Mutamba Ropafadzo Peace¹, Thobeka Madide^1,2, Ntethelelo Sibiya^1,*

BIOCELL, Vol.49, No.11, pp. 2069-2091, 2025, DOI:10.32604/biocell.2025.068017 - 24 November 2025

Abstract The pathogenesis of insulin resistance is influenced by environmental factors, genetic predispositions, and several medications. Various drugs used to manage multiple ailments have been shown to induce insulin resistance, which could lead to Type II Diabetes mellitus (T2DM). Central to drug-induced insulin resistance is mitochondrial dysfunction. Amongst disturbed pathways in drug-induced mitochondrial toxicity is mitophagy, a process that removes dysfunctional mitochondria through the lysosomal pathways to maintain mitochondrial quality. A balance must always be maintained between mitochondrial dynamics and mitophagy, as any alterations may contribute to the pathogenesis of metabolic diseases such as diabetes mellitus.… More >

Lingjuan Linghu^1,#, Hongying Zhou^2,#, Gang Zheng¹, Tao Yi^1,*

Oncology Research, Vol.33, No.9, pp. 2421-2434, 2025, DOI:10.32604/or.2025.063724 - 28 August 2025

Abstract Objectives: Ovarian cancer, a leading cause of gynecological malignancy-related mortality, is characterized by limited therapeutic options and a poor prognosis. Although pyrimethamine has emerged as a promising candidate demonstrating efficacy in treating various tumors, the precise mechanisms of its antitumor effects remain obscure. This study was specifically designed to investigate the mode of action underlying the antitumor effects of pyrimethamine in preclinical settings. Methods: The effects of pyrimethamine on cellular proliferation were meticulously assessed using both the cell counting kit 8 (CCK-8) assay and the colony formation assay, with the effects further confirmed in a… More >

Kristina A. Kritskaya, Evgeniya I. Fedotova, Alexander D. Nadeev^*, Alexey V. Berezhnov^*

BIOCELL, Vol.49, No.3, pp. 451-464, 2025, DOI:10.32604/biocell.2025.061624 - 31 March 2025

Abstract Objective: Activation of mitophagy is a promising option to overcome the mitochondrial malfunction that accompanies many diseases. Herein, we investigate the mechanisms underlying the ability of sodium lactate and pyruvate to initiate mitophagy, from the perspective of action on mitochondrial network and expression levels. Methods: Fluorescent and confocal microscopy was used to assess key cell parameters characterizing the state of the mitochondrial network and the level of mitophagy in human fibroblasts carrying mutations in genes encoding LRRK2 and PINK1 after the combined application of lactate and pyruvate and after direct acidification. qRT-PCR was used to… More >

XITONG ZHAO, TIANJUAN JU, XINWEI LI, CHANGFENG LIU, LULU WANG^*, LI-AN WU^*

BIOCELL, Vol.48, No.1, pp. 47-64, 2024, DOI:10.32604/biocell.2023.045591 - 30 January 2024

Abstract Dental stem cells (DSCs) have attracted significant interest as autologous stem cells since they are easily accessible and give a minimal immune response. These properties and their ability to both maintain self-renewal and undergo multi-lineage differentiation establish them as key players in regenerative medicine. While many regulatory factors determine the differentiation trajectory of DSCs, prior research has predominantly been based on genetic, epigenetic, and molecular aspects. Recent evidence suggests that DSC differentiation can also be influenced by autophagy, a highly conserved cellular process responsible for maintaining cellular and tissue homeostasis under various stress conditions. This… More >

YANPING JIN¹, JIANPING QIU¹, XIUFANG LU¹, YAN MA¹, GUOWEI LI^2,*

Oncology Research, Vol.31, No.2, pp. 169-179, 2023, DOI:10.32604/or.2023.027815 - 10 April 2023

Abstract Previous study revealed that ferritin heavy chain-1 (FTH1) could regulate ferritinophagy and affect intracellular Fe²⁺ content in various tumors, while its N6-methyladenosine (m6A) RNA methylation was closely related the prognosis of ovarian cancer patients. However, little is known about the role of FTH1 m6A methylation in ovarian cancer (OC) and its possible action mechanisms. In this study we constructed FTH1 m6A methylation regulatory pathway (LncRNA CACNA1G-AS1/IGF2BP1) according to related bioinformatics analysis and research, through clinical sample detections we found that these pathway regulatory factors were significantly up-regulated in ovarian cancer tissues, and their expression levels were More >