Hongliang Yang*1,
Lei Yan†1,
Kai Sun‡, Xiaodong Sun†, Xudong Zhang,§ Kerui Cai†, Tiejun Song*
Oncology Research, Vol.27, No.3, pp. 359-369, 2019, DOI:10.3727/096504018X15220594629967
Abstract This study aimed to explore the effects of lncRNA BCAR4 on the viability and aggressiveness of non-small cell
lung cancer (NSCLC) cells. qRT-PCR was used to determine the expression of BCAR4 and GLI2 downstream
genes in NSCLC tissues and cell lines. Chromatin isolation by RNA purification (CHIRP) and Western blot
were employed to measure the expression of the GLI2 downstream proteins. Ki-67 expression in nude mice
tumors was tested by immunohistochemistry. MTT assay, wound healing assay, and Transwell assay were used
to assess NSCLC cell viability and aggressiveness, respectively. Tumor xenograft was conducted to determine
the… More >
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