LIXU JIANG1, LIN NING2, CHUNCHAO PU1, ZIXIN WANG1, BIFANG HE1,3, JIAN HUANG1,*
BIOCELL, Vol.46, No.2, pp. 547-557, 2022, DOI:10.32604/biocell.2021.016500
- 20 October 2021
Abstract As nucleic acid-guided endonucleases, some prokaryotic Argonautes have been used as programmable
nucleases. Natronobacterium gregoryi Argonaute (NgAgo) has also been proposed for gene editing, but this remains
very controversial. Until now, the endogenous nucleic acids that bind to NgAgo in Natronobacterium gregoryi sp2
(N. gregoryi sp2) have not been characterized. We expressed the conserved PIWI domain of NgAgo and used it to
induce anti-PIWI antibody. We also cultured the N. gregoryi sp2 strain and performed immunoprecipitation,
chromatin immunoprecipitation (ChIP), and RNA immunoprecipitation (RIP) assays. The nucleic acids that
endogenously bound NgAgo in N. gregoryi sp2 cells were sequenced and analyzed.… More >
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