Carlos Erik Medina Santos1, Carmen Nathaly López Hurtado1, Bruno Rivas Santiago1, Roberto Gonzalez-Amaro3, Yolanda Guadalupe Cataño Cañizales1, Margarita de la Luz Martínez Fierro2, José Antonio Enciso-Moreno1, Mariana Haydee García Hernández1
European Cytokine Network, Vol.27, No.3, pp. 68-74, 2016, DOI:10.1684/ecn.2016.0379
Abstract The aim of this study was to evaluate the effects of the LL-37, HNP-1 and HBD2/3 peptides on
cytokine and MMP production in human polymorphonuclear cells, mononuclear cells and chondrocytes. The levels
of cytokines in supernatants from mononuclear and polymorphonuclear cell cultures were measured with a cytometric
bead array by flow cytometry. Likewise, the levels of metalloproteinase/MMP-1, 3, and 13 were measured in
supernatants from chondrocyte cultures using an ELISA. The expression of RANKL on lymphocytes was analyzed
by flow cytometry. We observed increased levels of TNF-α, IL-6 and IL-10 in mononuclear and polymorphonuclear
cell… More >
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