the medicinal activity of Eucommia ulmoides (E. ulmoides). To investigate the mechanism by which salt stress affects the biosynthesis of flavonoids in E. ulmoides, a comprehensive analysis of metabolomics and transcriptomics was conducted. The results indicated that salt stress led to the wilting and darkening of E. ulmoides leaves, accompanied by a decrease in chlorophyll levels, and significantly induced malondialdehyde (MDA) and relative electrical conductivity. During salt stress, most metabolites in… More >

Rittick Maity^1,2, Muhammad Khairul Imran bin Ahmad Shuhaimi³, Kumarasamy Sudhakar^3,4,5,*, Amir Abdul Razak³

Energy Engineering, Vol.121, No.9, pp. 2331-2361, 2024, DOI:10.32604/ee.2024.051576 - 19 August 2024

Abstract The United Nations’ Sustainable Development Goals (SDGs) highlight the importance of affordable and clean energy sources. Solar energy is a perfect example, being both renewable and abundant. Its popularity shows no signs of slowing down, with solar photovoltaic (PV) panels being the primary technology for converting sunlight into electricity. Advancements are continuously being made to ensure cost-effectiveness, high-performing cells, extended lifespans, and minimal maintenance requirements. This study focuses on identifying suitable locations for implementing solar PV systems at the University Malaysia Pahang Al Sultan Abdullah (UMPSA), Pekan campus including buildings, water bodies, and forest areas.… More > Graphic Abstract

Pengcheng Li^1,2,#, Tianze Zhu^1,#, Yunyun Wang^1,2, Shuangyi Yin¹, Xinjie Zhu¹, Minggang Ji¹, Wenye Rui¹, Houmiao Wang¹, Zefeng Yang^1,2,*, Chenwu Xu^1,2,*

Phyton-International Journal of Experimental Botany, Vol.93, No.7, pp. 1441-1453, 2024, DOI:10.32604/phyton.2024.052219 - 30 July 2024

Abstract The nutritional composition and overall quality of maize kernels are largely determined by the key chemical components: protein, oil, and starch. Nevertheless, the genetic basis underlying these nutritional quality traits during grain filling remains poorly understood. In this study, the concentrations of protein, oil, and starch were studied in 204 recombinant inbred lines resulting from a cross between DH1M and T877 at four different stages post-pollination. All the traits exhibited considerable phenotypic variation. During the grain-filling stage, the levels of protein and starch content generally increased, whereas oil content decreased, with significant changes observed between… More >

Dongbo Wu^1,#, Shuang Qu^2,#, Lianwen Shen², Shengqun Chen², Xia Jiang^2,3, Ai Rao¹, Yuxue Zhao^2,*, Yueyun Wang^1,*

Phyton-International Journal of Experimental Botany, Vol.93, No.7, pp. 1455-1466, 2024, DOI:10.32604/phyton.2024.051652 - 30 July 2024

Abstract Cymbidium goeringii is an economically important ornamental plant, and flower color is one of the main features of C. goeringii that contributes to its high economic value. To clarify the molecular mechanisms underlying the role of anthocyanins in mediating differences in color among varieties, liquid chromatography–tandem mass spectrometry was used to perform anthocyanin-targeted metabolomics of seven C. goeringii varieties, including ‘Jin Qian Yuan’ (JQY), ‘Jin Xiu Qian Yuan’ (JXQY), ‘Miao Jiang Su Die’ (MJSD), ‘Qian Ming Su’ (QMS), ‘Shi Chan’ (SC), and ‘Yang Ming Su’ (YMS), as well as the C. goeringii. We detected 64 anthocyanins, including cyanidins, delphinidins,… More >

Bairu Li¹, Jingyu Jia¹, Hemin Wang¹, Jiaju Sun¹, Enyan Chen¹, Xin Li^1,2,3,*

Phyton-International Journal of Experimental Botany, Vol.93, No.5, pp. 885-900, 2024, DOI:10.32604/phyton.2024.050450 - 28 May 2024

Abstract Trypsin, a novel superoxide scavenger, significantly enhances the storage quality of Hylocereus undatus (H. undatus). To elucidate the preservation mechanism of trypsin on H. undatus, a widely targeted metabolomic analysis, and transcriptomics analysis were conducted. Firstly, a total of 453 metabolites were identified, with organic acids and their derivatives constituting the largest proportion (25%). Amino acids and their metabolites, prominent among organic acids, were further analyzed. Among them, 73 metabolites were associated with amino acids, and 37 exhibited significant differences. The most enriched Kyoto Encyclopedia of Genes and Genomes (KEGG) pathway was arginine biosynthesis (map00220), with polyamine metabolites… More >