ZULONG SHENG^*, YANRU HE, JUNYAN CAI, YUQIN JI, YUYU YAO, GENSHAN MA

BIOCELL, Vol.48, No.9, pp. 1369-1377, 2024, DOI:10.32604/biocell.2024.049905

Abstract Background: Myocardial infarction (MI) is known worldwide for its important disabling features, including myocarditis and cardiomyocyte apoptosis. It is believed that microRNA (miRNA) has a role in the cellular processes of apoptosis and myocarditis, and miR-219a-5p has been found to suppress the inflammatory response. However, unknown is the precise mechanism by which miR-219a-5p contributes to MI. Methods: We measured the expression of miR-219a-5p and evaluated its effects on target proteins, inflammatory factors, and apoptosis in a mouse model of MI. Echocardiography was utilized to examine the MI clinical index, and triphenyl tetrazolium chloride staining was More >

RAN PAN^1,#, JIAYAN MAO^2,#, YUELIANG ZHENG³, WEI CHEN², JUNPING GUO^1,*, LIJUN WANG^3,*

BIOCELL, Vol.48, No.3, pp. 431-441, 2024, DOI:10.32604/biocell.2023.046484

Abstract Background: Sepsis, a type of systemic disease, can impact nearly all organs, tissues and cells. Among them, endothelial cells are amongst the first to be affected and respond to the insult. In this study, we investigated the protective effects of microRNA-30a-5p (miR-30a-5p) on human pulmonary microvascular endothelial cells (HPMECs) treated with lipolysaccharide (LPS). Methods: An in vitro model of sepsis was established in HPMECs with the use of LPS. Transfecting with different tools (mimetic and inhibitor) to modify miR-30a-5p expression. Cell viability, proliferation and apoptosis were detected by the CCK-8 assay, the EdU kit and fluorescence… More >

ABDELAZIZ ADAM IDRISS ARBAB^1,3,#, CHUNQING YIN^4,#, XUBIN LU¹, YAN LIANG¹, ISMAIL MOHAMED ABDALLA¹, AMER ADAM IDRIS³, TIANLE XU^1,2, YONGJIANG MAO¹, ZHANGPING YANG^1,2,*

BIOCELL, Vol.46, No.11, pp. 2443-2454, 2022, DOI:10.32604/biocell.2022.020865

Abstract Mastitis is a common inflammatory cow mammary infection; that causes significant economic loss in dairy industry. Given the interesting connection between metformin’s anti-inflammatory function and mastitis model induced by LTA in pbMECs, our objective was to prove that metformin was beneficial in suppressing proinflammatory response induced by LTA through modulation of mitogen-activated protein kinase (MAPK) and nuclear factor kappa B (NF-κB) signaling pathways and activation of peroxisome proliferator-activated receptor-γ (PPARγ) in pbMECs. The proliferation of cells and mRNA expression were measured using EdU assay and quantitative reverse transcriptase-polymerase chain reaction (qRT-PCR). Immunoblotting and immunofluorescence analysis… More >

LINMEI ZHENG¹, RONG TANG², ^1,*, ZHONGYI ZHOU³

BIOCELL, Vol.45, No.5, pp. 1231-1240, 2021, DOI:10.32604/biocell.2021.015752

Abstract Pre-eclampsia is characterized by an excessive maternal inflammatory response. The cholinergic anti-inflammatory pathway (CAP) has been shown as the efferent arm of a vagal reflex with the potential to limit inflammatory responses. Therefore, in this study, the CAP regulation through the nervous vagal stimulation (VNS) reduced the severity of NG-nitro-L-arginine methyl ester (L-NAME)-induced pre-eclampsia was determined in a rat model. Rats were given 125 mg/kg/day of L-NAME via subcutaneous injection on gestational day (GD) 10–16. In addition, the rats were treated by active or sham electrical stimulation once a day during GD 13–19. Systolic blood… More >

Hong Liu^1,#,*, Luyao Ma^1,#, Jinghang Li^1,#, Bingqi Sun², Siqiang Zheng³, Yongfeng Shao^1,*

Congenital Heart Disease, Vol.16, No.5, pp. 443-455, 2021, DOI:10.32604/CHD.2021.015588

Abstract Background: This study evaluated differential inflammatory response to cardiopulmonary bypass reoxygenation in tetralogy of Fallot repair. Methods: We performed a retrospective study at a cardiovascular center from 2012 to 2018, including 500 patients aged 1 week–18 years who received complete repair of tetralogy of Fallot. Patients were grouped according to tertiles of preoperative RVOT gradient on echocardiography into mild, moderate, and severe stenosis. We measured the highest perfusate oxygenation (PpO2) during aortic occlusion as independent variable. Primary outcome was systemic inflammatory response syndrome (SIRS) within 7 days postoperatively or the time of death or discharge. Results: Overall, rate… More >

Siqiang Tian^1,#, Yao Jiang^2,#, Ying Wang¹, Jie Song^1,*

Oncologie, Vol.22, No.1, pp. 13-21, 2020, DOI:10.32604/oncologie.2020.012492

Abstract To explore the effects of dexmedetomidine combined with remifentanil anesthesia and propofol combined with remifentanil anesthesia on perioperative inflammatory response and pulmonary function in patients with lung cancer. Totally 128 patients admitted to our hospital for lung cancer surgery were taken as the research object of this experiment. Among them, 67 cases were sedated with dexmedetomidine combined with remifentanil (Group A), and 61 cases were sedated with propofol combined with remifentanil (Group B). The changes of vital signs, respiratory interval (RR), blood oxygen saturation (SpO2) and blood sugar before and after sedation were recorded. The… More >