XUHUI ZHAO^1,2, XIAOMIN HUANG¹, CHUNYAN DANG², XIA WANG¹, YUJIAO QI³, HONGLING LI^2,*

Oncology Research, Vol.32, No.5, pp. 999-1009, 2024, DOI:10.32604/or.2024.046679

Abstract Background: EBV-miR-BARTs exhibit significant relevance in epithelial tumors, particularly in EBV-associated gastric and nasopharyngeal cancers. However, their specific mechanisms in the initiation and progression of gastric cancer remain insufficiently explored. Material and Methods: Initially, EBV-miRNA-BART6-5p and its target gene SMAD4 expression were assessed in EBV-associated gastric cancer tissues and cell lines. Subsequent transfection induced overexpression of EBV-miRNA-BART6-5p in AGS and MKN-45, and downregulation in EBV-positive cells (SUN-719). The subsequent evaluation aimed to observe their impact on gastric cancer cell proliferation, migration, and glycolytic processes, with the TGF-β/SMAD4 signaling pathway value clarified using a TGF-β inhibitor. Results: EBV-miRNA-BART6-5p exhibits pronounced upregulation… More >

CHENYANG CHEN^1,*, JUAN WEN¹, WEI HUANG¹, JIANG LI^2,*

BIOCELL, Vol.47, No.11, pp. 2423-2433, 2023, DOI:10.32604/biocell.2023.044459

Abstract Background: Pulmonary arterial hypertension (PAH) is a chronic and progressive disease that is strongly associated with dysregulation of glucose metabolism. Alterations in nuclear receptor subfamily 4 group A member 1 (NR4A1) activity alter the outcome of PAH. This study aimed to investigate the effects of NR4A1 on glycolysis in PAH and its underlying mechanisms. Methods: This study included twenty healthy volunteers and twenty-three PAH patients, and plasma samples were collected from the participants. To mimic the conditions of PAH in vitro, a hypoxia-induced model of pulmonary artery smooth muscle cell (PASMC) model was established. The proliferation of PASMCs was assessed… More > Graphic Abstract

LIJUN DU¹, KAIKAI DOU¹, NIANHAI LIANG^1,2, JIANMIN SUN¹, RU BAI^1,*

BIOCELL, Vol.47, No.3, pp. 547-554, 2023, DOI:10.32604/biocell.2023.025048

Abstract Background: The Warburg effect is considered as a hallmark of various types of cancers, while the regulatory mechanism is poorly understood. Our previous study demonstrated that miR-194-5p directly targets and regulates insulin-like growth factor1 receptor (IGF1R). In this study, we aimed to investigate the role of miR-194-5p in the regulation of the Warburg effect in ovarian cancer cells. Methods: The stable ovarian cell lines with miR-194-5p overexpression or silencing IGF1R expression were established by lentivirus infection. ATP generation, glucose uptake, lactate production and extracellular acidification rate (ECAR) assay were used to analyze the effects of aerobic glycolysis in ovarian cancer… More >

ZECHAO LU^1,#, FUCAI TANG^1,#, HAOBIN ZHOU^2,#, ZEGUANG LU^3,#, WANYAN CAI^4,#, JIAHAO ZHANG⁵, ZHICHENG TANG⁶, YONGCHANG LAI^1,*, ZHAOHUI HE^1,*

BIOCELL, Vol.47, No.2, pp. 339-350, 2023, DOI:10.32604/biocell.2023.023750

Abstract Background: Establishing an appropriate prognostic model for PCa is essential for its effective treatment. Glycolysis is a vital energy-harvesting mechanism for tumors. Developing a prognostic model for PCa based on glycolysis-related genes is novel and has great potential. Methods: First, gene expression and clinical data of PCa patients were downloaded from The Cancer Genome Atlas (TCGA) and Gene Expression Omnibus (GEO), and glycolysis-related genes were obtained from the Molecular Signatures Database (MSigDB). Gene enrichment analysis was performed to verify that glycolysis functions were enriched in the genes we obtained, which were used in non-negative matrix factorization (NMF) to identify clusters.… More >

Rong Zeng^1,#, Lin Liu^1,2,#, Jingshan Zhao^1,2,3,#, Wenmei Zhang³, Guohong Zhang¹, Yunfeng Li^1,2,*

Oncologie, Vol.24, No.3, pp. 483-498, 2022, DOI:10.32604/oncologie.2022.025148

Abstract Background: Breast cancer (BC) is the most frequent type of gynecology tumors with high morbidity and mortality. Ampelopsin, the main active compound of Ampelopsis grossedentata, exerts an anti-tumor effect on a variety of cancers. However, the anti-cancer role of ampelopsin in BC remains unclear. The aim of this study is to explore the mechanism of ampelopsin against breast cancer. Materials and Methods: The target genes of ampelopsin in the treatment of breast cancer were determined and analyzed by network pharmacology and molecular docking. Cytoscape software was used to identify the core target genes and construct a protein–protein interaction (PPI) network.… More >

FEI LIU¹, JIAZHANG WEI², JIAO LAN¹, YONGLI WANG², JIANXIANG YE³, CHENG Su¹, MINGZHENG MO¹, FENGZHU TANG², BING LI², MIN LI², WEIMING DENG², LINSONG YE², Wenlin HUANG², JINGJIN WENG², WEI JIAO^1,*, SHENHONG QU^2,*

BIOCELL, Vol.46, No.12, pp. 2659-2669, 2022, DOI:10.32604/biocell.2022.022886

Abstract Our previous studies suggested a potential interaction between the POK erythroid myeloid ontogenic factor ZBTB7A and glucose transporter 1 (GLUT1) in nasopharyngeal carcinoma (NPC). This study was designed to confirm the interaction and further evaluate the precise mechanism by which ZBTB7A and GLUT1 regulate NPC development. The binding sites between ZBTB7A and the promoter of GLUT1 were predicted by bioinformatics. Gene expression was measured by quantitative real-time polymerase chain reaction (qPCR), western blotting, and immunohistochemistry. The activities of key glycolysis enzymes, including hexokinase (HK), pyruvate kinase (PK), lactate dehydrogenase (LDH), and lactate, were detected using specific enzyme-linked immunosorbent assay kits.… More >

HUAMIN WANG^1,#, YANTING WU^1,#, ZHENLEI WANG^2,#, YUHANG CHEN¹, JINYU MO¹, WEN GUAN¹, YALI ZHANG¹, HONGLIANG YAO^1,*

Oncology Research, Vol.29, No.3, pp. 201-215, 2021, DOI:10.32604/or.2022.03553

Abstract LncRNAs and metabolism represents two factors involved in cancer initiation and progression. However, the interaction between lncRNAs and metabolism remains to be fully explored. In this study, lncRNA FEZF1-AS1 (FEZF1- AS1) was found upregulated in colon cancer after screening all the lncRNAs of colon cancer tissues deposited in TCGA, the result of which was further confirmed by RNAscope staining on a colon tissue chip. The results obtained using FEZF1- AS1 knockout colon cancer cells (SW480 KO and HCT-116 KO) constructed using CRISPR/Cas9 system confirmed the proliferation, invasion, and migration-promoting function of FEZF1-AS1 in vitro. Mechanistically, FEZF1-AS1 associated with the mitochondrial… More >

Ting Yu^*1, Lei Li^†1, Wenyan Liu^*, Bailiu Ya^*, Hongju Cheng^*, Qing Xin^*

Oncology Research, Vol.27, No.5, pp. 525-532, 2019, DOI:10.3727/096504018X15179668157803

Abstract Hypoxia-induced chemoresistance is a major obstacle in the development of effective cancer therapy. In our study, the reversal abilities of NADPH oxidase 4 (NOX4) silence on hypoxia resistance and the potential mechanism were investigated. Our data showed that the expression of NOX4 was upregulated in human neuroblastoma cells SH-SY5Y under hypoxia condition time dependently. Knockdown of NOX4 expression by siRNA inhibited glycolysis induced by hypoxia through decreasing the expression of glycolysis-related proteins (HIF-1 , LDHA, and PDK1), decreasing glucose uptake, lactate production, and ROS production, while increasing mitochondria membrane potential. Moreover, NOX4 silence inhibited cell growth under hypoxia condition through… More >

Xiaohua Gu, Hongxiang Luo^*, Shiwei Lv, Peng Chen

Journal of Renewable Materials, Vol.9, No.7, pp. 1253-1266, 2021, DOI:10.32604/jrm.2021.014876

Abstract Dramatically increasing waste polyurethane rigid foam (WPRF) draws the attention of the world. A mixture of ethylene glycol (EG) and diethylene glycol (DEG) is used as glycolysis agents. WPRF was subjected to alcoholysis using different catalysts which are titanium ethylene glycol and potassium hydroxide to obtain recycled polyol, respectively. The effect of a different catalyst on the viscosity and hydroxyl value of recycled polyol is discussed. The regenerated polyurethane (RPU) is performed using the recycled polyol. Infrared spectrum, compressive strength, apparent density, water absorption, scanning electron microscope, and thermogravimetric analysis are carried out to investigate the effect of WPRF degradation… More >

SUFANG ZHANG^1,2,#, XIANG LV^1,#, LI LI^3,#, YINGBIN LUO¹, HUINAN XIANG¹, LIXIN WANG^2,*, YAN LI^1,*

BIOCELL, Vol.45, No.1, pp. 167-175, 2021, DOI:10.32604/biocell.2021.013636

Abstract Chemotherapy is widely used for non-small cell lung cancer (NSCLC) patients at a late stage; however, NSCLC patients often acquire resistance to chemotherapeutic drugs, thus limiting the therapy efficacy. Melittin, a major component of bee venom, possesses anti-tumor activity in various cancer cells. Here, we examined the effects of melittin on A549/DDP cisplatin-resistant lung adenocarcinoma cells and xenografts formed from this cell line and investigated the possible target of melittin. Treatment with melittin resulted in the induction of cell apoptosis, glycolysis inhibition, and reduction of phosphorylated AKT (p-AKT) in A549/DDP cells. We also identified that tripartite motif-containing 8 (TRIM8) was… More >