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  • Open Access

    REVIEW

    Revolutionizing Metabolic Engineering in Cannabis sativa L.: Harnessing the Power of Hairy Root Culture

    Md. Injamum-Ul-Hoque1,2, Md. Mahfuzur Rahman2, Nayan Chandra Howlader3, Soosan Tavakoli4, Md. Mezanur Rahman5, Shahin Imran6, Mallesham Bulle7, S. M. Ahsan2,4,*, Hyong Woo Choi4,8,*

    Phyton-International Journal of Experimental Botany, Vol.94, No.12, pp. 3805-3826, 2025, DOI:10.32604/phyton.2025.069827 - 29 December 2025

    Abstract Cannabis sativa is highly valued for its use in fiber production, medicine, and recreational products. Its secondary metabolites (SM) are renowned for their wide range of health benefits and psychoactive properties. While much of the existing research has focused on cannabinoid production in the plant’s aerial parts, particularly the leaves and flowers, the root system remains understudied in terms of its SM profile. One promising in vitro approach for metabolite production involves the use of ‘hairy roots (HRs)’. These roots mimic the phytochemical profile of native roots but grow more efficiently and yield higher quantities of metabolites.… More >

  • Open Access

    REVIEW

    From Model Organism to Pharmaceutical Powerhouse: Innovative Applications of Yeast in Modern Drug Research

    Xiaobing Li1,2, Yongsheng Liu1, Limin Wei1, Li Rao1, Jingxin Mao1,*, Xuemei Li3,*

    BIOCELL, Vol.49, No.5, pp. 813-832, 2025, DOI:10.32604/biocell.2025.062124 - 27 May 2025

    Abstract Yeast-based models have become a powerful platform in pharmaceutical research, offering significant potential for producing complex drugs, vaccines, and therapeutic agents. While many current drugs were discovered before fully understanding their molecular mechanisms, yeast systems now provide valuable insights for drug discovery and personalized medicine. Recent advancements in genetic engineering, metabolic engineering, and synthetic biology have improved the efficiency and scalability of yeast-based production systems, enabling more sustainable and cost-effective manufacturing processes. This paper reviews the latest developments in yeast-based technologies, focusing on their use as model organisms to study disease mechanisms, identify drug targets,… More >

  • Open Access

    REVIEW

    Metabolic engineering and genome editing strategies for enhanced lipid production in microalgae

    ANJANI DEVI CHINTAGUNTA1, SAMUDRALA PRASHANT JEEVAN KUMAR2, NUNE SATYA SAMPATH KUMAR1,*

    BIOCELL, Vol.48, No.8, pp. 1181-1195, 2024, DOI:10.32604/biocell.2024.050540 - 02 August 2024

    Abstract Depleting global petroleum reserves and skyrocketing prices coupled with succinct supply have been a grave concern, which needs alternative sources to conventional fuels. Oleaginous microalgae have been explored for enhanced lipid production, leading towards biodiesel production. These microalgae have short life cycles, require less labor, and space, and are easy to scale up. Triacylglycerol, the primary source of lipids needed to produce biodiesel, is accumulated by most microalgae. The article focuses on different types of oleaginous microalgae, which can be used as a feedstock to produce biodiesel. Lipid biosynthesis in microalgae occurs through fatty acid More >

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