Hang Zhang^1,#, Meng-Yuan Chu^1,#, Guohui Lv¹, You-Jie Li¹, Xuhang Liu², Fei Jiao^1,*, Yun-Fei Yan^1,*

BIOCELL, Vol.50, No.1, 2026, DOI:10.32604/biocell.2025.068322 - 23 January 2026

Abstract Objectives: Non-small cell lung cancer (NSCLC) remains a leading cause of cancer-related mortality, with limited understanding of lncRNA-driven mechanisms in tumor progression. This study aimed to identify differentially expressed lncRNAs in NSCLC tissues and elucidate the functional role of the significantly upregulated RP3-340N1.2 in promoting malignancy. Methods: RNA sequencing was used to screen dysregulated lncRNAs. RP3-340N1.2 was functionally characterized via gain/loss-of-function assays in NSCLC cells, assessing proliferation, migration, and macrophage polarization. Mechanisms of interleukin 6 (IL-6) regulation were explored using cytokine profiling, Actinomycin D assays, and RNA Immunoprecipitation (RIP) assays to study RP3-340N1.2 interactions with… More >

NURAN BEDOLLA^#, HAO WU^#, LINYU LIU, XUETING LIU, YANLI REN^*

Oncology Research, Vol.33, No.6, pp. 1473-1484, 2025, DOI:10.32604/or.2025.060151 - 29 May 2025

Abstract Background: Gallic acid (GA), a plant-derived polyphenol, possesses diverse biological functions such as reducing inflammation and against tumors. Currently, the influence of GA on the resistance of esophageal squamous cell carcinoma (ESCC) cells to cisplatin (DDP) is not well understood. Methods: Cell counting kit-8 assay examined how GA affected KYSE30 and TE-1 cell viability. 5-Ethynyl-2′-deoxyuridine and TdT-mediated dUTP Nick-End labeling staining detected cell proliferation and apoptosis. Clone formation assay, flow cytometry, Carboxyfluorescein diacetate succinimidyl ester fluorescent probes, and Transwell assay determined cell biological properties, and 2′,7′-Dichlorofluorescin diacetate (DCFH-DA) fluorescent probes detected oxidative stress levels. Signal… More > Graphic Abstract

Francisco Javier Alarcón-Aguilar, Israel Domínguez de la Mora, Angeles Fortis-Barrera

European Cytokine Network, Vol.35, No.4, pp. 48-55, 2024, DOI:10.1684/ecn.2024.0499

Abstract Interleukin-6 (IL-6) is a cytokine with pleiotropic effects that plays a significant role in the transition from the innate immune response to adaptive response. IL-6 is of interest due to its proinflammatory action, however, it also exhibits anti-inflammatory effects, supporting metabolism and suppressing associated diseases, such as obesity, diabetes mellitus and metabolic syndrome. The IL-6 receptor (IL-6R) is a type I transmembrane glycoprotein in the plasma membrane of only some cell types, such as macrophages, neutrophils, hepatocytes, and T cells. The function of IL-6R requires another transmembrane glycoprotein of 130 kDa (gp130) which, in contrast… More >

YUAN YIN¹, ZHENGYIN WANG¹, YUJIE HU¹, JIA WANG¹, YI WANG^2,*, QUN LU^1,*

Oncology Research, Vol.32, No.12, pp. 1881-1890, 2024, DOI:10.32604/or.2024.048007 - 13 November 2024

Abstract Background: Caffeic acid (CA) is considered a promising phytochemical that has inhibited numerous cancer cell proliferation. Therefore, it is gaining increasing attention due to its safe and pharmacological applications. In this study, we investigated the role of CA in inhibiting the Interleukin-6 (IL-6)/Janus kinase (JAK)/Signal transducer and activator of transcription-3 (STAT-3) mediated suppression of the proliferation signaling in human prostate cancer cells. Materials and Methods: The role of CA in proliferation and colony formation abilities was studied using 3-[4,5-dimethylthiazol-2-yl]-2,5 diphenyl tetrazolium bromide (MTT) assay and colony formation assays. Tumour cell death and cell cycle arrest were… More >

Liling Chen¹, Xinyuan Chen²

European Cytokine Network, Vol.34, No.3, pp. 28-37, 2023, DOI:10.1684/ecn.2023.0488

Abstract Background: Relatively little is known about the relationship between Th1/Th2 cytokines and calculus cholecystitis (CC). The purpose of this study was to investigate the correlation between serum Th1 and Th2 cytokine expression and CC, including both acute and chronic cases. Methods: In total, 102 patients with chronic calculous cholecystitis (CCC), 64 patients with acute calculous cholecystitis (ACC), and 55 healthy controls (HCs) were recruited for the study. Serum concentration of Th1 (IL-2, TNF-α, IFN-γ) and Th2 cytokines (IL-4, IL-6, IL-10) was measured at admission and on the fifth day after cholecystectomy using flow cytometry. In addition,… More >

XIN LI^1,2,#, WENKAI JIANG^3,#, WANCHENG LI^3,#, SHI DONG³, YAN DU³, HUI ZHANG^1,*, WENCE ZHOU^1,3,*

Oncology Research, Vol.31, No.4, pp. 505-514, 2023, DOI:10.32604/or.2023.026959 - 25 June 2023

Abstract Background: The dilemma of pancreatic cancer treatment has become a global challenge. For this reason, effective, feasible, and new medical methods are currently much-needed. Betulinic acid (BA) has been valued as a potential therapy for pancreatic cancer. However, the mechanism by which BA exerts an inhibitory effect on the development of pancreatic cancer remains elusive. Methods: A rat model and two cell models of pancreatic cancer were established, and the effect of BA on pancreatic cancer was verified in vivo and in vitro by using MTT, Transwell, flow cytometry, RT-PCR, Elisa and immunohistochemistry. At the same time,… More > Graphic Abstract

Abdossaleh Zar¹, Fatemeh Ahmadi¹, Forouzan Karimi^2,*, Mozhgan Ahmadi³, Roger Ramsbottom⁴

Molecular & Cellular Biomechanics, Vol.19, No.1, pp. 51-59, 2022, DOI:10.32604/mcb.2022.018345 - 12 January 2022

Abstract The effect of resistance training and a herbal supplement on muscular signaling pathways are limited. We investigated the expression of IL-6, Gp130, JAK and STAT after resistance training, and Spirulina platensis supplementation in animal muscle. Thirty-two male Sprague Dawley rats (weight: 290 ± 20 g, and 9 weeks of age) were divided into four groups: control (CO; n = 8), Spirulina platensis supplementation (SP; n = 8), resistance exercise (RE; n = 8), and Spirulina platensis + resistance exercise (SP + RE; n = 8). The resistance exercise group trained five sessions each week for eight weeks. Spirulina… More >

SHUTING GU^1,2,#, JINGYI QIN^3,#, SAINAN GAO¹, ZHEN WANG^1,4, QI MENG^1,4, YAN LI^1,4, BING LU⁵, SONGLIN ZHOU^1,2,*, YUNZHAO XU^1,6,*

BIOCELL, Vol.46, No.3, pp. 689-697, 2022, DOI:10.32604/biocell.2022.016225 - 18 November 2021

Abstract Recently, abnormal expression of KIAA1199 has been detected in Epithelial Ovarian Cancer (EOC). However, the underlined anti-ovarian cancer mechanism of KIAA1199 remains to be enlightened. In our study, we performed to elucidate the effects of KIAA1199 on the advanced biological behavior of EOC cells through activation of the IL-6/STAT3 pathway. Confirmed by immunohistochemistry, KIAA1199 was highly expressed in ovarian borderline and malignant epithelial tumors. A retrospective analysis found that EOC patients with low expression of KIAA1199 had a significantly higher 5-year survival rate than those with high expression. Mechanistically, IL-6 was used to stimulate EOC More >

Maroua Chaouali^1,2, Mouna Ben Azaiez¹, Aymen Tezeghdenti¹, Besma Yacoubi-Oueslati², Ezzedine Ghazouani¹, Radhia Kochkar¹

European Cytokine Network, Vol.31, No.3, pp. 94-103, 2020, DOI:10.1684/ecn.2020.0450

Abstract Autoimmune hepatitis (AIH) is a chronic hepatitis of unknown etiology and several cytokines have been implicated in its pathogenesis and onset. Our objective was to determine the profile of pro and anti-inflammatory cytokines, including IL-1β, IL-6, IL-8, IL-23, IFN-γ, TNF-α, IL-10 in autoimmune hepatitis and their association with HLA gene polymorphisms. Serum cytokine levels were determined in 50 autoimmune hepatitis patients and one hundred fifty controls using chemiluminescence and ELISA techniques and HLA genotyping performed by PCR SSP. The levels of IL-6 (12 pg/mL vs. 5.5 pg/mL, p = 0.017), IL-8 (24.1 pg/mL vs. 7.8 More >

Ayse Ekin Kara¹, Gurhan Guney^1,2, Aytekin Tokmak¹, Gulnur Ozaksit¹

European Cytokine Network, Vol.30, No.1, pp. 29-33, 2019, DOI:10.1684/ecn.2019.0423

Abstract Objective: The aim of our study was to evaluate serum high-sensitivity C-Reactive Protein (hs-CRP), sialic acid (SA), and interleukin-6 (IL-6) levels in pregnancies complicated with preeclampsia (PE) and intrauterine growth restriction (IUGR) and to compare with healthy pregnancies. Materials and Methods: This study was conducted at a tertiary-level maternity hospital with 80 pregnant women. Fasting blood samples were taken from 44 consecutive women with pregnancies complicated by PE (n: 20) and IUGR (n: 24), and 36 were from normal pregnancies. Serum hs-CRP, SA, and IL-6 concentrations were measured in all participants. Results: Serum mean hs-CRP,… More >