YU TANG¹, WENXIAO HU^2,*, YAJUN PENG¹, XIANGDONG LING²

BIOCELL, Vol.48, No.10, pp. 1503-1516, 2024, DOI:10.32604/biocell.2024.052692 - 02 October 2024

Abstract Background: Podocyte injury is crucial in diabetic kidney disease (DKD) progression, and the mechanism remains unclear. The previous studies indicated Diosgenin played a key role in inhibiting podocyte injury progression. However, more research is needed to explore Diosgenin in inhibiting-molecular mechanisms in the process of podocyte injury. Methods: The content of Diosgenin in HeShenwan was detected by High-Performance Liquid Chromatography-mass spectrometry (HPLC-MS) method. The podocyte injury model was constructed by high glucose (HG)-induced mpc5 cells. The Cell Counting Kit-8 (CCK-8) assay was utilized to evaluate the activity of mpc5 cells. Pyroptosis in mpc5 cells was… More > Graphic Abstract

JIAN LI¹, QINGQING WANG², XIAOYING ZHANG^1,*

BIOCELL, Vol.47, No.6, pp. 1307-1316, 2023, DOI:10.32604/biocell.2023.028714 - 19 May 2023

Abstract Scutellarin (SCU) is a herbal flavonoid glucuronide with multiple pharmacological activities, including anti-oxidant, anti-inflammation, vascular relaxation, anti-platelet, and myocardial protection. However, the effect of SCU on complete Freund’s adjuvant (CFA)-induced rheumatoid arthritis (RA) had not been studied. In this study, we investigated the beneficial effects of SCU in the CFA-induced RA mice model and the anti-arthritic activity was evaluated by paw edema. Enzyme-linked immunosorbent assay (ELISA) was carried out to evaluate the plasma levels of immunoglobulin (Ig)G, IgE, tumor necrosis factor (TNF)-α, interleukin (IL)-1β, IL-6, receptor activator of nuclear factor-κB ligand (RANKL), and osteoprotegerin (OPG).… More >

SITONG QIAN^1,2,#, YING FANG^1,#, CHENGYUN YAO^1,#, YONGSHENG WANG^3,#, ZHI ZHANG¹, XIAOHUA WANG¹, JIN GAO¹, YONG FENG¹, LEI SUN¹, RUNYUE ZOU², GUOREN ZHOU^1,*, JINJUN YE^1,*, RUIXUE XIA^4,*, HONGPING XIA^5,6,*

Oncology Research, Vol.30, No.2, pp. 53-64, 2022, DOI:10.32604/or.2022.026302 - 05 January 2023

Abstract Previous studies have shown that PRDX5 and Nrf2 are antioxidant proteins related to abnormal reactive oxidative species (ROS). PRDX5 and Nrf2 play a critical role in the progression of inflammations and tumors. The combination of PRDX5 and Nrf2 was examined by Co-immunoprecipitation, western blotting and Immunohistochemistry. H₂O₂ was applied to affect the production of ROS and induced multi-resistant protein 1 (MRP1) expression in NSCLC cells. The zebrafish models mainly investigated the synergistic effects of PRDX5 and Nrf2 on lung cancer drug resistance under oxidative stress. We showed that PRDX5 and Nrf2 form a complex and significantly More >

Jinfeng Qu^*, Lei Zhang^†, Lanyu Li^*, Yujie Su^*

Oncology Research, Vol.27, No.1, pp. 81-88, 2019, DOI:10.3727/096504018X15202988139874

Abstract This study investigated the tumor-suppressive role of miR-148b in regulating endoplasmic reticulum metalloprotease 1 (ERMP1) expression and the oxidative stress response in endometrial cancer cells. Human endometrial cancer RL95-2 cells were used and transfected with miR-148b mimic, miR-148b inhibitor, or their scrambled negative control. Thereafter, the transfection efficiency was determined by RT-qPCR, and cell proliferation was assessed by MTT assay. The dual-luciferase reporter assay, Western blot, and RT-qPCR were conducted to determine the target gene of miR-148b. ERMP1 is a putative target of miR-148b, and thereby the overexpression and downregulation of ERMP1 on the proliferation… More >