Hernández-Camacho S1, E Pérez-Molphe-Balch1, AG Alpuche-Solís2, JF Morales-Domínguez1
Phyton-International Journal of Experimental Botany, Vol.86, pp. 151-162, 2017, DOI:10.32604/phyton.2017.86.151
Abstract Dehydrins or Group 2 Late Embryogenesis Abundant (LEA) proteins play an important role in the response and adaptation to different types of abiotic stresses such as droughts, high salinity and low temperatures. Using PCR techniques, we identified three gene fragments that encoded dehydrin-like proteins in three cactispecies Opuntia ficus-indica (OpfiDHN-like), Leuchtenbergia principis (LepDHN-like) and Mammillaria bombycina (MabDHN-like). Bioinformatic sequence analysis showed an identity between 96 and 97% with the Opuntia streptacantha dehydrin 1 (OpsDHN1) gene, demonstrating that the amplified fragments corresponded to dehydrin-like gene sequences, and that the designed oligonucleotides were effective for similar gene amplification in different cacti… More >
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