Honghui Sun^1,2,#, Ruichao Liu^1,2,#, Yueting Qi¹, Hongsheng Gao¹, Xueting Wang¹, Ning Jiang^1,2, Xiaotong Guo^1,2, Hongxia Zhang¹, Chunyan Yu^1,2,*

Phyton-International Journal of Experimental Botany, Vol.93, No.3, pp. 533-549, 2024, DOI:10.32604/phyton.2024.049417

Abstract Grapes, one of the oldest tree species globally, are rich in vitamins. However, environmental conditions such as low temperature and soil salinization significantly affect grape yield and quality. The glutamate receptor (GLR) family, comprising highly conserved ligand-gated ion channels, regulates plant growth and development in response to stress. In this study, 11 members of the VvGLR gene family in grapes were identified using whole-genome sequence analysis. Bioinformatic methods were employed to analyze the basic physical and chemical properties, phylogenetic trees, conserved domains, motifs, expression patterns, and evolutionary relationships. Phylogenetic and collinear analyses revealed that the VvGLRs were divided into three… More >

Teng Fei¹, Youmei Li¹, Bo Li², Zhaosen Xie^1,*

Phyton-International Journal of Experimental Botany, Vol.92, No.7, pp. 2117-2132, 2023, DOI:10.32604/phyton.2023.028208

Abstract The vascular system of the grapevine (Vitis vinifera L.) flower is a channel for transporting water and nutrients to the ovary. It plays an important role in the development of the ovary and fertilization through pollination. However, the vascular bundles in the flower are so tiny that they are difficult to sample and observe by traditional slicing techniques. In this study, ‘Summer Black’ grape flowers were selected as the test materials, and the tissue samples were treated by the optical clearing technique. After simple compaction, the structure and development of the vasculature were observed by common microscopy, fluorescence microscopy and… More >

Yue Wang^1,#, Xiujie Li^2,#, Yusen Wu², Zhonghui Cai¹, Bo Li², Zhaosen Xie^1,*

Phyton-International Journal of Experimental Botany, Vol.92, No.6, pp. 1859-1871, 2023, DOI:10.32604/phyton.2023.027067

Abstract It is generally agreed that many Vitis vinifera L. cultivars are self-fertile, where self-pollination often occurs before capfall in a process called cleistogamy. Therefore, it is difficult to identify the right time to remove stamens before self-pollination during the cross-breeding of grape. For this paper, we observed the process of grape flowering and measured the pollen viability and stigma receptivity of grape flowers of ‘Shine Muscat’ in order to identify the starting time of self-pollination before capfall and to provide useful information for improving the efficiency of cross-breeding. The results demonstrate that the anther is not cracked during the visible… More >

Pengfei Zhang^1,#, Yuqin Zhang^1,#, Qifeng Zhao², Tiequan Niu¹, Pengfei Wen^1,*, Jinjun Liang^1,*

Phyton-International Journal of Experimental Botany, Vol.92, No.4, pp. 1125-1138, 2023, DOI:10.32604/phyton.2023.026227

Abstract Grape pistil has an important influence on fruit size and quality. However, there were few studies on grape ovary, and the development process of the ovary is still unclear. Therefore, in this paper, four different grape varieties with different lengths of small inflorescences, namely ‘Musct Hambourg’ grape (Vitis vinifera), ‘Concord’ grape (Vitis labrusca), ‘ShanPuTao’ grape (Vitis amurensis) and ‘GongNiang2Hao’ grape (Vitis amurensis × Vitis vinifera) were used as test materials. Four varieties ovary were significant differences by means of stereomicroscope, paraffin section. The expression of ovary determining gene VvAGAMOUS (VvAG) and its development related genes VvCRABS CLAW (VvCRC) and VvAGAMOUS-LIKE 11 … More >

Kamel Jebreen^1,2,3,*, Muhammad Haroon Aftab⁴, Mohammad Issa Sowaity⁵, Zeeshan Saleem Mufti⁴, Muhammad Hussain⁶

CMC-Computers, Materials & Continua, Vol.73, No.3, pp. 4455-4463, 2022, DOI:10.32604/cmc.2022.030246

Abstract In this article, we calculate various topological invariants such as symmetric division degree index, redefined Zagreb index, VL index, first and second exponential Zagreb index, first and second multiplicative exponential Zagreb indices, symmetric division degree entropy, redefined Zagreb entropy, VL entropy, first and second exponential Zagreb entropies, multiplicative exponential Zagreb entropy. We take the chemical compound named Proanthocyanidins, which is a very useful polyphenol in human’s diet. They are very beneficial for one’s health. These chemical compounds are extracted from grape seeds. They are tremendously anti-inflammatory. A subdivision form of this compound is presented in this article. The compound named… More >

Han Lei^1,#, Junlin Li^2,#, Zhizhong Song^1,3,*

Phyton-International Journal of Experimental Botany, Vol.91, No.11, pp. 2519-2536, 2022, DOI:10.32604/phyton.2022.021268

Abstract Potassium (K⁺) is an essential macronutrient for plants to maintain normal growth and development. Shaker-like K⁺ channels and HAK/KUP/KT transporters are critical components in the K⁺ acquisition and translocation. In this study, we identified 9 Shaker-like K⁺ channel (VvK) and 18 HAK/KUP/KT transporter (VvKUP) genes in grape, which were renamed according to their distributions in the genome and relative linear orders among the distinct chromosomes. Similar structure organizations were found within each group according to the exon/intron structure and protein motif analysis. Chromosomal distribution analysis showed that 9 VvK genes and 18 VvKUP genes were unevenly distributed on 7 or… More >

HUI-YING JIN^1,2, MAO-SONG PEI^1,2, DA-LONG GUO^1,2,*

BIOCELL, Vol.46, No.4, pp. 941-949, 2022, DOI:10.32604/biocell.2022.016718

Abstract To understand the molecular characteristics of the miR477 gene family of grape (Vvi-miR477) and to predict its target genes, the Vvi-miR477 genes were identified from previous small RNA sequencing data, then phylogenetic analysis and prediction of target gene were conducted. The Vvi-miR477 family consists of two precursor sequences and three mature sequences. The miR477 family members were mostly 19-22nt in length. The sequence is relatively conservative. Vvi-MIR477a and Vvi-MIR477b are located on chromosomes 1 and 2, respectively. These precursor sequences can form the typical stable stem-loop structure. Their minimum folding free energy is −39.10 kcal/mol and −50.90 kcal/mol, respectively. The… More >

Wei Tan¹, Mingxiu Xu¹, Siqi Xie¹, Yan Zhang¹, Shuai Wu¹, Qinyan Zou¹, Qifeng Zhao², Qingliang Li^3,*

Phyton-International Journal of Experimental Botany, Vol.90, No.2, pp. 553-570, 2021, DOI:10.32604/phyton.2021.014082

Abstract To understand the anthocyanin characteristics of wine grape varieties, the anthocyanin composition and content of 31 wine grape varieties were analyzed to explore the use of anthocyanins as chemical fingerprints to distinguish varieties. Results showed that a total of 21 anthocyanins were detected in the skins, including cyanidin, delphinidin, petunidin, peonidin and malvidin 3-monoglucosides (or 3,5-diglucosides) along with the corresponding acetyl and p-coumaroyl derivatives. The highest and lowest total amount of anthocyanins were detected in ‘Ruby Cabernet’ and ‘Muscat Rouge’, respectively. In the 21 Vitis vinifera grapes, there were 3~11 monoglucoside anthocyanins detected, however, there were 4 to 9 monoglucoside… More >

Murad Awad¹, Photini V. Mylona², Alexios N. Polidoros^1,*

Phyton-International Journal of Experimental Botany, Vol.89, No.4, pp. 905-923, 2020, DOI:10.32604/phyton.2020.011235

Abstract Discrimination among grapevine varieties based on quantitative traits, such as flowering, veraison and ripening dates is crucial for variety selection in the context of climate change and in breeding programs. These traits are under complex genetic control for which 6 linked SSR loci (VVS2, VVIn16, VMC7G3, VrZAG29, VMC5G7, and VVIB23) have been identified. Using these markers in HRM-PCR analysis, we assessed genetic diversity among a large collection of 192 grapevine varieties. The grapevine germplasm used encompasses the majority of Greek vineyard with 181 varieties, 3 prominent foreign varieties and 11 varieties of Palestinian origin. The SSR markers used were highly… More >

Jiménez-Martínez JH¹, MG Gutiérrez-Martínez², O Franco-Mora¹, A González-Huerta², AT Gutiérrez-Ibáñez²

Phyton-International Journal of Experimental Botany, Vol.82, pp. 107-112, 2013, DOI:10.32604/phyton.2013.82.107

Abstract Two accessions of wild grapevines (Vitis spp.) native to Puebla State, Nealticán and Teziutlán, and other two native to Temascaltepec (1 and 2), Mexico State, were cultured in vitro using both Murashige-Skoog (MS) and woody plant medium (WPM) mediums. There were no differences between mediums for shoot generation. However, differences were shown among accessions for explant vigour, callus development, number of roots, leaves and nodes per explant. Rooting was evaluated only in MS medium by adding naphthalene acetic acid (NAA), indole butyric acid (IBA) or indole acetic acid (IAA) at 0.5 mg/L. With the exception of Temascaltepec 2, NAA generated… More >