Xin Qi^1,#, Yu Wang^1,#, Xing Zhang¹, Xiaoshuang Wei¹, Xinyang Liu¹, Zhennan Wang¹, Zhenhui Wang^1,*, Fenglou Ling^2,*

Phyton-International Journal of Experimental Botany, Vol.93, No.7, pp. 1767-1779, 2024, DOI:10.32604/phyton.2024.052844 - 30 July 2024

Abstract DNA methylation is an important epigenetic regulatory mechanism, it regulates gene expression by recruiting proteins involved in gene repression or by inhibiting the binding of transcription factor(s) to DNA. In this study, a novel methyltransferase 2a gene (Zmet2a) was cloned in maize and identified by polymerase chain reaction-base (PCR-base) using a bioinformatics strategy. The Zmet2a cDNA sequence is 2739 bp long and translates to 912 amino acid peptides. The Zmet2a protein revealed that it contains BAH and CHROMO structural domains, is a non-transmembrane protein that is hydrophilically unstable, and has no signal peptide structure. Meanwhile, we verified More >

Xiaojie Jing^1,2,3,#, Chunyan Li^1,2,3,#, Chengjuan Luo^1,2,3, Chaonan Yao^1,2,3, Jiahao Zhang^1,2,3, Tingting Zhu^1,2,3, Jiuguang Wang^1,2,3, Chaoxian Liu^1,2,3,*

Phyton-International Journal of Experimental Botany, Vol.92, No.3, pp. 707-723, 2023, DOI:10.32604/phyton.2023.024338 - 29 November 2022

Abstract The zinc finger homeodomain (ZF-HD) genes belong to the homeobox gene family, playing critical roles in flower development and stress response. Despite their importance, however, to date there has been no genome-wide identification and characterization of the ZF-HD genes that are probably involved in stress responses in maize. In this study, 24 ZF-HD genes were identified, and their chromosomal locations, protein properties, duplication patterns, structures, conserved motifs and expression patterns were investigated. The results revealed that the ZF-HD genes are unevenly distributed on nine chromosomes and that most of these genes lack introns. Six and… More >

M. Ashraf Ahangar¹, Shabir Hussain Wani^1,*, Zahoor A. Dar², Jan Roohi¹, Fayaz Mohiddin¹, Monika Bansal³, Mukesh Choudhary⁴, Sumit K. Aggarwal⁴, S. A. Waza¹, Khursheed Ahmad Dar⁵, Ayman El Sabagh^6,7, Celaleddin Barutcular⁸, Omer Konuşkan⁹, Mohammad Anwar Hossain^10,*

Phyton-International Journal of Experimental Botany, Vol.91, No.10, pp. 2111-2133, 2022, DOI:10.32604/phyton.2022.020721 - 30 May 2022

Abstract Maize is cultivated extensively throughout the world and has the highest production among cereals. However, Northern corn leaf blight (NCLB) disease caused by Exherohilum turcicum, is the most devastating limiting factor of maize production. The disease causes immense losses to corn yield if it develops prior or during the tasseling and silking stages of crop development. It has a worldwide distribution and its development is favoured by cool to moderate temperatures with high relative humidity. The prevalence of the disease has increased in recent years and new races of the pathogen have been reported worldwide. The… More >

Lingyan Hu, Haiyan Chen, Guangqun Zhang, Zihao Yu, Kai Yan, Fangdong Zhan, Yongmei He^*

Phyton-International Journal of Experimental Botany, Vol.91, No.9, pp. 1933-1944, 2022, DOI:10.32604/phyton.2022.020041 - 13 May 2022

Abstract Indoleacetic acid (IAA) is an important regulator that plays a crucial role in plant growth and responses to abiotic stresses. In the present study, a sand cultivation experiment was carried out to investigate the effects of IAA at different concentrations (0, 0.01, 0.1, 0.5, 1, and 2.5 mmol/L) on maize growth, root morphology, mineral elements (Ca, Mg) and Cd uptake under 20 mg/kg Cd stress. The results showed that 0.01 mmol/L is the optimal IAA concentration for enhancing the Cd tolerance of maize. Compared with the control treatment, 0.01 mmol/L IAA promoted maize growth, with… More >

Jing Qu¹, Shuang Liu², Peng Jiao², Zhenzhong Jiang², Jianbo Fei², Shuyan Guan^1,*, Yiyong Ma^1,*

Phyton-International Journal of Experimental Botany, Vol.91, No.8, pp. 1709-1719, 2022, DOI:10.32604/phyton.2022.018854 - 14 April 2022

Abstract To develop cold-tolerant maize germplasms and identify the activation of INDUCER OF CRT/DRE-BINDING FACTOR EXPRESSION (ICE1) expression in response to cold stress, RT-PCR was used to amplify the complete open reading frame sequence of the ICE1 gene and construct the plant expression vector pCAMBIA3301-ICE1-Bar. Immature maize embryos and calli were transformed with the recombinant vector using Agrobacterium tumefaciens-mediated transformations. From the regenerated plantlets, three T1 lines were screened and identified by PCR. A Southern blot analysis showed that a single copy of the ICE1 gene was integrated into the maize (Zea mays L.) genomes of the three T1 generations.… More >

Hameed Gul¹, Mengya Qian¹, Mohammad G. Arabzai^1,2, Tianhui Huang¹, Qiannan Ma¹, Fangyu Xing¹, Wan Cao¹, Tingting Liu¹, Hong Duan¹, Qianlin Xiao^1,*, Zhizhai Liu^1,*

Phyton-International Journal of Experimental Botany, Vol.91, No.7, pp. 1429-1443, 2022, DOI:10.32604/phyton.2022.019842 - 14 March 2022

Abstract Kernel size-related traits, including kernel length, kernel width, and kernel thickness, are critical components in determining yield and kernel quality in maize (Zea mays L.). Dissecting the phenotypic characteristics of these traits, and discovering the candidate chromosomal regions for these traits, are of potential importance for maize yield and quality improvement. In this study, a total of 139 F2:3 family lines derived from EHel and B73, a distinct line with extremely low ear height (EHel), was used for phenotyping and QTL mapping of three kernel size-related traits, including 10-kernel length (KL), 10-kernel width (KWid), and 10-kernel… More >

Castañón-Nájera G¹, L Latorunerie-Moreno², YA Gálvez Muñoz³

Phyton-International Journal of Experimental Botany, Vol.86, pp. 290-295, 2017, DOI:10.32604/phyton.2017.86.290

Abstract Among the different types of abiotic stresses, water is the most damaging for crops. In the current research, the effect of water stress, induced with Polyethylene Glycol 8000 (PEG8000) and Sodium chloride (NaCl) solutions, was studied in maize genotypes. A randomized block design (RBD) with a factorial arrangement and four replications of 25 seeds each was used. The studied maize genotypes were RC₂C39, RC₂C51 (wild varieties of the State of Tabasco backcrossed with the endogamic line CML247 of the International Center for the Improvement of Maize and Wheat), wild Tamulte, VS536, and Asgrow 7573 hybrid, study… More >

Iqbal A¹, MA Iqbal¹, A Iqbal¹, Z Aslam¹, M Maqsood¹, Z Ahmad², N Akbar¹, HZ Khan¹, RN Abbas¹, RD Khan¹, G Abbas¹, M Faisal¹

Phyton-International Journal of Experimental Botany, Vol.86, pp. 84-88, 2017, DOI:10.32604/phyton.2017.86.084

Abstract Seed inoculation with bacterial species has the potential to increase yield and agro-qualitative attributes of forage crops. This study determined the response of forage maize to three plant growth promoting rhizobacteria [PGPR1 (Azotobacter chroococcum), PGPR2 (Pseudomonas flourescens) and PGPR3 (Bacillus megaterium)] inoculated individually and in different combinations (PGPR1+2, PGPR1+3, PGPR2+3 and PGPR1+2+3). A non-inoculated treatment was kept as a control. We used a completely randomized block design with four replicates. The PGPR1+2+3 treatment showed an outstanding performance by improving yield attributes, green forage yield, dry matter biomass, crude protein and total ash. The same treatment gave the lowest More >

Yescas CP¹, MA Segura C¹, L Martínez C², VP Álvarez R¹, JA Montemayor T¹, JA Orozco V¹, JE Frías R¹

Phyton-International Journal of Experimental Botany, Vol.84, No.2, pp. 272-279, 2015, DOI:10.32604/phyton.2015.84.272

Abstract The scarcity of water in arid and semiarid regions of the world is a problem that every day increases by climate change. The subsurface drip irrigation (SDI) and changes in population density of plants are alternatives that can be used to make a sustainable use of water. Therefore, the objectives of this study were to determine the combination that allows for an increased corn performance and efficient use of water without losing the quality of forage. Three different irrigation levels were applied through a system of a SDI at three different densities of forage maize… More >

Kandus¹ M, D Almorza³, R Boggio Ronceros², JC Salerno¹

Phyton-International Journal of Experimental Botany, Vol.79, pp. 39-46, 2010, DOI:10.32604/phyton.2010.79.039

Abstract Our objective was to determine the genotype-environment interaction (GxE) in a hybrid integrated by maize lines either carrying or not balanced lethal systems. Experiments were conducted in three locations over a period of two years considering each yearlocation combination as a different environment. Yield data were analysed using the Additive Main Effects and Multiplicative Interaction (AMMI) model and the Sites Regression Analysis (SREG). Results were represented by biplots. The AMMI analysis was the best model for determining the interaction. More >