WENJUAN ZHU¹, ZHENGZHENG JU², FAN CUI^2,*

BIOCELL, Vol.48, No.9, pp. 1379-1388, 2024, DOI:10.32604/biocell.2024.052205 - 04 September 2024

Abstract Objective: Diabetic nephropathy (DN) is a deleterious microangiopathy of diabetes, constituting a critical determinant of fatality in diabetic patients. This work is purposed to disclose the effects and modulatory mechanism of BTG anti-proliferation factor 2 (BTG2) during the pathological process of DN. Methods: BTG2 expression in kidney tissues of diabetic mice and high glucose (HG)-exposed human proximal tubular cell line HK-2 was assessed with Western blot and RT-qPCR. The diabetic mice model was constructed by streptozotocin injection and confirmed by the blood glucose level beyond 16.7 mmol/L. Hematoxylin and eosin (H&E) staining and measurement of… More >

ANJANI DEVI CHINTAGUNTA¹, SAMUDRALA PRASHANT JEEVAN KUMAR², NUNE SATYA SAMPATH KUMAR^1,*

BIOCELL, Vol.48, No.8, pp. 1181-1195, 2024, DOI:10.32604/biocell.2024.050540 - 02 August 2024

Abstract Depleting global petroleum reserves and skyrocketing prices coupled with succinct supply have been a grave concern, which needs alternative sources to conventional fuels. Oleaginous microalgae have been explored for enhanced lipid production, leading towards biodiesel production. These microalgae have short life cycles, require less labor, and space, and are easy to scale up. Triacylglycerol, the primary source of lipids needed to produce biodiesel, is accumulated by most microalgae. The article focuses on different types of oleaginous microalgae, which can be used as a feedstock to produce biodiesel. Lipid biosynthesis in microalgae occurs through fatty acid More >

HONGBIAO WU^*, DONGFANG LIU

BIOCELL, Vol.48, No.6, pp. 971-980, 2024, DOI:10.32604/biocell.2024.049466 - 10 June 2024

Abstract Background: Colorectal cancer (CRC) represents a substantial risk to public health. Bevacizumab, the first US FDA-approved antiangiogenic drug (AAD) for human CRC treatment, faces resistance in patients. The role of lipid metabolism, particularly through OPA3-regulated lipid droplet production, in overcoming this resistance is under investigation. Methods: The protein expression pattern of OPA3 in CRC primary/normal tissues was evaluated by bioinformatics analysis. OPA3-overexpressed SW-480 and HCT-116 cell lines were established, and bevacizumab resistance and OPA3 effects on cell malignancy were examined. OPA3 protein/mRNA expression and lipid droplet-related genes were measured with Western blot and qRT-PCR. OPA3… More > Graphic Abstract

JIAN TANG¹, CHENQIANG ZHU¹, YUN CHEN¹, YUNLONG WU¹, MING HE¹, YI ZHOU², MINGHUA XIE^2,*

BIOCELL, Vol.48, No.4, pp. 639-651, 2024, DOI:10.32604/biocell.2024.047562 - 09 April 2024

Abstract Background: The specific impact of sphingolipid metabolism on developing hepatocellular Carcinoma (HCC) remains unclear. This study aims to explore the relationship between sphingolipid metabolism and HCC prognosis, immune response, and drug sensitivity. Methods: Data were obtained from The Cancer Genome Atlas (TCGA)-Hepatocellular Carcinoma (LIHC) and Gene Expression Omnibus (GEO, GSE14520 datasets). 47 sphingolipid metabolism genes were obtained from the Kyoto Encyclopedia of Genes and Genomes (KEGG) database. After classifying HCC samples using the Non-negative Matrix Factorization (NMF) clustering method, differentially expressed genes were screened. Then, 8 risk genes were obtained by univariate analysis, survival random forest… More > Graphic Abstract

MINGHONG WEI^1,#, CHENG XIAO^1,2,#, JIAN WU^1,3, LIHONG QIN^1,3, HONGLIANG LIU^1,3, YANG CAO^1,*, YUMIN ZHAO^1,3,*

BIOCELL, Vol.47, No.9, pp. 2115-2123, 2023, DOI:10.32604/biocell.2023.030364 - 28 September 2023

Abstract Background: The regulatory genes related to lipid metabolism affect the intramuscular fat (IMF) content and improve meat quality traits. Mediator Complex Subunit 4 (MED4), a vitamin D receptor protein, affects vitamin D, livestock growth, carcass traits, and triglyceride deposition. However, the physiological function of the MED4 gene on bovine adipocyte differentiation remains unknown. Methods: This study explored the function of the MED4 gene in preadipocyte differentiation in Chinese Red Steppe cattle. The overexpression plasmid and the interference sequences of the MED4 gene were constructed to detect the effects of the MED4 gene on adipogenesis and biomakers using More >

Huwida A. A. Abdel-Kader¹, Naeima Yousef¹, Mohammad Anwar Hossain^2,*, Mona F. A. Dawood^1,*

Phyton-International Journal of Experimental Botany, Vol.92, No.7, pp. 1955-1974, 2023, DOI:10.32604/phyton.2023.028583 - 29 May 2023

Abstract The extensive use of nanoparticles (NPs) in diverse applications causes their localization to aquatic habitats, affecting the metabolic products of primary producers in aquatic ecosystems, such as algae. Synthesized calcium oxide nanoparticles (CaO NPs) are of the scarcely studied NPs. Thus, the current work proposed that the exposure to CaO NPs may instigate metabolic pathway to be higher than that of normally growing algae, and positively stimulate algal biomass. In this respect, this research was undertaken to study the exposure effect of CaO NPs (0, 20, 40, 60, 80, and 100 µg mL⁻¹ ) on the… More >

PAULA MARIELA GONZÁLEZ^1,2, JOAQUIN CABRERA^1,2, SUSANA PUNTARULO^1,2,*

BIOCELL, Vol.47, No.5, pp. 1177-1189, 2023, DOI:10.32604/biocell.2023.027107 - 10 April 2023

Abstract

The purpose of this review is to briefly summarize the central role of iron (Fe) in terms of cellular alterations of the oxidative/protective balance with special emphasis on its possible involvement in ferroptosis-dependent disruption in aquatic organisms. In ferroptotic cells or tissues, the intracellular Fe level increases; meanwhile the treatment with Fe chelators limits ferroptosis. Eukaryotic algae can assimilate Fe from the environment through several mechanisms, and aquatic animals incorporate dissolved Fe and Fe bound to both inorganic particles and organic matter. The central role of lipid peroxidation mediating ferroptosis was demonstrated in some algae

… More >

Xue Huang^1,*, Zeyu Chen¹, Ruizhao Yang¹, Guangzhu Feng²

Journal of Renewable Materials, Vol.11, No.5, pp. 2301-2319, 2023, DOI:10.32604/jrm.2023.025888 - 13 February 2023

Abstract 1,3-Dioleoyl-2-palmitoylglycerol (OPO) has been a hotspot of functional oils research in recent years, but due to the high cost of sn-1,3 specific lipase in enzymatic synthesis and the lack of biocatalyst stability, large-scale industrial application is difficult. In this study, the prepared magnetic ZnFe₂O₄ was functionalized with dopamine to obtain ZnFe₂O₄@PDA, and the nano-biocatalyst ZnFe₂O₄@PDA@RML was prepared by immobilizing sn-1,3 specific lipase of Rhizomucor miehei lipase (RML) via a cross-linking method. The existence of RML on ZnFe₂O₄@PDA was confirmed by XRD, FTIR, SEM, and TEM. This strategy proved to be simple and effective because the lipase immobilized on… More > Graphic Abstract

Bejoy Abraham¹, Jesna Mohan², Linu Shine³, Sivakumar Ramachandran^3,*

CMES-Computer Modeling in Engineering & Sciences, Vol.136, No.1, pp. 87-106, 2023, DOI:10.32604/cmes.2023.023384 - 05 January 2023

Abstract Tear film, the outermost layer of the eye, is a complex and dynamic structure responsible for tear production. The tear film lipid layer is a vital component of the tear film that provides a smooth optical surface for the cornea and wetting the ocular surface. Dry eye syndrome (DES) is a symptomatic disease caused by reduced tear production, poor tear quality, or excessive evaporation. Its diagnosis is a difficult task due to its multifactorial etiology. Out of several clinical tests available, the evaluation of the interference patterns of the tear film lipid layer forms a More >

Qian Liu¹, Sayikal Duyxanale¹, Yongqian Tang², Xinyu Shen¹, Yuanlai Zhao¹, Xinru Ma¹, Shuai Shao¹, Chenfei Liang^1,*

Phyton-International Journal of Experimental Botany, Vol.92, No.3, pp. 943-954, 2023, DOI:10.32604/phyton.2022.023225 - 29 November 2022

Abstract Chinese hickory (Carya cathayensis Sarg.) is an important economic forest in Southeastern China. A large amount of hickory husk waste is generated every year but with a low proportion of returning. Meanwhile, intensive management has resulted in soil degradation of Chinese hickory plantations. This study aims to investigate the effects of three Chinese hickory husk returning modes on soil amendment, including soil acidity, soil nutrition, and microbial community. The field experiment carried out four treatments: control (CK), hickory husk mulching (HM), hickory husk biochar (BC), and hickory husk organic fertilizer (OF). The phospholipid fatty acid (PLFA)… More >