Hong Li*1,
Yaning Tian*1,
Xiang Li*, Bin Wang†, Dongzhi Zhai*, Yingying Bai*,
Changhu Dong*, Xu Chao*‡
Oncology Research, Vol.27, No.6, pp. 673-680, 2019, DOI:10.3727/096504018X15426261956343
Abstract IARS2 encodes mitochondrial isoleucine-tRNA synthetase, which mutation may cause multiple diseases.
However, the biological function of IARS2 on acute myeloid leukemia (AML) has not yet been identified.
In the present study, qRT-PCR was used to determine the expression of IARS2 in K562, THP1, and HL-60
leukemia cells. Additionally the mRNA levels of IARS2 in CD34 cells and AML cells obtained from patients
were detected by qRT-PCR. IARS2-shRNA lentiviral vector was established and used to infect acute myeloid
leukemia HL-60 cells. qRT-PCR and Western blot analysis were employed to assess the knockdown effect of
IARS2. The… More >
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