ELMIRA YAKUPOVA¹, VALENTINA BABENKO^1,2, ALEXEY BOCHARNIKOV¹, KSENIYA FEDULOVA¹, DENIS SILACHEV^1,2, EGOR PLOTNIKOV^1,2,*

BIOCELL, Vol.48, No.12, pp. 1827-1834, 2024, DOI:10.32604/biocell.2024.058496 - 30 December 2024

Abstract Background: The active use of stem and progenitor cells in the therapy of various diseases requires the development of approaches for targeted modification of their properties. One such approach is the induction of a pro- or anti-inflammatory phenotype. Methods: In this study, we investigated the effect of a pro-inflammatory environment in vitro on multipotent mesenchymal stromal cells (MSC) by incubation with lipopolysaccharide (LPS). iCELLigence real-time cell analysis system was used for monitoring cell culture growth. Cell energy metabolism was assessed using the Seahorse XFp Analyzer. For the rat stroke experiment, we used a photoinduced thrombosis (PT)… More >

JINGRONG SONG^#, JUAN KANG^#, WEI LV, YAN DONG, XIAOYING ZHANG^*

BIOCELL, Vol.45, No.3, pp. 723-731, 2021, DOI:10.32604/biocell.2021.014453 - 03 March 2021

Abstract Bronchial asthma is a common chronic inflammatory disease characterized by airway hyperresponsiveness (AHR), inflammatory cell infiltration, and airway remodeling. F-box/WD repeat-containing protein 7 (FBXW7), an E3 ubiquitin ligase, is required for various endothelial functions, such as cell migration, inflammation, and endothelial integrity. This study aimed to investigate the role of FBXW7 in lipopolysaccharide (LPS)-induced epithelial barrier impairment in bronchial epithelial cells in vitro. By using lentivirus-based technology, FBXW7 was overexpressed or silenced (24 h) in human bronchial epithelial (16HBE) cells, which were treated with LPS or not (24 h). Immunoprecipitation (IP) detection and Western blot… More >

EUN NA¹, MYOUNGWON CHOI², INDAL PARK², SUNYOUNG LIM^3,*

BIOCELL, Vol.44, No.2, pp. 193-199, 2020, DOI:10.32604/biocell.2020.08648 - 27 May 2020

Abstract We investigated the effect of black sea bream extracts on changes in pro-inflammatory and anti-inflammatory cytokines induced by lipopolysaccharide (LPS) in murine splenocytes. The levels of pro-inflammatory cytokines [interleukin (IL)-6, tumor necrosis factor-α (TNF-α), IL-12/IL-23(p40) and IL-17A] and anti-inflammatory cytokines [IL-4, IL-10 and interferon-γ (IFN-γ)] were assessed. Incubation of murine splenocytes with acetone+methylene chloride (A+M) and methanol (MeOH) extracts significantly decreased LPS-induced IL-6, IL-12/IL-23(p40) and IL-17A productions after 6 h incubation (p < 0.05). The A+M and MeOH extracts significantly increased LPS-induced IL-4 and IFN-γ productions at 48 and 72 h incubation (p < 0.05). More >