JIANRONG YANG^1,#, WAN CHEN^2,#, XING ZHOU^3,#, YAOXUAN LI^4,#, ZHIHUANG NONG⁵, LIYUAN ZHOU⁴, XUAN WEI⁴, XIAORONG PAN⁶, CHUNXIA CHEN⁷, WENSHENG LU³

BIOCELL, Vol.46, No.1, pp. 137-148, 2022, DOI:10.32604/biocell.2022.016807 - 28 September 2021

Abstract In this study, we investigated the protective effect of hyperbaric oxygen (HBO) on PC12 and H9C2 cell damage caused by oxygen-glucose deprivation/reperfusion and its possible mechanism. PC12 and H9C2 cell oxygen-glucose deprivation/reperfusion model were established. Cells were divided into a control group, model group, hyperbaric air (HBA) group and HBO group. The cell viability was detected by the CCK8 assay. Hoechst 33342 and PI staining assays and mitochondrial membrane potential (MMP) assays were used to detect cell apoptosis. The ultrastructure of cells, including autophagosomes, lysosomes, and apoptosis, were examined using a transmission electron microscope. The… More >

HUIFANG CHEN^1,2, XIAOYING ZHOU², ZONGHONG LONG², XIANGLONG TANG², HONG LI^2,*

BIOCELL, Vol.44, No.3, pp. 371-379, 2020, DOI:10.32604/biocell.2020.08893 - 22 September 2020

Abstract UQCRC1 is one of the 10 mitochondrial complex III subunits, this protein has a role in energy metabolism, myocardial protection, and neurological diseases. The upstream mechanism of the UQCRC1 protective effect on cardiomyocytes is currently unavailable. In order to explore the upstream molecules of UQCRC1 and elucidate the protective mechanism of UQCRC1 on cardiomyocytes in more detail, we focused on the nuclease-sensitive elementbinding protein 1 (YB-1). We hypothesized YB-1 acts as an upstream regulatory molecule of UQCRC1. This study found that YB-1 RNAi significantly reduces the expression of the UQCRC1 protein level (p < 0.05) and More >

XUHE GONG¹, GUANGPU FAN², HUI LIU¹, SIJIA WANG³, RONGCHONG HUANG^1,*

BIOCELL, Vol.44, No.3, pp. 329-336, 2020, DOI:10.32604/biocell.2020.09639 - 22 September 2020

Abstract This work aimed to study the beneficial role of human umbilical cord blood-derived mesenchymal stem cellconditioned medium (MSC-CM) in hypoxia-induced apoptosis in H9c2 cardiomyoblasts, in which the serine/heroine kinases (Akt) pathway would be involved. For this, CM was collected by culturing MSCs in serum-free DMEM medium for 24 h, and paracrine factors were analyzed by protein chip. H9c2 cells were divided into the following groups: control group, hypoxia group, MSC-CM intervention group (CM group), MSC-CM + Akt phosphorylation inhibitor (LY294002) group (LY group). Apoptosis of the H9c2 cells was tested with chromatin dye Hoechst 33342… More >

M. V. Kurian¹, J. M. Mullins¹, L. R. Hamilton¹, P. M. Mehl², J. K. Keevan²

Molecular & Cellular Biomechanics, Vol.3, No.4, pp. 217-218, 2006, DOI:10.32604/mcb.2006.003.217

Abstract This article has no abstract. More >