Jo Van Damme^1,$, Ghislain Opdenakker^2,$, Sam Van Damme³, Soffe Struyf¹

European Cytokine Network, Vol.34, No.1, pp. 1-9, 2023, DOI:10.1684/ecn.2023.0484

Abstract Polyclonal antisera from patients have been at the basis of the description of autoimmune diseases and today monoclonal antibodies are widely used in the therapy of cancer and many inflammatory diseases. How antisera and antibodies in combination with traditional in vitro and in vivo biological test systems have been instrumental reagents for the discovery of new cytokines is illustrated here for interleukin-1, -6 and -8. Furthermore, widely used immunological detection/quantification systems, such as ELISAs and multiplex assays, based on the use of either polyclonal or monoclonal antibodies, are often fraught with misinterpretations, because the results are affected More >

Ting Liu¹, Fang Liu¹, Lei-Wen Peng, Li Chang, Yong-Mei Jiang

Oncology Research, Vol.26, No.5, pp. 817-826, 2018, DOI:10.3727/096504017X15130753659625

Abstract Peritoneal macrophages (PMs) are the major cell type of peritoneal cells that participate in multiple aspects of innate and acquired immunity in the peritoneal cavity. PMs have an ability to release a large amount of proinflammatory and anti-inflammatory cytokines and therefore play a critical role in regulating the differentiation of innate immune cells and inflammatory T cells. Accumulating studies demonstrate that the immunological reactions and inflammatory responses of PMs are strongly related to the pathogenic processes of various inflammatory diseases and abdominal cancers. Consequently, the regulation of PM activation has gradually emerged as a promising More >

Paola Migliorini¹, Isabella Del Corso¹, Cristina Tommasi¹, Diana Boraschi²

European Cytokine Network, Vol.20, No.3, pp. 108-111, 2009, DOI:10.1684/ecn.2009.0164

Abstract Schnitzler syndrome is a rare disease characterised by chronic urticaria and arthralgia. The recent evidence that the IL-1 receptor antagonist IL-1Ra could induce rapid and complete remission of Schnit-zler symptoms has pointed to IL-1 as a major pathological factor in this disease. To examine the possibility that Schnitzler syndrome may be considered to be an autoinflammatory disease, in this study we measured the serum levels of IL-18, another cytokine of the IL-1 family that is cleaved by caspase-1, in two recently diag-nosed Schnitzler patients before and after treatment with IL-1Ra. In parallel, mRNA expression of More >