JING WANG^1,#, JUNFENG XU^2,#, YINGRAN YANG¹, YOUZHENG QIU¹, SHANSHAN ZHANG³, NING WANG^1,3,*

BIOCELL, Vol.48, No.9, pp. 1343-1353, 2024, DOI:10.32604/biocell.2024.051673 - 04 September 2024

Abstract Objective: This study aimed to elucidate the influence of IFN-gamma (IFN-γ) in neuroblastoma (NB) cells and reveal its potential underlying molecular mechanism. Methods: The Cell Counting Kit-8, Transwell apparatus, and flow cytometry were employed to assess cellular viability, migratory capacity, invasive potential, and apoptotic rates, respectively. RNA-seq combined with bioinformatics analysis revealed differentially expressed genes (DEGs) and their possible biological functions. Protein levels were determined by western blot analysis. Results: IFN-γ treatment resulted in diminished cell viability, mitigated migratory and invasive capabilities, and augmented apoptotic activity in the SK-N-BE (2) cell line, whereas it exhibited the… More >

Fatemeh Bayati^1,*, Maryam Valadi^1,*, Armin Ahmadi^1,2, Farangis Najaf¹, Bita Ansaripour¹, Ehsan Sharif-Paghaleh^1,3

European Cytokine Network, Vol.33, No.3, pp. 70-78, 2022, DOI:10.1684/ecn.2022.0482

Abstract Although mesenchymal stem cells (MSCs) have exhibited promising immunomodulatory potential in preclinical studies, clinical studies have revealed variable results. These results often depend on environmental cues. Pre-conditioning MSCs with cytokines is one of the methods used to enhance their immunomodulatory effects. In this study, we harvested adipose-derived MSCs from mice and cultured them with different doses of the cytokine, IFN-γ, and the corticosteroid drug, dexamethasone, in order to investigate their effects on MSC immunosuppressive function. We found the co-culture or supernatant of MSCs, pre-conditioned with IFN-γ, together with spleen mononuclear cells resulted in a significant More >

Maroua Chaouali^1,2, Mouna Ben Azaiez¹, Aymen Tezeghdenti¹, Besma Yacoubi-Oueslati², Ezzedine Ghazouani¹, Radhia Kochkar¹

European Cytokine Network, Vol.31, No.3, pp. 94-103, 2020, DOI:10.1684/ecn.2020.0450

Abstract Autoimmune hepatitis (AIH) is a chronic hepatitis of unknown etiology and several cytokines have been implicated in its pathogenesis and onset. Our objective was to determine the profile of pro and anti-inflammatory cytokines, including IL-1β, IL-6, IL-8, IL-23, IFN-γ, TNF-α, IL-10 in autoimmune hepatitis and their association with HLA gene polymorphisms. Serum cytokine levels were determined in 50 autoimmune hepatitis patients and one hundred fifty controls using chemiluminescence and ELISA techniques and HLA genotyping performed by PCR SSP. The levels of IL-6 (12 pg/mL vs. 5.5 pg/mL, p = 0.017), IL-8 (24.1 pg/mL vs. 7.8 More >

Roba M. Talaat¹, Hiba Sibaii², Iman H. Bassyouni³, Amany El-Wakkad⁴

European Cytokine Network, Vol.30, No.1, pp. 15-22, 2019, DOI:10.1684/ecn.2019.0421

Abstract There are a limited number of studies that report the polarization of the immune system toward the production of T helper 1 (Th1), Th2, or Th17-type cytokines in patients with Behçet’s disease (BD). Here, we aimed to detect the presence of various cytokines in serum samples of Egyptian BD patients and to determine the correlation between their production levels and clinical manifestations. To that aim, serum levels of IFN-γ, IL-10, IL-6, and IL-17 measured by ELISA were determined in BD patients with active or inactive disease to evaluate their clinical relevance. The results of the… More >

Carlos Erik Medina Santos¹, Carmen Nathaly López Hurtado¹, Bruno Rivas Santiago¹, Roberto Gonzalez-Amaro³, Yolanda Guadalupe Cataño Cañizales¹, Margarita de la Luz Martínez Fierro², José Antonio Enciso-Moreno¹, Mariana Haydee García Hernández¹

European Cytokine Network, Vol.27, No.3, pp. 68-74, 2016, DOI:10.1684/ecn.2016.0379

Abstract The aim of this study was to evaluate the effects of the LL-37, HNP-1 and HBD2/3 peptides on cytokine and MMP production in human polymorphonuclear cells, mononuclear cells and chondrocytes. The levels of cytokines in supernatants from mononuclear and polymorphonuclear cell cultures were measured with a cytometric bead array by flow cytometry. Likewise, the levels of metalloproteinase/MMP-1, 3, and 13 were measured in supernatants from chondrocyte cultures using an ELISA. The expression of RANKL on lymphocytes was analyzed by flow cytometry. We observed increased levels of TNF-α, IL-6 and IL-10 in mononuclear and polymorphonuclear cell… More >

Hubertus Himmerich^1,a, Johannes Fischer^2,a, Katrin Bauer³, Kenneth C. Kirkby⁴, Ulrich Sack³, Ute Krügel²

European Cytokine Network, Vol.24, No.2, pp. 97-103, 2013, DOI:10.1684/ecn.2013.0338

Abstract Stress-induced cytokine changes may be the link between stress and the pathogenesis of psychiatric disorders such as depression, and organic diseases such as infections, autoimmune diseases and cancer. We tested the effect of stress on interleukin (IL)-2, IL-4, IL-6, IL-10, IL-22, tumour necrosis factor (TNF)-α and interferon (IFN)-γ serum levels in male Wistar rats. Rats underwent either acute stress by forced swimming (N = 8), chronic restraint stress (N = 8), or were not subjected to any stress (N = 8). IL-2 serum levels were significantly higher in forced swimming, but not in restraint stress… More >

Mahmoud Nateghi Rostami¹, Masoumeh Douraghi², Akram Miramin Mohammadi³, Bahram Nikmanesh⁴

European Cytokine Network, Vol.23, No.2, pp. 64-67, 2012, DOI:10.1684/ecn.2012.0307

Abstract There are reports showing that pro-inflammatory cytokines are dysregulated in patients with Down’s syndrome (DS). However, most of these reports concern adults. We analyzed cytokine levels in serum samples from children with DS, and compared them with samples from intellectually disabled (ID), and healthy, control children.Blood samples were collected from 24 DS, 24 age-/sex-matched ID, and 24 age-/sex-matched healthy, control children. Serum levels of the cytokines IL-5, IL-10, IL-13, IFN-γ, and TNF-α were measured using a sandwich ELISA method, .The age range of the children was 1-15 years, with a mean ± SD of 5.75… More >

Johannes Nemeth^1,3, Heide-Maria Winkler¹, Franz Karlhofer², Nicole Selenko-Gebauer², Wolfgang Graninger¹, Stefan Winkler¹

European Cytokine Network, Vol.21, No.1, pp. 34-39, 2010, DOI:10.1684/ecn.2009.0182

Abstract Patients treated with tumor necrosis factor (TNF)-α-antagonizing medication are at increased risk of developing active tuberculosis (TB), brought about mainly by reactivation of latent infection. Thus, screening for latent TB infection (LTBI) prior to administration of anti-TNF-α-therapy is required. For a long time, the tuberculin skin test (TST) was the only means of diagnosing LTBI, however, interferon-gamma-release assays (IGRAs), are promising new tools. Fifty two patients with dermatological disorders were included prior to imple-mentation of anti-TNF-α therapy. Mycobacterium tuberculosis (MTB)-specific cytokine production, including interferon (IFN)-γ, TNF-α, interleukin (IL)-2 and IL-10, was measured in CD4⁺ and CD8⁺ T… More >