MIAOMIAO LI^1,#, ZILIN ZHENG^1,#, JUNYI KE¹, JIEYI LUO¹, FAN JIANG¹, YANXIA QU¹, BING ZHU², YINGHUA LI^2,*, LIANDONG ZUO^1,*

BIOCELL, Vol.47, No.6, pp. 1397-1405, 2023, DOI:10.32604/biocell.2023.027971

Abstract Background: Nano-selenium has been widely used in antiviral and anticancer therapy, and has the advantages of good targeting and low toxicity. For the first time, we combined male reproduction with nano-selenium to investigate its antioxidant effect. This study investigated the protective effect of lentinan functionalized selenium nanoparticles on oxidative stress injury of the hydrogen peroxide (H₂O₂)-induced Leydig cell line, TM3. Methods: The suitable concentration of nano-selenium treatment to promote cell proliferation was also discussed. The concentration of 4 μM could significantly promote the growth of TM3 cells. Oxidative stress damage was caused using an 800 μM concentration of hydrogen peroxide.… More >

MIAOMIAO LI^1,#, DANYANG CHEN^2,#, JUNYI KE¹, RUILIN ZHENG², JINGYAO SU², ZILIN ZHENG¹, JIEYI LUO¹, HANRAN MAI¹, FAN JIANG¹, YANXIA QU¹, XIAOQIONG GU¹, BING ZHU², YINGHUA LI^2,*, LIANDONG ZUO^1,*

BIOCELL, Vol.47, No.2, pp. 401-408, 2023, DOI:10.32604/biocell.2023.025154

Abstract A H₂O₂-induced oxidative stress injury cell model was established to investigate the antioxidant effect of nano-selenium on mouse spermatocyte lines and the regulation mechanism of the expression level and activity of selenium-containing antioxidant enzymes induced by oxidative stress. A safe and effective nano-drug system of functionalized selenium-containing nanoparticles (SeNPs) was developed with lentinan (LNT) (SeNPs@LNT). Mice spermatocyte line GC2-spg cells were treated with SeNPs@LNT (1, 2, 4, 8, 16, 32 μM) for 24–72 h to evaluate the cytotoxicity of selenium. GC2-spg cells were randomly divided into the following groups: control, hydrogen peroxide (H₂O₂), SeNPs@LNT, and H₂O₂+SeNPs@LNT groups. H₂O₂+SeNPs@LNT group was… More >