Yinyu Xu¹, Zheng Li¹, Qian Wen², Hao He¹, Pengtu Shi¹, Wenhua Zhou^2,*

Phyton-International Journal of Experimental Botany, Vol.93, No.9, pp. 2367-2382, 2024, DOI:10.32604/phyton.2024.047949 - 30 September 2024

Abstract The diversity of metabolites produced in fermented pine needles at different stages of fermentation has rarely been investigated. In the present study, untargeted metabolomic analysis using GC-MS and LC-MS was performed to detect metabolites in fermented pine needles at different fermentation stages. A total of 30 samples of pine needles fermented first with yeast (Saccharomyces cerevisiae) and then with a mixed bacterial culture of Lactobacillus fermentum CECT5716 and Bifidobacterium Breve M16V, were used to detect differential metabolites at different stages of fermentation. Pearson’s correlation analysis was used to determine correlations between metabolites and key microbial communities. A… More >

Touseef Hussain^1,*, Abrar A. Khan¹, Heba I. Mohamed^2,*

Phyton-International Journal of Experimental Botany, Vol.92, No.3, pp. 783-799, 2023, DOI:10.32604/phyton.2022.026045 - 29 November 2022

Abstract Fusarium dry rot is considered to be the most critical soilborne and postharvest disease that damages potato tubers worldwide when they are stored for a long time. This study was performed to demonstrate the effect of crude extract, culture filtrate, and cell suspension obtained from the bacterium Bacillus subtilis HussainT-AMU, on the net house and field. From oil-contaminated soil, through the serial dilution method, biosurfactant bacteria were isolated on nutrient agar medium. To isolate and screen the prospective biosurfactant strain, various biosurfactant screening methods were used. Standard protocols were carried out for morphological, molecular, and chemical… More >

Kesheng Lin^1,3,#, Zhengqi Wang^2,#, Li Wang^1,3, Jiawen Zhou^1,3, Lijuan Han^1,3, Jingjing Wen², Tingxia Dong², Ran Duan^2,*, Ning Li^1,3,*

Phyton-International Journal of Experimental Botany, Vol.92, No.2, pp. 423-437, 2023, DOI:10.32604/phyton.2022.023763 - 12 October 2022

Abstract Prohibited pesticide residues have become one of the main factors affecting the quality and safety of Lycii Fructus, However, rarely studies focus on the rapid determination of these residues. Here, a total of 30 kinds of prohibited pesticide residues were determined by ultra-performance liquid chromatography tandem triple quadrupole mass spectrometry (UPLC-MS/MS) in five different process ways. Pretreatment methods, chromatographic separation and detection conditions in mass spectrometry were all optimized accordingly. Among the five different pretreatment methods, the first and third solid phase extraction failed to provide high recoveries of sulfosulfuron compounds (both lower than 60%).… More >

Lixian Wu^1,#, Yongquan Li^1,#, Qiaoguang Li², Jingxuan Li¹, Qinghua Lao¹, Yucheng Zhong¹, Hui Zhang^1,*, Xiu Hu^1,*

Journal of Renewable Materials, Vol.11, No.2, pp. 975-989, 2023, DOI:10.32604/jrm.2022.023547 - 22 September 2022

Abstract This study aimed to explore the anti-bacterial and anti-fungal activities of extracts from different parts of plants in the Zingiberaceae family. The inhibitory rate, minimum inhibitory concentration (MIC), and minimum bactericidal concentration (MBC) of leaf and stem, and root and rhizome extracts from Alpinia katsumadai Hayata, Alpinia oxyphylla Miq × Alpinia henryi K. Schumann, Alpinia oblongifolia Hayata, Alpinia nigra (Gaertn.) Burtt, Amomum villosum Lour, Alpinia zerumbet (Pers.) Burtt. et Smith and Alpinia oxyphylla Miq were determined using the fungus cake method and double dilution method. The seven Zingiberaceae plants exhibited characteristic antibacterial activities against pathogenic bacteria and fungi. At a 1.5 mg mL⁻¹, A. zerumbet… More >

Yu Zai^*, Shaohua Liu

Phyton-International Journal of Experimental Botany, Vol.92, No.1, pp. 225-236, 2023, DOI:10.32604/phyton.2022.022576 - 06 September 2022

Abstract Kosteletzkya virginica (K. virginica) is used for revegetation of salt-affected coastal tidal flats and as a raw material of biodiesel. K. virginica root tuber, a biowaste with low economic value, is rich in bioactive compounds. This study aimed to extract and identify flavonoids from K. virginica root tubers. The optimal extraction conditions were 1/25 (w/v) solid/liquid ratio, 40% ethanol concentration at 40°C for 60 min. Under these conditions, 65.2 ± 3.7 mg/g total flavonoid content was extracted from the roots, which were collected from salinized soil in late autumn of the third year. Antioxidant activity was evaluated through 1,1-diphenyl-2-picrylhydrazyl, More >

J. David García-Ortíz¹, Rebeca González-Centeno¹, María Alejandra Torres-Tapia², J. A. Ascacio-Valdés¹, José Espinoza-Velázquez², Raúl Rodríguez-Herrera^1,*

Phyton-International Journal of Experimental Botany, Vol.91, No.5, pp. 929-941, 2022, DOI:10.32604/phyton.2022.018368 - 24 January 2022

Abstract Because of the growing worldwide demand for maize grain, new alternatives have been sought for breeding of this cereal, e.g., development of polyembryonic varieties, which agronomic performance could positively impact the grain yield per unit area, and nutritional quality. The objectives of this study were to (1) determine the phytochemicals present in the embryo and endosperm of grain from maize families with high, low, and null polyembryony frequency, which were planted at different locations, and (2) state the relationship between these compounds and seed germination. The extracted phytochemicals from corn were identified by HPLC-MS. The… More >

Feng Liu^1,2,3, Zilan Ma^1,2,3, Marcos A. Caraballo-Ortiz⁴, Hui Zhang⁵, Xu Su^1,2,3, Yuping Liu^1,2,3,*

Phyton-International Journal of Experimental Botany, Vol.89, No.4, pp. 1083-1090, 2020, DOI:10.32604/phyton.2020.011239 - 09 November 2020

Abstract Pedicularis longiflora var. tubiformis (Orobanchaceae) is an abundant parasitic herb mainly found in the Xiaopohu wetland of the Qinghai Lake Basin in Northwestern China. The species has an important local medicinal value, and in this study, we evaluated the chemical profile of its stems, leaves and seeds using mass spectrometry. Dried samples of stems, leaves and seeds were grinded, weighted, and used for a series of extractions with an ultrasonic device at room temperature. The chemical profiles for each tissue were determined using Gas Chromatography-Mass Spectrometry (GC-MS) and Liquid ChromatographyMass Spectrometry (LC-MS). Twenty-seven amino acids and organic… More >