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    ARTICLE

    Influence of the microenvironment on gene and protein expression of odontogenic-like and osteogenic-like cells

    ANDREA P. RODRIGUEZ, HIDETSUGU TSUJIGIWA, MEHMET GUNDUZ, BEYHAN CENGIZ, NORIYUKI NAGAI, RYO TAMAMURA, SILVIA S. BORKOSKY, TOHRU TAKAGI, MIHO INOUE, HITOSHI NAGATSUKA*

    BIOCELL, Vol.33, No.1, pp. 39-47, 2009, DOI:10.32604/biocell.2009.33.039

    Abstract Progenitor cells play an important biological role in tooth and bone formation, and previous analyses during bone and dentine induction have indicated that they may be a good alternative for tissue engineering. Thus, to clarify the influence of the microenvironment on protein and gene expression, MDPC23 cells (mouse dental papilla cell line) and KUSA/A1 cells (bone marrow stromal cell line) were used, both in vitro cell culture and in intra-abdominal diffusion chambers implanted in 4-week-old male immunodefficient mice (SCID mice). Our results indicate that KUSA/A1 cells differentiated into osteoblast-like cells and induced bone tissue inside the More >

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