DIMITRA P. VAGELI^1,2,*, PANAGIOTIS G. DOUKAS³, KERASIA GOUPOU², ANTONIOS D. BENOS², KYRIAKI ASTARA^2,4, KONSTANTINA ZACHAROULI², SOTIRIS SOTIRIOU⁵, MARIA IOANNOU²

Oncology Research, Vol.32, No.8, pp. 1239-1256, 2024, DOI:10.32604/or.2024.052130 - 17 July 2024

Abstract Glioblastoma multiforme (GBM) is an aggressive primary brain tumor characterized by extensive heterogeneity and vascular proliferation. Hypoxic conditions in the tissue microenvironment are considered a pivotal player leading tumor progression. Specifically, hypoxia is known to activate inducible factors, such as hypoxia-inducible factor 1alpha (HIF-1α), which in turn can stimulate tumor neo-angiogenesis through activation of various downward mediators, such as the vascular endothelial growth factor (VEGF). Here, we aimed to explore the role of HIF-1α/VEGF immunophenotypes alone and in combination with other prognostic markers or clinical and image analysis data, as potential biomarkers of GBM prognosis… More >

YIWEN ZHU¹, LIU YANG¹, YING YU¹, YING XIONG², PING XIAO², XIAO FU², XIN LUO^2,*

Oncology Research, Vol.32, No.5, pp. 899-910, 2024, DOI:10.32604/or.2023.042604 - 23 April 2024

Abstract Osteosarcoma is a very serious primary bone cancer with a high death rate and a dismal prognosis. Since there is no permanent therapy for this condition, it is necessary to develop a cure. Therefore, this investigation was carried out to assess the impacts and biological functions of hydroxysafflor yellow A (HYSA) in osteosarcoma cell lines (MG63). In this investigational study, MG63 cells were utilized. Microarray experiments, quantitative polymerase chain reaction (qPCR), immunofluorescent staining, extracellular acidification rate (ECAR), oxygen consumption rate (OCR), glucose consumption, lactate production, and ATP levels, proliferation assay, 5-Ethynyl-2′-deoxyuridine (EDU) staining, and Western… More >

CHENYANG CHEN^1,*, JUAN WEN¹, WEI HUANG¹, JIANG LI^2,*

BIOCELL, Vol.47, No.11, pp. 2423-2433, 2023, DOI:10.32604/biocell.2023.044459 - 27 November 2023

Abstract Background: Pulmonary arterial hypertension (PAH) is a chronic and progressive disease that is strongly associated with dysregulation of glucose metabolism. Alterations in nuclear receptor subfamily 4 group A member 1 (NR4A1) activity alter the outcome of PAH. This study aimed to investigate the effects of NR4A1 on glycolysis in PAH and its underlying mechanisms. Methods: This study included twenty healthy volunteers and twenty-three PAH patients, and plasma samples were collected from the participants. To mimic the conditions of PAH in vitro, a hypoxia-induced model of pulmonary artery smooth muscle cell (PASMC) model was established. The proliferation… More > Graphic Abstract

RUICONG GUAN^1,3,#, KUAN ZENG^1,#, MINNAN GAO¹, JIANFEN LI¹, HUIQI JIANG¹, LU ZHANG¹, JINGWEN LI¹, BIN ZHANG¹, YUQIANG LIU¹, ZHUXUAN LIU¹, DIAN WANG¹, YANQI YANG^1,2,*

BIOCELL, Vol.46, No.11, pp. 2455-2463, 2022, DOI:10.32604/biocell.2022.021661 - 07 July 2022

Abstract Bone marrow mesenchymal stem cell (MSC)-based therapy is a novel candidate for heart repair. But ischemia-reperfusion injury leads to low viability of MSC. Dexmedetomidine (Dex) has been found to protect neurons against ischemia-reperfusion injury. It remains unknown if Dex could increase the viability of MSCs under ischemia. The present study is to observe the potential protective effect of Dex on MSCs under ischemia and its underlying mechanisms. Specific mRNAs related to myocardial ischemia in the GEO database were selected from the mRNA profiles assessed in a previous study using microarray. The most dysregulated mRNAs of… More >

YAPING WANG^1,2,#,*; XIAOHONG XUE^2,#; ZHEN ZHAO^1,#; XIAOLIN LI²; ZHIYONG ZHU¹

BIOCELL, Vol.46, No.7, pp. 1651-1659, 2022, DOI:10.32604/biocell.2022.018559 - 17 March 2022

Abstract The pathogenesis of high altitude-related gastric mucosal injury remains poorly understood, this study aimed to investigate the role of autophagy in hypoxia-induced apoptosis of rat gastric mucosal cells. Rats were randomized into four groups which were maintained at an altitude of 400 m (P) or received no treatment (H), autophagy inducer rapamycin (H+AI) or autophagy inhibitor 3-MA (H+AB) at an altitude of 4,300 m for 1, 7, 14 and 21 days, respectively, and the morphology, ultrastructure, autophagy, and apoptosis of gastric mucosal tissues were examined. Gastric mucosal epithelial cells CC-R039 were cultured under conditions of… More >

RUONAN GU^1,2, WENJING GUO², WENYANG WANG¹, GUIHUAN LI¹, XIAOJU LAI¹, ZHIBIN HUANG¹, WANLU ZHAO¹, ZHICONG WU¹, HUA CHEN¹, WENYANG LUO¹, FANGYIN ZENG^3,*, FAN DENG^1,*

BIOCELL, Vol.45, No.6, pp. 1585-1599, 2021, DOI:10.32604/biocell.2021.017016 - 01 September 2021

Abstract Propofol, a common intravenous anesthetic used in clinical, has been shown to regulate cells proliferation, inflammation, angiogenesis and metastasis as well as exerting anti-cancer effect in several different types of cancer. However, the functions and mechanisms of propofol in lactate secretion and invasion of cancer cells remain unknown. The primary aim of this study was to investigate the role of propofol in cells migration and invasion of colon cancer (CRC). Scratches assay, Transwell assay were used to detect colon cells migration and invasion. Real-time Quantitative Polymerase Chain Reaction (RT-qPCR), Western blotting were utilized to detect… More >

Xinlu Liu, Xiaodong Tan, Peng Liu, Yunhao Wu, Songying Qian, Xiaobo Zhang

Oncology Research, Vol.26, No.7, pp. 1123-1131, 2018, DOI:10.3727/096504018X15166223632406

Abstract Pancreatic ductal adenocarcinoma (PDAC) is one of the most aggressive tumors known, with an overall 5-year survival rate of less than 6% due to early local invasion and distant metastasis. Exploring suitable therapeutic targets associated with invasion and metastasis is required for improving the prognosis of PDAC. In this study, we investigated the role of the glycolytic enzyme phosphoglycerate mutase 1 (PGAM1) in PDAC. PGAM1 expression was examined in tissue samples of 54 PDAC patients using immunohistochemistry, and the correlation between clinicopathological expression and PGAM1 expression was determined. A survival curve was generated using the… More >

Qianren Xiao^*1, Lu Huang^†1, Zhongzu Zhang^‡1, Xiang Chen^*, Jiaquan Luo^*, Zhanmin Zhang^§, Shaoqing Chen^§, Yong Shu^*, Zhimin Han^*, Kai Cao^*

Oncology Research, Vol.25, No.2, pp. 267-275, 2017, DOI:10.3727/096504016X14732510786564

Abstract miRNAs play a pivotal role in the development and progression of osteosarcoma (OS). Previous studies indicated that miR-140 acts as a tumor suppressor in many cancers. However, its accurate expression and exact function in OS cells remain unknown. Herein, we demonstrated the lower expression of miR-140 in 40 paired OS tissues. Restoring miR-140 expression in OS cells had a marked effect on inhibiting cell proliferation and invasion, inducing cell apoptosis in vitro, and suppressing tumor growth in vivo. Moreover, a bioinformatics prediction indicated that the histone deacetylase 4 (HDAC4) is a target gene of miR-140 and More >

Qun Zhao, Bi-Bo Tan, Yong Li, Li-Qiao Fan, Pei-Gang Yang, Yuan Tian

Oncology Research, Vol.23, No.3, pp. 129-136, 2015, DOI:10.3727/096504015X14500513118029

Abstract In this study, the effects of hypoxia-inducible factor-1α (HIF-1α) on gastric carcinoma (GC) drug resistance through apoptosis-related genes are investigated. First, HIF-1α-specific siRNA was synthetized and transfected into drug-resistant GC cell line OCUM-2MD3/L-OHP. Then MTT assay was applied to test the inhibition rate of GC cells by 5-fluorouracil (5-FU) and oxaliplatin (L-OHP). After that, flow cytometry (FCM) was applied to measure apoptosis rate. qPCR and Western blot assay were employed to detect HIF-1α and apoptosis-related genes. Results showed that HIF-1α in OCUM-2MD3/L-OHP cells was higher than that in OCUM-2MD3 and gastric epithelial cells. After HIF-1α-siRNA More >

Fei Liu^*1, Bihua Lin^*1, Xin Liu^*, Wenzhang Zhang^*, Erying Zhang^*, Liang Hu^*, Yuefan Ma^*, Xiangyong Li^*, Xudong Tang^*†

Oncology Research, Vol.23, No.3, pp. 109-118, 2015, DOI:10.3727/096504015X14496932933610

Abstract Extracellular signal-regulated kinase (ERK)1/2 signaling pathway plays a critical role in regulating tumor angiogenesis. Our previous studies have demonstrated that HPV-16 oncoproteins enhanced hypoxia-inducible factor-1α (HIF-1α) protein accumulation and vascular endothelial growth factor (VEGF) and interleukin-8 (IL-8) expression in non-small cell lung cancer (NSCLC) cells, thus contributing to angiogenesis. In this study, we further investigated the role of ERK1/2 signaling pathway in HPV-16 oncoprotein-induced HIF-1α, VEGF, and IL-8 expression and in vitro angiogenesis in NSCLC cells. Our results showed that HPV-16 E6 and HPV-16 E7 oncoproteins promoted the activation of ERK1/2 signaling pathway in A549 More >