JING JIN1,#, XINGHUA WANG2,#, XIAOXIAO ZHENG3, JIAHUA LAN3, LI ZHENG3, YING CAI3, HUI CHEN4, HONGWEI WANG5,*, LIFANG ZHENG6,*
BIOCELL, Vol.47, No.3, pp. 619-626, 2023, DOI:10.32604/biocell.2023.026016
Abstract Ischemic cerebrovascular disease is a leading cause of death globally and is often exacerbated by cerebral ischemic/reperfusion injury (CIRI). The exact mechanisms underlying I/R injury are unclear. In this study, we aimed to determine the role of m6A-modified methylase complex methyltransferase-like 3 (METTL3) in cerebral ischemia-reperfusion (I/R) injury. We found that m6A and METTL3 levels increased in OGD/RX-induced mouse astrocytes-cerebellar (MA-C) and the brain of middle cerebral artery occlusion (MCAO) model mice. METTL3 siRNA treatment reduced OGD-RX-induced MAC cell viability and proliferation, which increased with METTL3 over-expression. Flow cytometry analysis showed that silencing METTL3 significantly enhanced OGD/RX-induced MAC apoptosis, which… More >
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