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  • Open Access

    ARTICLE

    Involvement of the ABA- and H2O2-Mediated Ascorbate–Glutathione Cycle in the Drought Stress Responses of Wheat Roots

    Mengyuan Li1, Zhongye Gao1,2, Lina Jiang1, Leishan Chen1,2,*, Jianhui Ma1,*

    Phyton-International Journal of Experimental Botany, Vol.93, No.2, pp. 329-342, 2024, DOI:10.32604/phyton.2024.046976 - 27 February 2024

    Abstract Abscisic acid (ABA), hydrogen peroxide (H2O2) and ascorbate (AsA)–glutathione (GSH) cycle are widely known for their participation in various stresses. However, the relationship between ABA and H2O2 levels and the AsA–GSH cycle under drought stress in wheat has not been studied. In this study, a hydroponic experiment was conducted in wheat seedlings subjected to 15% polyethylene glycol (PEG) 6000–induced dehydration. Drought stress caused the rapid accumulation of endogenous ABA and H2O2 and significantly decreased the number of root tips compared with the control. The application of ABA significantly increased the number of root tips, whereas the application… More >

  • Open Access

    ARTICLE

    Evaluation of combined detection of nuclear factor erythroid 2-related factor 2 and glutathione peroxidase 4 in primary hepatic carcinoma and preliminary exploration of pathogenesis

    JIE DUAN, AIDONG GU*, WEI CHEN, CHANGHAO CHEN, FANGNAN SONG, FAXI CHEN, FANGFANG JIANG, HUIWEN XING

    BIOCELL, Vol.47, No.12, pp. 2609-2615, 2023, DOI:10.32604/biocell.2023.042472 - 27 December 2023

    Abstract Objective: This study aims to analyze the clinical significance and mechanism of nuclear factor erythroid 2-related factor 2 (NRF2) and glutathione peroxidase 4 (GPX4) in primary hepatic carcinoma (PHC). Methods: The expression of NRF2 and GPX4 in peripheral blood of patients with PHC was determined to analyze the diagnostic value of the two combined for PHC. The prognostic significance of NRF2 and GPX4 was evaluated by 3-year follow-up. Human liver epithelial cells THLE-2 and human hepatocellular carcinoma cells HepG2 were purchased, and the expression of NRF2 and GPX4 in the cells was determined. NRF2 and GPX4… More > Graphic Abstract

    Evaluation of combined detection of nuclear factor erythroid 2-related factor 2 and glutathione peroxidase 4 in primary hepatic carcinoma and preliminary exploration of pathogenesis

  • Open Access

    REVIEW

    Fe-dependent cellular alterations of oxidative balance in aquatic organisms. Could be ferroptosis involved?

    PAULA MARIELA GONZÁLEZ1,2, JOAQUIN CABRERA1,2, SUSANA PUNTARULO1,2,*

    BIOCELL, Vol.47, No.5, pp. 1177-1189, 2023, DOI:10.32604/biocell.2023.027107 - 10 April 2023

    Abstract

    The purpose of this review is to briefly summarize the central role of iron (Fe) in terms of cellular alterations of the oxidative/protective balance with special emphasis on its possible involvement in ferroptosis-dependent disruption in aquatic organisms. In ferroptotic cells or tissues, the intracellular Fe level increases; meanwhile the treatment with Fe chelators limits ferroptosis. Eukaryotic algae can assimilate Fe from the environment through several mechanisms, and aquatic animals incorporate dissolved Fe and Fe bound to both inorganic particles and organic matter. The central role of lipid peroxidation mediating ferroptosis was demonstrated in some algae

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  • Open Access

    ARTICLE

    β-Cyclodextrin-Based Nitrosoglutathione Improves the Storage Quality of Peach by Regulating the Metabolism of Endogenous Nitric Oxide, Hydrogen Sulfide, and Phenylpropane

    Chen Chen, Shuhua Zhu*

    Phyton-International Journal of Experimental Botany, Vol.92, No.4, pp. 1091-1107, 2023, DOI:10.32604/phyton.2023.026331 - 06 January 2023

    Abstract Nitrosoglutathione (GSNO) and β-cyclodextrin (β-CD) exhibit positive roles in regulating fruit quality. However, there are few reports about the effects of GSNO and β-CD on enhancing storability and boosting nitric oxide (NO), hydrogen sulfide (H2S), and phenylpropane metabolism in fruits during storage. “Xintaihong” peach were treated with 0.5, 1.0, 1.5 mmol L−1 GSNO in 0.5% (w/v) β-CD solution (GSNO/β-CD). The effects of GSNO/β-CD on endogenous NO, H2S, and phenylpropane metabolism were investigated. Treatment with GSNO/β-CD increased the color difference of peach and inhibited the increase of respiratory intensity, weight loss, and relative conductivity. Treatment with 1.0 mmol… More >

  • Open Access

    ARTICLE

    Exogenous Selenium Mitigates Salt Stress in Soybean by Improving Growth, Physiology, Glutathione Homeostasis and Antioxidant Defense

    Hesham F. Alharby1, Mirza Hasanuzzaman2,*, Hassan S. Al-Zahrani1, Khalid Rehman Hakeem1

    Phyton-International Journal of Experimental Botany, Vol.90, No.2, pp. 373-388, 2021, DOI:10.32604/phyton.2021.013657 - 07 February 2021

    Abstract The mechanism of selenium (Se)-induced salt tolerance was studied in moderately sensitive soybean (Glycine max L.) plants. To execute this view, soybean plants were imposed with salt stress (EC 6 dS m−1 ) applying NaCl. In other treatments, Se (0, 25, 50 and 75 µM Na2SeO4) was sprayed as co-application with that level of salt stress. Plant height, stem diameter, leaf area, SPAD value decreased noticeably under salt stress. Altered proline (Pro) level, together with decreased leaf relative water content (RWC) was observed in salt-affected plants. Salt stress resulted in brutal oxidative damage and increased the content… More >

  • Open Access

    ARTICLE

    Phytohormonal and metabolism analysis of Brassica rapa L. ssp. pekinensis with different resistance during Plasmodiophora brassicae infection

    MEI LAN#, JINGFENG HU#, HONGLI YANG, LIQIN ZHANG, XUEZHONG XU, JIANGMING HE*

    BIOCELL, Vol.44, No.4, pp. 751-767, 2020, DOI:10.32604/biocell.2020.012954 - 24 December 2020

    Abstract Clubroot of Chinese cabbage (Brassica rapa L. ssp. pekinensis), caused by the obligate parasite Plasmodiophora brassicae, accounts for serious yield losses. The aim of our study was to explore the phytohormone levels and metabolome changes in the roots of resistant and susceptible B. rapa genotypes at a late stage of infection, i.e., 28 days post-infection. Both genotypes showed decreased auxin levels after P. brassicae infection except for indole-3-acetic acid. Overall, the susceptible genotype had higher auxin and cytokinin levels after infection, with the exception of trans-zeatin and 3- indolebutyric acid as compared to the resistant genotype. Jasmonic acid levels declined… More >

  • Open Access

    ARTICLE

    Trichoderma-Induced Improvement in Growth, Photosynthetic Pigments, Proline, and Glutathione Levels in Cucurbita pepo Seedlings under Salt Stress

    Mona H. Soliman1, Taghreed S. Alnusaire2, Nessreen F. Abdelbaky3,4, Aisha A. M. Alayafi5, Mirza Hasanuzzaman6,*, Mohamed M. Rowezak2, Mohamed El-Esawi7, Amr Elkelish8

    Phyton-International Journal of Experimental Botany, Vol.89, No.3, pp. 473-486, 2020, DOI:10.32604/phyton.2020.08795 - 22 June 2020

    Abstract Salt stress is one of the major abiotic stress in plants. However, traditional approaches are not always efficient in conferring salt tolerance. Experiments were conducted to understand the role of Trichoderma spp. (T. harzianum and T. viride) in growth, chlorophyll (Chl) synthesis, and proline accumulation of C. pepo exposed to salinity stress. There were three salt stress (50, 100, and 150 mM NaCl) lavels and three different Trichoderma inoculation viz. T. harzianum, T. viride, and T. harzianum + T. viride. Salt stress significantly declined the growth in terms of the shoot and root lengths; however, it was improved by the inoculation of Trichoderma spp.… More >

  • Open Access

    ARTICLE

    Crude Flavonoid Extract of Medicinal Herb Zingibar officinale Inhibits Proliferation and Induces Apoptosis in Hepatocellular Carcinoma Cells

    Ayman I. Elkady*†, Osama A. Abu-Zinadah*, Rania Abd El Hamid Hussein‡§

    Oncology Research, Vol.25, No.6, pp. 897-912, 2017, DOI:10.3727/096504016X14816352324532

    Abstract There is an urgent need to improve the clinical management of hepatocellular carcinoma (HCC), one of the most common causes of global cancer-related deaths. Zingibar officinale is a medicinal herb used throughout history for both culinary and medicinal purposes. It has antioxidant, anticarcinogenic, and free radical scavenging properties. Previously, we proved that the crude flavonoid extract of Z. officinale (CFEZO) inhibited growth and induced apoptosis in several cancer cell lines. However, the effect of the CFEZO on an HCC cell line has not yet been evaluated. In this study, we explored the anticancer activity of CFEZO against… More >

  • Open Access

    ARTICLE

    Selenium invoked antioxidant defense system in Azolla caroliniana plant

    Hassan AMA, EM Mostafa

    Phyton-International Journal of Experimental Botany, Vol.85, pp. 262-269, 2016, DOI:10.32604/phyton.2016.85.262

    Abstract Plants of Azolla caroliniana were treated with different selenium concentrations (0, 1, 2, 5, 7, 10 ppm) for seven days. Selenium (Se) content in Azolla plants increased significantly with increasing Se concentrations in the culture media up to 5 ppm. This indicated that Azolla plants were a good accumulator for Se. Selenium accumulation determined changes in Azolla biomass, doubling time and relative growth rates. Treatment of Azolla plants with low concentrations of Se (1 ppm) resulted in a significant increase in biomass. This was accompanied by a reduction in hydrogen peroxide and malondialdehyde (MDA) contents; the decrease percentages… More >

  • Open Access

    ARTICLE

    Superoxide and hydrogen peroxide productions by NO-inhibited complex III

    Darío E. IGLESIAS*, Silvina S. BOMBICINO, Alberto BOVERIS, Laura B. VALDEZ

    BIOCELL, Vol.40, No.1, pp. 27-30, 2016, DOI:10.32604/biocell.2016.40.027

    Abstract Complex III plays a central role in the mitochondrial respiratory chain transferring electrons from ubiquinol to cytochrome c and pumping protons to the intermembrane space, contributing to the protonmotive force. Furthermore, complex III can act as a source of O2 •- in the presence of ubiquinol and antimycin, an expermiental condition in which the oxidation of the cytochrome b hemes is blocked. The O2 •- dismutation catalyzed by superoxide dismutase produces H2O2, a known second messenger in redox signalling. Results from our laboratory have shown that NO, released from GSNO or from SPER -NO or generated… More >

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