Huifang Lv, Honglin Hou, Huijun Lei, Caiyun Nie, Beibei Chen, Liangyu Bie, Lili Han, Xiaobing Chen

Oncology Research, Vol.28, No.3, pp. 225-236, 2020, DOI:10.3727/096504019X15753718797664

Abstract S100 binding protein A16 (S100A16) expression levels are closely associated with microRNA (miRNA) processing. Higher levels of S100A16 are reported during the progression of many cancers. Our study mainly explored the interaction between S100A16 and miR-6884-5p in gastric cancer (GC). Quantitative real-time polymerase chain reaction (qRT-PCR) was used to determine the level of S100A16 and miR-6884-5p in GC tissues and cell lines. The si-S100A16, pcDNA-S100A16, miR-6884-5p mimic or inhibitor was transfected into GC cells, and the effects of S100A16 and miR-6884-5p on the proliferation, invasion, and epithelial–mesenchymal transition (EMT) were explored by qRT-PCR and Western… More >

Lihua Jiang^*1, Wenchuan Yang^*1, Weishi Bian^†, Hailin Yang^*, Xia Wu^*, Yuhua Li^*, Wen Feng^*, Xuejian Liu^*

Oncology Research, Vol.27, No.1, pp. 19-27, 2019, DOI:10.3727/096504018X15193469240508

Abstract The dysregulation of microRNAs (miRNAs) plays an important function in the onset and progression of gastric cancer (GC). In addition, aberrantly expressed miRNAs affect the chemosensitivity of GC cells to chemotherapeutic drugs. Hence, miRNA-based targeted therapy might be applied to treat patients with GC exhibiting chemotherapeutic resistance. In this study, miRNA-623 (miR-623) expression was downregulated in GC tissues and cell lines. Functional analysis showed that the restored miR-623 expression could inhibit the proliferation of GC cells and enhance their chemosensitivity to 5-FU via the cell apoptosis pathway. Cyclin D1 (CCND1) was identified as a direct More >

Jianye Li^*, Bin Zhang^†, Jizhao Cui^‡, Zhen Liang^§, Kexia Liu^*

Oncology Research, Vol.27, No.7, pp. 789-799, 2019, DOI:10.3727/096504018X15444387696532

Abstract Many studies have shown that downregulated miR-203 level is in a variety of cancers including gastric cancer (GC). However, the precise molecule mechanisms of miR-203 in GC have not been well clarified. In the current study, we investigated the biological functions and molecular mechanisms of miR-203 in GC cell lines. We found that miR-203 is downregulated in GC tissues and cell lines. Moreover, the low level of miR-203 was associated with increased expression of annexin A4 in GC tissues and cell lines. The invasion and EMT of GC cells were suppressed by overexpression of miR-203.… More >

Chao Liu^*†, Min Jian^‡, Hong Qi^†, Wei-Zheng Mao^†

Oncology Research, Vol.27, No.3, pp. 389-397, 2019, DOI:10.3727/096504018X15223159811838

Abstract Recently, microRNAs (miRNAs) have been reported to participate in multiple biological processes. However, the effects of miR-495 on gastric cancer (GC) remain unclear. The purpose of this study was to explore the functions of miR-495 in GC cell proliferation, metastasis, and apoptosis. SGC-7901 and BGC-823 cell lines were transfected with miR-495 mimic, miR-495 inhibitor, and negative controls (mimic control and inhibitor control). The expressions of miR-495, cell viability, migration, apoptosis, and apoptosis-related factors were examined by qRT-PCR, trypan blue staining, Transwell, flow cytometry, and Western blot, respectively. Simultaneously, key factor expression levels of EMT were… More >

Qiaorong Li^*, Geng Wang^†, Hong Wang^‡

Oncology Research, Vol.26, No.9, pp. 1345-1353, 2018, DOI:10.3727/096504018X15180508535835

Abstract The expression of miR-126 and serine–arginine protein kinase 1 (SRPK1) are linked to tumor development; nevertheless, its role in the tumor growth and invasion of gastric cancer (GC) and the underlying mechanism have not been clarified. Here the expression and role of miR-126 and SRPK1 were investigated in GC tissues and cells by in vitro assay, and then targets of miR-126 were identified by dual-luciferase reporter assay. In this study, miR-126 expression was downregulated and associated with lymph node metastasis and poor prognosis as well as SRPK1 expression. In vitro assay revealed that miR-126 obviously More >

Mao Guoping^*, Liu Ran^†, Qin Yanru^*

Oncology Research, Vol.26, No.7, pp. 1023-1029, 2018, DOI:10.3727/096504018X15151515028670

Abstract Recent studies have suggested that the dysregulation of microRNAs (miRNAs) plays a critical role in the progression of human cancers, including gastric cancer (GC). miR-143 had been reported to function as a tumor suppressor in GC. However, the exact molecular mechanism of how miR-143 participates in GC progression remains to be determined. In this present study, we revealed that the expression of miR-143 was significantly downregulated in human GC tissues and cell lines compared with normal tissues and a normal gastric epithelium cell line. In addition, upregulation of the expression of miR-143 in a GC More >

Yinfang Wu^*, Jun Zhang^†, Yu Zheng^‡, Cheng Ma^‡, Xing-E Liu^§, Xiaodong Sun^*‡

Oncology Research, Vol.26, No.1, pp. 157-171, 2018, DOI:10.3727/096504017X15031557924150

Abstract This work aims to elucidate the effects and the potential underlying mechanisms of microRNA-216a-3p (miR- 216a-3p) on the proliferation, migration, and invasion of gastric cancer (GC) cells. In this study, we revealed that the expression of miR-216a-3p was significantly elevated in GC tissues and cell lines. The different expression level of miR-216a-3p was firmly correlated with clinicopathological characteristics of GC patients. We next demonstrated that upregulation of miR-216a-3p could dramatically promote the ability of proliferation, migration, and invasion of GC cells using a series of experiments, whereas downregulation essentially inhibited these properties. Additionally, through bioinformatics More >

Ce Ji^*, Ying Zhao^*, You-Wei Kou^*, Hua Shao^*, Lin Guo^*, Chen-Hui Bao^*, Ben-Chun Jiang^*, Xin-Ying Chen^*, Jing-Wei Dai^†, Yu-Xin Tong^‡, Ren Yang^*, Wei Sun^*, Qiang Wang^*

Oncology Research, Vol.26, No.1, pp. 83-93, 2018, DOI:10.3727/096504017X14928634401204

Abstract Gastric cancer (GC) is one of the most common cancers in the world. The cathepsin F (CTSF) gene has recently been found to participate in the progression of several types of cancer. However, the clinical characteristics and function of CTSF in GC as well as its molecular mechanisms are not clear. Six GC cell lines and 44 paired adjacent noncancerous and GC tissue samples were used to assess CTSF expression by quantitative polymerase chain reaction (qPCR). We used lentivirus-mediated small hairpin RNA (Lenti-shRNA) against CTSF to knock down the expression of CTSF in GC cells.… More >

Yingchao Li^*, Xiaoni Yan^*, Li Ren^*, Yang Li^†

Oncology Research, Vol.26, No.1, pp. 1-8, 2018, DOI:10.3727/096504016X14772410356982

Abstract Epithelial–mesenchymal transition (EMT) is one of the most important mechanisms in the metastasis of various cancers, including gastric cancer (GC). In this study, we explored the putative significance of miR-644a and its role in EMT-mediated metastasis of GC. We first detected the expression of miR-644a in a cohort of 107 GC tissues using quantitative RT-PCR. The expression of miR-644a was suppressed in GC tissues and was associated with a later clinical stage and tumor metastasis. Restoring the expression of miR-644a could significantly suppress the migration and invasion of HGC-27 and SGC-7901 cells, which might be More >

Sheqing Ji^*, Youxiang Zhang^†, Binhai Yang^‡

Oncology Research, Vol.25, No.9, pp. 1633-1641, 2017, DOI:10.3727/096504017X14878528144150

Abstract Increased expression of YEATS domain containing 4 (YEATS4) has been reported to have a correlation with progression in many types of cancer. However, the mechanism by which it promotes the development of gastric cancer (GC) is rarely reported. This study aimed to investigate the effect of YEATS4 on cell proliferation and tumor progression. The mRNA and protein expressions of YEATS4 in GC tissues and cell lines were analyzed. BGC-823 cells then overexpressed or silenced YEATS4 by transfection of different plasmids. The regulatory effect of YEATS on cell viability, colony formation, cell apoptosis, and tumor growth… More >