WEN GE^1,2,#, YA LI^1,2,#, YUTING RUAN^1,2, NINGXIA WU^1,2, PEI MA^3,4, TONGPENG XU^3,4, YONGQIAN SHU^3,4, YINGWEI WANG^1,2, WEN QIU^1,2, CHENHUI ZHAO^3,4,*

Oncology Research, Vol.32, No.4, pp. 625-641, 2024, DOI:10.32604/or.2023.031053 - 20 March 2024

Abstract The cancer cell metastasis is a major death reason for patients with non-small cell lung cancer (NSCLC). Although researchers have disclosed that interleukin 17 (IL-17) can increase matrix metalloproteinases (MMPs) induction causing NSCLC cell metastasis, the underlying mechanism remains unclear. In the study, we found that IL-17 receptor A (IL-17RA), p300, p-STAT3, Ack-STAT3, and MMP19 were up-regulated both in NSCLC tissues and NSCLC cells stimulated with IL-17. p300, STAT3 and MMP19 overexpression or knockdown could raise or reduce IL-17-induced p-STAT3, Ack-STAT3 and MMP19 level as well as the cell migration and invasion. Mechanism investigation revealed… More > Graphic Abstract

Esther Mun Huieh Thou¹, Quok Cheong Choo², Choy Hoong Chew¹

European Cytokine Network, Vol.31, No.2, pp. 59-67, 2020, DOI:10.1684/ecn.2020.0446

Abstract Atherosclerosis is initiated when lipoproteins are trapped by proteoglycans in the arterial intima. Macrophages play a vital role in this disease, especially in the formation of foam cells and the regulation of pro-inflammatory responses. They also participate in plaque stabilization through the secretion of matrix metalloproteinases. Studies have reported the role of ADAMTS proteases in osteoarthritis and atherosclerotic lesions. In the present study, we have studied the effect of interleukin-17A (IL-17A) on the expression of ADAMTS-5 in the macrophage cell line THP-1. The results show that the mRNA and protein expression levels of ADAMTS-5 were More >

Chuanwei Xu¹, Renju Ding², Chuanping Xu^3,*

Oncologie, Vol.22, No.1, pp. 43-51, 2020, DOI:10.32604/oncologie.2020.012445

Abstract To analyze the effects of different surgical procedures for the treatment of thyroid cancer on the expression levels of serum interleukin-17 (IL-17), IL-35, soluble interleukin-2 receptor (SIL-2R), and the prognostic factors. Seventy-eight patients with differentiated thyroid cancer were selected and grouped as control group (CG) (n = 39, underwent subtotal thyroidectomy) and observation group (OG) (n = 39, underwent total thyroidectomy). The serum IL-17, IL-35, and SIL- 2R expression levels; the incidence of complications; and the differentiated thyroid carcinoma (DTC) relapse rate were compared between the two groups. The serum IL-17 and SIL-2R levels were… More >

Roba M. Talaat¹, Hiba Sibaii², Iman H. Bassyouni³, Amany El-Wakkad⁴

European Cytokine Network, Vol.30, No.1, pp. 15-22, 2019, DOI:10.1684/ecn.2019.0421

Abstract There are a limited number of studies that report the polarization of the immune system toward the production of T helper 1 (Th1), Th2, or Th17-type cytokines in patients with Behçet’s disease (BD). Here, we aimed to detect the presence of various cytokines in serum samples of Egyptian BD patients and to determine the correlation between their production levels and clinical manifestations. To that aim, serum levels of IFN-γ, IL-10, IL-6, and IL-17 measured by ELISA were determined in BD patients with active or inactive disease to evaluate their clinical relevance. The results of the… More >

Takehiro Hasegawa^1,2, Hitoshi Uga¹, Akio Mori³, Hirokazu Kurata^1,2

European Cytokine Network, Vol.28, No.1, pp. 8-18, 2017, DOI:10.1684/ecn.2017.0390

Abstract Asthma is a syndrome of chronic bronchial inflammation and airway remodelling. Initially, asthma has been categorized into atopic and nonatopic types, based on antigen-specific IgE levels. Moreover, recently, asthma has been classified into different endotypes based on its pathophysiology, leading to the selection of the most optimal and effective therapies. Although T helper cell type 2 (Th2) cytokines were proven to play critical roles in atopic asthma, IL-17A has been reported to be involved in severe refractory asthma. Patients and methods: In this study, we measured the levels of 24 cytokines/chemokines in the sera of… More >

José L. Aparicio¹, Macarena Ottobre¹, Maite Duhalde Vega¹, Jean-Paul Coutelier², Jacques Van Snick³, Lilia A. Retegui¹

European Cytokine Network, Vol.28, No.3, pp. 111-119, 2017, DOI:10.1684/ecn.2017.0399

Abstract Mice infected with mouse hepatitis virus A59 (MHV-A59) develop hepatitis and autoantibodies (autoAb) to liver and kidney fumarylacetoacetate hydrolase (FAH), a fact closely related to the release of alarmins such as uric acid and/or high-mobility group box protein 1 (HMGB1). We studied the effect of neutralizing monoclonal antibodies (MAb) against IL-17A in our model of mouseMHV-A59-infection.MAb anti-IL-17F and anti-IFNγ were used to complement the study. Results showed that transaminase levels markedly decreased in MHV-A59- infected mice treated with MAb anti-IL-17A whereas plasmatic Ig concentration sharply increased. Conversely, MAb anti-IL-17F enhanced transaminase liberation and did not More >

Dorota Mańkowska-Wierzbicka, Ewelina Swora-Cwynar, Barbara Poniedziałek, Zygmunt Adamski, Agnieszka Dobrowolska, Jacek Karczewski

European Cytokine Network, Vol.26, No.2, pp. 26-37, 2015, DOI:10.1684/ecn.2015.0363

Abstract Introduction: This study aimed to compare the accuracy of selected laboratory markers in assessing disease activity in patients with ulcerative colitis (UC). The analysis included serum IL-2, IL-4, IL-6, IL-10, IL-17, TNF-α, IFN-γ, hsCRP, peripheral regulatory T cells, as well as fecal calprotectin and lactoferrin. Patients and methods: A group of 45 adults with UC was enrolled in the study. Disease activity was assessed using the Mayo endoscopic index, while for clinical activity scoring, the Clinical Activity Index (CAI) was used. Concentrations of markers investigated were estimated by means of flow cytometry and enzyme-linked immunosorbent… More >

Hongtao Ren^*, Zhongwei Wang^*, Shuqun Zhang^*, Hongbing Ma^*, Yali Wang^*, Lijun Jia^*, Yiming Li^†

Oncology Research, Vol.23, No.5, pp. 249-256, 2015, DOI:10.3727/096504016X14562725373716

Abstract Interleukin-17A (IL-17A) plays a significant role in many inflammatory diseases and cancers. The aim of this study is to investigate the effect of IL-17A on the invasiveness of colorectal cancer. In the study, we found that IL-17A could promote the migration and invasion of colorectal cancer cells. Furthermore, after being treated with IL-17A, the expression and activity of matrix metalloproteinase 2 (MMP-2) and MMP-9 were upregulated. Moreover, the nuclear/overall fractions and DNA-binding activity of p65 and p50 were dramatically elevated by IL-17A. Pretreatment with a nuclear factor-kB (NF-kB) inhibitor (PDTC) or PI3K/AKT inhibitor (LY294002) was More >

Anargyros Kouris¹, Aikaterini Pistiki², Alexandros Katoulis¹, Marianna Georgitsi², Sofia Giatrakou¹, Evangelia Papadavid¹, Mihai G. Netea³, Nikolaos Stavrianeas¹, Evangelos J. Giamarellos-Bourboulis^2,3

European Cytokine Network, Vol.25, No.4, pp. 63-68, 2014, DOI:10.1684/ecn.2014.0358

Abstract Background: Psoriasis is one of the most common, immune-mediated, chronic inflammatory skin diseases. Proinflammatory cytokines play an important pathogenetic role at a local level. Objective: To assess whether the proinflammatory cytokines IL-1β, IL-6, IL-17, IL-22 and TNF-α are released systemically during psoriasis. Methods: Peripheral blood mononuclear cells (PBMCs) were isolated from 30 patients with psoriasis and 30 healthy volunteers. Cytokine production was assessed in supernatants using an enzyme immunoassay after stimulation of PBMCs with microbial stimuli. In addition, flow cytometry was used to determine the subsets of monocytes involved and the intracellular TNF-α production in More >

Ewa Robak¹, Lilianna Kulczycka-Siennicka¹, Zofia Gerlicz¹, Monika Kierstan¹, Anna Korycka-Wolowiec², Anna Sysa-Jedrzejowska¹

European Cytokine Network, Vol.24, No.1, pp. 60-68, 2013, DOI:10.1684/ecn.2013.0330

Abstract Systemic lupus erythematosus (SLE) is an autoimmune disease of multifactorial pathoaetiology. Different organs and blood vessels may be affected by chronic inflammation. A direct cause of the disease has not yet been found, so research is being carried out to this effect. The role of the recently identified helper T lymphocyte CD4+, described as Th17, and its dependent cytokines have been of particular interest. The aim of the study was to evaluate IL-17A, IL-17B, IL-17F and IL-23 in 60 SLE patients and 26 age-matched, healthy volunteers and also to investigate the correlation between levels of… More >