JING WANG1,#, JUNFENG XU2,#, YINGRAN YANG1, YOUZHENG QIU1, SHANSHAN ZHANG3, NING WANG1,3,*
BIOCELL, Vol.48, No.9, pp. 1343-1353, 2024, DOI:10.32604/biocell.2024.051673
Abstract Objective: This study aimed to elucidate the influence of IFN-gamma (IFN-γ) in neuroblastoma (NB) cells and reveal its potential underlying molecular mechanism. Methods: The Cell Counting Kit-8, Transwell apparatus, and flow cytometry were employed to assess cellular viability, migratory capacity, invasive potential, and apoptotic rates, respectively. RNA-seq combined with bioinformatics analysis revealed differentially expressed genes (DEGs) and their possible biological functions. Protein levels were determined by western blot analysis. Results: IFN-γ treatment resulted in diminished cell viability, mitigated migratory and invasive capabilities, and augmented apoptotic activity in the SK-N-BE (2) cell line, whereas it exhibited the… More >
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