WENLI JIANG^1,#, JUNYI SHEN^1,#, XIAOQIANG DU^1,#, YAN QIU¹, JIAN ZHONG¹, ZHI OUYANG¹, BINGMEI M. FU², YE ZENG^1,*

BIOCELL, Vol.47, No.10, pp. 2255-2263, 2023, DOI:10.32604/biocell.2023.030880 - 08 November 2023

Abstract Background: Anisodine hydrobromide (AT3), an anti-cholinergic agent, could be delivered to the brain across the blood-brain barrier and has been used clinically for the treatment of cerebral ischemia/reperfusion injury. Endothelial dysfunction can be caused by hypoxia/reoxygenation (H/R) via oxidative stress and metabolic alterations. The present study investigated whether AT3 regulates the production of nitric oxide (NO) and reactive oxygen species (ROS), and the HIF-1α pathway via regulation of muscarinic acetylcholine receptors (mAChRs) in brain microvascular endothelial cells after H/R exposure. Methods: Under H/R conditions, hCMEC/D3 cerebral microvascular endothelial cells were treated with AT3. Specific inhibitors… More > Graphic Abstract

Hong Liu^1,#,*, Luyao Ma^1,#, Jinghang Li^1,#, Bingqi Sun², Siqiang Zheng³, Yongfeng Shao^1,*

Congenital Heart Disease, Vol.16, No.5, pp. 443-455, 2021, DOI:10.32604/CHD.2021.015588 - 03 June 2021

Abstract Background: This study evaluated differential inflammatory response to cardiopulmonary bypass reoxygenation in tetralogy of Fallot repair. Methods: We performed a retrospective study at a cardiovascular center from 2012 to 2018, including 500 patients aged 1 week–18 years who received complete repair of tetralogy of Fallot. Patients were grouped according to tertiles of preoperative RVOT gradient on echocardiography into mild, moderate, and severe stenosis. We measured the highest perfusate oxygenation (PpO2) during aortic occlusion as independent variable. Primary outcome was systemic inflammatory response syndrome (SIRS) within 7 days postoperatively or the time of death or discharge. Results: Overall, rate… More >

Sanae Haga^*, Akira Kanno^†, Takeaki Ozawa^‡, Naoki Morita^§, Mami Asano^*¶,¶ and Michitaka Ozaki^*¶

Oncology Research, Vol.26, No.3, pp. 503-513, 2018, DOI:10.3727/096504017X15005102445191

Abstract Liver injury is often observed in various pathological conditions including posthepatectomy state and cancer chemotherapy. It occurs mainly as a consequence of the combined necrotic and apoptotic types of cell death. In order to study liver/hepatocyte injury by the necrotic type of cell death, we studied signal-regulated necrosis (necroptosis) by developing a new optic probe for detecting receptor-interacting protein kinase 1 (RIP)/RIP3 binding, an essential process for necroptosis induction. In the mouse hepatocyte cell line, TIB-73 cells, TNF-a/cycloheximide (T/C) induced RIP1/3 binding only when caspase activity was suppressed by the caspase-specific inhibitor z-VAD-fmk (zVAD). T/C/zVAD-induced… More >