Yanhuan Lin^1,#, Jiaxin Feng^2,#, Hao Fang², Wei Huang², Kanglie Guo², Xiyan Liu², Shuqi Wang², Xiaojuan Liu^2,*

Phyton-International Journal of Experimental Botany, Vol.93, No.10, pp. 2583-2596, 2024, DOI:10.32604/phyton.2024.055616 - 30 October 2024

Abstract Heat shock transcription factor (HSF) are essential regulators of heat shock protein (HSP) gene expression in plants and algae, contributing to their resilience against biotic and abiotic stresses. However, the localization, structure, phylogenetic relationship, and characteristics of PtHSF genes in microalgae, especially in diatom Phaeodactylum tricornutum, remain largely unexplored. This study presents a comprehensive analysis of the PtHSF gene family in P. tricornutum. A genome-wide analysis identified 68 PtHSF genes, which were classified into two distinct subfamilies: traditional and untraditional. Motif and structure analyses revealed evidence of multiple duplication events within the PtHSF gene family. Expression profiling revealed diurnal… More >

CHEN WANG, CHAOFAN HE, YUANYUAN GAO, KAIXIAN WANG, MENG LIANG^*

BIOCELL, Vol.47, No.1, pp. 155-164, 2023, DOI:10.32604/biocell.2023.024657 - 26 September 2022

Abstract Heat stress is an important influence on the male reproductive organs. Therefore, the effects of heat stress on genes or pathways related to the reproductive system of male mice were experimentally explored in this paper to further determine the effects of heat stimulation on mammals. Herein, models of heat-exposed mouse testicular tissue and heat-excited cells were successfully established. Many scorched vesicles were found after heat excitation of testis supporting cells, testicular mesenchymal (TM4) cells. Western blot, in situ terminal deoxynucleotide transferase dUTP Nick end labeling (TUNEL) and transmission electron microscopy showed that membrane rupture, mitochondrial damage More >

Yuzhe Zhang^1,#, Guanglong Li^1,#, Shaowang Hu¹, Jinfeng Liu¹, Yushi Jiang¹, Siyan Liu^2,*, Shuyan Guan^2,*, Jing Qu², Dan Yao¹, Andi Shi¹, Yixuan Liu¹

Phyton-International Journal of Experimental Botany, Vol.91, No.6, pp. 1183-1198, 2022, DOI:10.32604/phyton.2022.018853 - 14 February 2022

Abstract Soybean (Glycine max (L.) Merr.) is an important cultivated crop, which requires much water during its growth, and drought seriously affects soybean yields. Studies have shown that the expression of small heat shock proteins can enhance drought resistance, cold resistance and salt resistance of plants. In this experiment, soybean GmHsps_p23-like gene was successfully cloned by RT-PCR, the protein encoded by the GmHsps_p23-like gene was subjected to bioinformatics analysis, and the pCAMBIA3301-GmHsps_p23-like overexpression vector and pCBSG015-GmHsps_p23-like gene editing vector were constructed. Agrobacterium-mediated method was used to transform soybeans to obtain positive plants. RT-PCR detection, rehydration experiment and drought resistance… More >

Muslum S. Inal^1,2, Dilek Unal^1,*, Bengu Turkyilmaz Unal^3,*, Munir Ozturk⁴

Phyton-International Journal of Experimental Botany, Vol.91, No.3, pp. 583-598, 2022, DOI:10.32604/phyton.2022.018223 - 26 October 2021

Abstract Putrescine is reported to be necessary for cold acclimation under low-temperature stress. In this study, the effect of low-temperature on some physiological and biochemical parameters has been investigated using the green algae Chlamydomonas reinhardtii. The lipid peroxidation rate, amount of Rubisco protein, activities of antioxidant enzymes and gene expression of polyamine biosynthesis (odc2, and spd1), heat shock proteins (hsp70c, hsp90a, and hsp90c), and PSII repair mechanisms (psba, rep27, and tba1) were determined to understand the low-temperature response. Exogenous putrescine application significantly increased Rubisco protein concentration and catalase enzyme activities under low-temperature stress. Moreover, real-time RT-PCR results and gene expression analysis… More >

Zongqiang GAO¹, Xiong GUO², Junchang CHEN¹, Chen DUAN²

BIOCELL, Vol.43, No.2, pp. 99-102, 2019, DOI:10.32604/biocell.2019.06520

Abstract This study aimed to investigate the effect of hyaluronic acid (HA) on the expression of heat shock protein 70 (HSP70) in chondrocytes isolated from patients with osteoarthritis (OA) and Kashin-Beck disease (KBD). The chondrocytes were collected from OA and KBD patients, and chondrocytes isolated from patients of accident injuries were used as the control. The chondrocytes were treated with HA at different doses. HSP70 expression in chondrocytes at both mRNA and protein levels was tested by PCR and Western blot analysis. Compared with control, both mRNA and protein levels of HSP70 were higher in chondrocytes More >

Bui Thi Kim Ly^*, Hoang Thanh Chi^†‡

Oncology Research, Vol.26, No.8, pp. 1201-1205, 2018, DOI:10.3727/096504018X15154104709325

Abstract FMS-like tyrosine kinase-3 fragments from exon 14 to the end without any mutations or deletions have been reported to fuse to ETV6 (TEL) in a few cases of myeloid/lymphoid neoplasms with eosinophilia carrying a translocation t(12;13)(p13;q12). This fusion protein confers constitutive activation on the FLT3 fragment and induces factor-independent growth in transfected Ba/F3 cells, indicating that it is an oncoprotein. However, the mechanism controlling the stability of this oncoprotein is unknown. In this study, we focus on finding factors controlling the stability of ETV6/FLT3. We have shown that the stability of ETV6/FLT3 is regulated by… More >

Gangcan Li, Yanping Song, Yunjie Zhang, Hao Wang, Jia Xie

Oncology Research, Vol.24, No.2, pp. 109-116, 2016, DOI:10.3727/096504016X14611963142254

Abstract Acute myeloid leukemia (AML) is the most lethal hematological malignancy, and the occurrence of chemoresistance prevents the achievement of complete remission following the standard therapy. MicroRNAs have been extensively investigated as critical regulators of hematopoiesis and leukemogenesis, and they represent a promising strategy for AML therapy. In this study, we identified miR-34b as a novel regulator in myeloid proliferation and apoptosis of leukemic cells. We found that miR-34b was developmentally upregulated in plasma and myeloid cells of healthy subjects, while it was significantly reduced in blood samples of patients with AML and AML cell lines.… More >

Encieh Choghaei^*, Gholamreza Khamisipour^†, Mojtaba Falahati^*, Behrooz Naeimi^†, Majid Mossahebi-Mohammadi^‡, Rahim Tahmasebi^†, Mojtaba Hasanpour^†, Shakib Shamsian^†, Zahra Sadat Hashemi^§

Oncology Research, Vol.23, No.1-2, pp. 69-78, 2015, DOI:10.3727/096504015X14478843952906

Abstract Breast cancer is the most commonly occurring cancer among women. MicroRNAs as noncoding small RNA molecules play pivotal roles in cancer-related biological processes. Increased levels of microRNA-29a in the serum of breast cancer patients have been reported. Since heat shock proteins (HSPs) play important roles in cell events, the quantitative fluctuations in their cellular levels could be deemed as key indicators of how the exerted treatment alters cell behavior. In this regard, using an antisense small RNA, we attempted to investigate the effects of miR-29a knockdown on the expression of HSPs genes in the MCF-7… More >

B.C. Heng*, K.J. Vinoth*, K. Lu*, X. Deng*, Z. Ge*, B.H. Bay**, T. Cao*

BIOCELL, Vol.31, No.3, pp. 405-410, 2007, DOI:10.32604/biocell.2007.31.405

Abstract We investigated the effects of prolonged heat shock treatment on human embryonic stem cell (hESC) viability. The hESC viability steadily declined with longer exposure to heat shock treatment (43ºC). After 4 h of exposure to heat shock at 43ºC, only 56.2 ± 1.5% of cells were viable. Viability subsequently declined to 37.0 ± 3.3% and 3.5 ± 0.7% after 8 h and 16 h, respectively of heat shock treatment at 43ºC. Transmission electron micrographs showed that the morphology of the dead/dying cells after heat shock treatment was characteristic of cellular necrosis with an uncondensed chromatin… More >