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Search Results (7)
  • Open Access

    ARTICLE

    Integrated GC-MS and LC-MS-Based Untargeted Metabolomics Reveals Diverse Metabolites in Fermented Pine Needles

    Yinyu Xu1, Zheng Li1, Qian Wen2, Hao He1, Pengtu Shi1, Wenhua Zhou2,*

    Phyton-International Journal of Experimental Botany, Vol.93, No.9, pp. 2367-2382, 2024, DOI:10.32604/phyton.2024.047949 - 30 September 2024

    Abstract The diversity of metabolites produced in fermented pine needles at different stages of fermentation has rarely been investigated. In the present study, untargeted metabolomic analysis using GC-MS and LC-MS was performed to detect metabolites in fermented pine needles at different fermentation stages. A total of 30 samples of pine needles fermented first with yeast (Saccharomyces cerevisiae) and then with a mixed bacterial culture of Lactobacillus fermentum CECT5716 and Bifidobacterium Breve M16V, were used to detect differential metabolites at different stages of fermentation. Pearson’s correlation analysis was used to determine correlations between metabolites and key microbial communities. A… More >

  • Open Access

    ARTICLE

    Identification of Secondary Metabolites in Tunisian Tilia platyphyllos Scop. Using MALDI-TOF and GC-MS

    Ayda Khadhri1, Mohamed Mendili1, Marwa Bannour-Scharinger1, Eric Masson2, Antonio Pizzi2,*

    Journal of Renewable Materials, Vol.12, No.4, pp. 827-842, 2024, DOI:10.32604/jrm.2024.046950 - 12 June 2024

    Abstract This study is the first to evaluate the phytochemical content and biological properties of Tunisian T. platyphyllos Scop. A total of 23 compounds of essential oils were identified by gas chromatography-mass spectrometry (GC-MS) analysis of bracts and fruit extracts. The results show that oxygenated monoterpenes were the dominant class of essential oils. The phenolic composition was investigated by matrix-assisted laser desorption/ionization-time of flight (MALDI-TOF). The analysis showed that the chemical profiles of the ethanolic extracts of bracts and fruits are substantially similar. The highest polyphenol content was found in the ethanolic extracts of the fruits (7.65… More > Graphic Abstract

    Identification of Secondary Metabolites in Tunisian <i>Tilia platyphyllos</i> Scop. Using MALDI-TOF and GC-MS

  • Open Access

    ARTICLE

    Non-Polar Chemical Constituents of Atemoya and Evaluation of the Cytotoxic and Antimicrobial Activity

    Suzana Vieira Rabêlo1, Fernanda Granja da Silva Oliveira2, Michele Miriam Calixto de Lira3, Livia Macedo Dutra3, Adilson Sartoratto4, Marta Cristina Teixeira Duarte4, Maria Claudia dos Santos Luciano5, Maria Francilene Souza Silva5, Cláudia do Ó Pessoa5, Manoel Odorico de Moraes Filho5, Emmanoel Vilaça Costa6, Jackson Roberto Guedes da Silva Almeida1,3,*

    Phyton-International Journal of Experimental Botany, Vol.90, No.3, pp. 921-931, 2021, DOI:10.32604/phyton.2021.013305 - 30 March 2021

    Abstract This study aimed to identify the chemical composition of essential oil from fruits (EOAF) and the hexanic crude extract from aerial parts (At-Hex) of atemoya (Annona cherimola x Annona squamosa), a hybrid belonging to the Annonaceae family. Cytotoxic and antimicrobial activity was also evaluated. OEAF was obtained by hydrodistillation using a Clevenger apparatus, and their composition was analyzed by gas chromatography-mass spectrometry (GC-MS) analyses. Cytotoxicity was tested against human tumor cell lines HCT-116 (colon carcinoma), SF-295 (glioblastoma), OVCAR-8 (ovarian carcinoma) and HL60 (leukemia) using 3-(4,5-dimethyl-2-thiazolyl)-2,5- diphenyl-2H-tetrazolium bromide (MTT) assay, while antimicrobial activity was conducted by bioauthography method More >

  • Open Access

    ARTICLE

    GC-MS Analysis of Metabolites in Filling Grains of Rice-Tartary Buckwheat (Fagopyrum tataricum) in Comparison to Conventional Tartary Buckwheat

    Chenggang Liang1,#, Chunyu Wei1,#, Yan Wang1,*, Wujuan Yu1, Kai Liao1, Taoxiong Shi1, Dabing Xiang2,3, Qijiao Chen1

    Phyton-International Journal of Experimental Botany, Vol.90, No.3, pp. 871-893, 2021, DOI:10.32604/phyton.2021.014242 - 30 March 2021

    Abstract Rice-Tartary buckwheat (RTB) is a special germplasm of Tartary buckwheat. In this study, the appraisal of taste quality between RTB and conventional Tartary buckwheat (CTB) was presented, and the metabolites in kernels at three typical grain filling stage (GFS) were investigated. Unlike CTB, RTB showed thin shell seeds without longitudinal furrows at maturity, which was easily artificially dehulled. Sense organ test indicated that RTB exhibited better taste quality because of the higher values of appearance, viscosity, taste and summary were appraised. In total, 92 metabolites were identified in kernels using GM-MS metabolomics platform. The levels… More >

  • Open Access

    ARTICLE

    Chemical Constituents of Pedicularis longiflora var. tubiformis (Orobanchaceae), a Common Hemiparasitic Medicinal Herb from the Qinghai Lake Basin, China

    Feng Liu1,2,3, Zilan Ma1,2,3, Marcos A. Caraballo-Ortiz4, Hui Zhang5, Xu Su1,2,3, Yuping Liu1,2,3,*

    Phyton-International Journal of Experimental Botany, Vol.89, No.4, pp. 1083-1090, 2020, DOI:10.32604/phyton.2020.011239 - 09 November 2020

    Abstract Pedicularis longiflora var. tubiformis (Orobanchaceae) is an abundant parasitic herb mainly found in the Xiaopohu wetland of the Qinghai Lake Basin in Northwestern China. The species has an important local medicinal value, and in this study, we evaluated the chemical profile of its stems, leaves and seeds using mass spectrometry. Dried samples of stems, leaves and seeds were grinded, weighted, and used for a series of extractions with an ultrasonic device at room temperature. The chemical profiles for each tissue were determined using Gas Chromatography-Mass Spectrometry (GC-MS) and Liquid ChromatographyMass Spectrometry (LC-MS). Twenty-seven amino acids and organic… More >

  • Open Access

    ARTICLE

    Endophytic fungi from Camellia sinensis show an antimicrobial activity against the rice blast pathogen Magnaporthe grisea

    Zhu XJ, YF Hu, X Chen, YH Wang, WP Fang, XH Li

    Phyton-International Journal of Experimental Botany, Vol.83, pp. 57-63, 2014, DOI:10.32604/phyton.2014.83.057

    Abstract The purpose of this study was to evaluate the antagonistic activity of two endophytic fungal strains, Pseudocercospora kaki and Penicillium sclerotiorum, isolated from the leaves of Camellia sinensis, against the rice blast pathogen Magnaporthe grisea. The inhibitory activity of the two endophytes against M. grisea in dual-culture was compared with that in monoculture. It was confirmed that the broth and its ethyl acetate extract of the dual-culture had a much stronger inhibition activity against M. grisea than the monocultures of P. kaki and P. sclerotiorum. The antagonism index of the broth and ethyl acetate extract from dual-culture to the mycelial growth of M. griseaMore >

  • Open Access

    ARTICLE

    Analysis of volatile compounds in three unifloral native Chilean honeys

    Montenegro G1,*, M Gómez1, G Casaubon2, A Belancic2, AM Mujica1, RC Peña1

    Phyton-International Journal of Experimental Botany, Vol.78, pp. 61-65, 2009, DOI:10.32604/phyton.2009.78.067

    Abstract Three unifloral honeys were identified by the pronounced presence of specific compounds by means of sensorial analysis and SPME-GC-MS. Smoky and resinous (‘propolis’) odors characterized unifloral “quillay” (Quillaja saponaria) honey. “Corontillo” (Escallonia pulverulenta) honey was characterized by caramel and vanillin aromas, and “ulmo” (Eucryphia cordifolia) honey by having an anise scent with a floral jasmine note. Safranal was a useful marker for “corontillo” honey. Isophorone and cetoisophorone were the distinctive compounds of unifloral “ulmo” honey. In “quillay” honeys, megastigmatrienone, 2-p-hydroxyphenylalcohol and minor quantities of β-pinene and linalool oxide were correlated with their sensory properties such as resinous. More >

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