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    ARTICLE

    Construction and characterization of a metagenomic DNA library from the rhizosphere of wheat (Triticum aestivum)

    Hernández-León R1, M Martínez-Trujillo2, E Valencia-Cantero1, G Santoyo1

    Phyton-International Journal of Experimental Botany, Vol.81, pp. 133-137, 2012, DOI:10.32604/phyton.2012.81.133

    Abstract Rhizospheric soil of wheat plants contains a high diversity of microorganisms, and therefore, comprises a large reservoir for discovering genes with diverse agro-biotechnological applications. In this work, we constructed an E. coli metagenomic library based on bacterial artificial chromosome (BAC) clones with large genomic inserts from metagenomic DNA from the rhizosphere of wheat plants. The average of the DNA cloned segments varies from 5 to 80 kb, with an average size of 38 kb. Random clones were end-sequenced and homology results showed that the clonation of metagenomic DNA codes mainly for metabolic and catalytic functions (40%), More >

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