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  • Open Access

    ARTICLE

    Cloning and Functional Validation of Mung Bean VrPR Gene

    Xiaokui Huang1, Yingbin Xue1, Aaqil Khan1, Hanqiao Hu1, Naijie Feng1,2,*, Dianfeng Zheng1,2,*

    Phyton-International Journal of Experimental Botany, Vol.92, No.8, pp. 2369-2382, 2023, DOI:10.32604/phyton.2023.027457 - 25 June 2023

    Abstract For the purpose of functional validation, the mung bean (Vigna radiata) VrPR gene was cloned and overexpressed in Arabidopsis thaliana. The findings revealed that the ORF of VrPR contained 1200 bp, in which 399 amino acids were encoded. Bioinformatics analysis showed that the VrPR protein belonged to the NADB Rossmann superfamily, which was one of the non-transmembrane hydrophilic proteins. VrPR was assumed to have 44 amino acid phosphorylation sites and be contained in chloroplasts. The VrPR secondary structure comprised of random coil, α helix, β angle, and extended chain, all of which were quite compatible with the anticipated tertiary structure. More >

  • Open Access

    ARTICLE

    Integrated Transcriptome and Small RNA Sequencing Analyses Reveals Insights into the Molecular Mechanism of Seed Germination in Mung Bean

    Yanyan Pu1,#, Liwen Wang2,#, Leilei Li3, Yujun Si4, Shubin Xie2, Yunzhe Cong1, Dong Wang1, Yongchao Gong1, Rumei Tian1, Xue Chen3, Xiaoyan Zhang1, Min Liu1, Hanfeng Ding1,3,*, Nana Li1,3,*

    Phyton-International Journal of Experimental Botany, Vol.92, No.6, pp. 1793-1812, 2023, DOI:10.32604/phyton.2023.026822 - 11 April 2023

    Abstract During the life cycle of a plant, seed germination is crucial. Upon ingestion of water, the dry seeds resumed energy metabolism and cellular repair. To dissect the complex mechanisms at the very beginning of seed germination, two approaches including transcriptome and small RNA sequencing were conducted during the water imbibition process of mung bean seeds compared with dry seed. The transcriptome sequencing analysis identified 10,108 differentially expressed genes (DEGs) between dry and imbibed mung bean seeds. KEGG enrichment analyses demonstrated numerous DEGs involved in hormone signaling pathways, carbohydrate, and energy metabolism. Out of the total… More >

  • Open Access

    ARTICLE

    Drought Tolerance in Mung Bean is Associated with the Genotypic Divergence, Regulation of Proline, Photosynthetic Pigment and Water Relation

    Mohammad Rafiqul Islam1, Mohd Mostofa Kamal2, Mohammad Faruk Hossain3, Jamil Hossain4, Mohammad Golam Azam4,11, Mst Masuma Akhter5, M. Kamrul Hasan6, Ibrahim Al-Ashkar7, Khalid F. Almutairi7, Ayman EL Sabagh8,*, Md Atikur Rahman9, Muhammad Aamir Iqbal10, Mohammad Sohidul Islam6,*

    Phyton-International Journal of Experimental Botany, Vol.92, No.3, pp. 955-981, 2023, DOI:10.32604/phyton.2023.025138 - 29 November 2022

    Abstract Drought is one of the critical conditions for the growth and productivity of many crops including mung bean (Vigna radiata L. Wilczek). Screening of genotypes for variations is one of the suitable strategies for evaluating crop adaptability and global food security. In this context, the study investigated the physiological and biochemical responses of four drought tolerant (BARI Mung-8, BMX-08010-2, BMX-010015, BMX-08009-7), and four drought sensitive (BARI Mung-1, BARI Mung-3, BU Mung-4, BMX-05001) mung bean genotypes under wellwatered (WW) and water deficit (WD) conditions. The WW treatment maintained sufficient soil moisture (22% ± 0.5%, i.e., 30% deficit… More >

  • Open Access

    ARTICLE

    Basal Application of Zinc to Improve Mung Bean Yield and Zinc-Grains Biofortfication

    Aroosa Kanwal1, Muhammad Bismillah Khan1, Mubshar Hussain1,2,*, Muhammad Naeem1, Muhammad Shahid Rizwan3, Muhammad Zafar-ul-Hye4

    Phyton-International Journal of Experimental Botany, Vol.89, No.1, pp. 87-96, 2020, DOI:10.32604/phyton.2020.07845 - 01 March 2020

    Abstract Worldwide, the dietary deficiency of zinc (Zn) is prevailing in almost all arid and semi-arid regions. Zinc deficiency is not only the major constraint of lower yield, but also dietary Zn deficiency in cereals grains may cause increasing malnutrition and chronic health problems in human. Exogenous application of Zn through basal soil nutrition might be a useful option to recover Zn deficiency in mung bean. Therefore, field study was conducted to optimize the optimum level and method of Zn nutrition to enhance crop yield and Zn biofortification of mung bean through basal application. Zinc was… More >

  • Open Access

    ARTICLE

    Germinating seeds of the mung bean, Vigna radiata (Fabaceae), as a model for the preliminary evaluation of cytotoxic effects of drugs

    VIJAY L. KUMAR*, ABHISHEK SINGHAL

    BIOCELL, Vol.33, No.1, pp. 19-24, 2009, DOI:10.32604/biocell.2009.33.019

    Abstract Cytotoxic properties of plant extracts and drugs being developed for cancer treatment are usually evaluated by a variety of in vivo and in vitro tests carried out in animal or plant based models. In the present study we have evaluated the possibility of using the germinating mung beans (Vigna radiata), for rapid and inexpensive screening of drugs exhibiting cytotoxic properties. Mung beans were allowed to germinate either in tap water or in different drug solutions, and parameters like percent germination, increase in radicle length, change in seedling weight and mitotic index of apical root meristems were determined… More >

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