CINZIA SIGNORINI^*, GIULIA COLLODEL, ELENA MORETTI

BIOCELL, Vol.46, No.4, pp. 881-884, 2022, DOI:10.32604/biocell.2022.018062

Abstract The manuscript deals with our studies and experiences in the assessment of cytoskeleton in different cellular models and situations. The immunofluorescent study of several cytoskeletal proteins was relevant in the evaluation of a therapy for osteoarthritis, in case of alkaptonuria and in testing the efficacy of docetaxel in neuroblastoma cancer cells leading to apoptosis. A relevant part of our experience focus on the study of cytoskeleton in seminiferous epithelium and spermatozoa, identifying alterations affecting blood-testis barrier after a silver nanoparticle treatment, chromosomal segregation in case of varicocele, sperm motility and diagnosing systematic sperm defects as “Primary ciliary dyskinesia” and “Dysplasia… More >

ANA LUCÍA DE PAUL^*, MÓNICA BONATERRA^*, CLAUDIA GABRIELA PELLIZAS^**, AGUSTÍN AOKI^*, ALICIA INÉS TORRES^*

BIOCELL, Vol.26, No.1, pp. 49-60, 2002, DOI:10.32604/biocell.2002.26.049

Abstract In the present study the in vivo and in vitro effects of GHRP-5 on the PRL-releasing activity in correlation with the morphological changes of lactotroph cells and their transcriptional activity were evaluated. The in vitro treatment (12 µg/100g BW/day for 3 days) of male rats with GHRP-5 does not induce any significant changes in serum PRL levels. In contrast, the addition of GHRP-5 to pituitary cell cultures increased significantly the release of PRL. This effect is enhanced in cell cultures of enriched lactotrophs, increasing significantly the secretion of PRL, the concentrations of which were 50% higher than that of untreated… More >