Valdés Oyervides FJ¹, C Rivas Morales², A Benavides Mendoza¹, MA Núñez González², J Verde Star², A Oranday Cárdenas², V Robledo Torres²

Phyton-International Journal of Experimental Botany, Vol.81, pp. 191-198, 2012, DOI:10.32604/phyton.2012.81.191

Abstract Four dynamic conditions to evaluate the effects of stress on production of biomass, essential oils, Thymol, carvacrol and proline accumulation in Lippia graveolens H.B.K. were investigated under greenhouse conditions. A bifactorial design assessed four environmental conditions (factor B): commercial nutritive solution (B0); Water (B1); moderate stress (B2), and intermediate (B3) stress nested under (Factor A) salinity (NaCl) and ion concentration (Cu²⁺) and (Fe²⁺) stresses. Average production of fresh weight and number of leaves were reduced under NaCl and both ion concentration conditions. However, there were no significant differences on average for the four environments. The amounts of essential oils were… More >

Guan Z-J^1,2,*, B Guo^1,*, Y-L Huo³, J-K Dai⁴, Y-H Wei¹

Phyton-International Journal of Experimental Botany, Vol.81, pp. 51-58, 2012, DOI:10.32604/phyton.2012.81.051

Abstract The initiation and development of organogenic buds and somatic embryos in HBsAg-transgenic cherry tomato (Lycopersicum esculentum var. cerasiforme) mutant were studied histologically. The leaf explants of the mutant were cultured on Murashige & Skoog (MS) basal medium supplemented with 6-BA 1.0 mg/L and IAA 0.05 mg/L for callus induction. Histological studies on the leaf explants of the mutant at various developmental stages revealed that organogenic buds first appeared in the axillary position of explants on the 14th cultured day, and then somatic embryos formed in the same mutant explants after 35 days of culture. Transmission electron microscopy and scanning electron… More >

Argüello JA¹, SB Núñez¹, V Davidenco¹, DA Suárez², L Seisdedos¹, MC Baigorria¹, N La Porta¹, G Ruiz¹, V Yossen¹

Phyton-International Journal of Experimental Botany, Vol.81, pp. 23-34, 2012, DOI:10.32604/phyton.2012.81.023

Abstract The aim of the present review was to analyze and identify the problems associated with the Production System and Chain Value of Oregano in the area of Traslasierra Valley, province of Córdoba. Traditional ecotypes, such as Criollo, Chileno II and Compacto, are cultivated in the region, as well as new ecotypes such as “Serrano Cordobés”, “Flor Rosa” and “Rosa Fuerte”. The Traslasierra Valley of Córdoba is a very suitable area for the production and for increasing the production of oregano. However, the agricultural management of the different oregano ecotypes should be optimized. Ecophysiological studies conducted to date in the region… More >

O’Connor-Sánchez¹ A, AV Domínguez-May¹, MA Keb-Llanes¹, YJ Peña-Ramírez², VA Herrera-Valencia¹

Phyton-International Journal of Experimental Botany, Vol.79, pp. 25-29, 2010, DOI:10.32604/phyton.2010.79.025

Abstract A reliable protocol for plant regeneration of Mullein Nightshade (Solanum donianum Walp.) was developed from in vitro leaf explants. They were cultured on Murashige and Skoog semisolid medium, supplemented with several combinations of zeatin riboside and 1-naphthaleneacetic acid for shoot regeneration. The formulation that originated most shoots per explant was 8.5 μM zeatin riboside in absence of 1-naphthalene acetic acid. Explants with shoots were transferred to Murashige and Skoog medium, with half the normal salt concentration and without plant growth regulators for elongation. Elongated shoots were individualized, and they rooted readily in half-strength Murashige and Skoog medium without plant growth… More >

Hernández-León R, I Velázquez-Sepúlveda, MC Orozco-Mosqueda, G Santoyo

Phyton-International Journal of Experimental Botany, Vol.79, pp. 133-139, 2010, DOI:10.32604/phyton.2010.79.133

Abstract Soil is a complex system that includes a great number and diversity of microorganisms. Until recently, only a small percentage of the bioma was known and could be studied. Currently, it is possible to have a deeper knowledge of all that unknown genomic material with the development of new tools, like metagenomics. New molecules have been discovered with various biotechnological applications, and knowledge of the diverse microbiological interactions in several environments, some of them with extreme life conditions, is much higher. We analyze the most recent literature in the field of metagenomics in this study, especially that related with the… More >

Lovey¹ RJ, P Perissé², C V ieyra³, JC Coraglio³

Phyton-International Journal of Experimental Botany, Vol.79, pp. 5-10, 2010, DOI:10.32604/phyton.2010.79.005

Abstract Cologania broussonetii (Balb.) DC. (Fabaceae) grows from the Bolivian eastern Andes to the north west of Argentina, Cordoba hills and San Luis Province. It is recognized as a native forage. The objectives of this study were to characterize the seed, the germination and the seedling of C. broussonetii. Seed structure was characterized through free hand sections and analyzed with optical and stereoscopic microscopy. The germination assay treatments were: (1) intact non-scarfied seeds at 25 °C constant, (2) scarified seeds at 25 °C constant, (3) intact non-scarified seeds at 20-30 °C alternating, and (4) scarified seeds at 20-30 °C alternating. Results… More >

Torres¹ YA, MA Long², SM Zalba¹

Phyton-International Journal of Experimental Botany, Vol.77, pp. 225-240, 2008, DOI:10.32604/phyton.2008.77.225

Abstract The objective of this work is to contribute to the biology of Pavonia cymbalaria A. St. Hil & Naudin (Malvaceae), a native species of the Southern mountains of Buenos Aires Province. This knowledge will contribute to (1) promote its use as an ornamental species, (2) its conservation and (3) stimulate the interest and public valuation of mountain systems biodiversity. Seven populations of P. cymbalaria were selected. Two of them were growing under Pinus halepensis forests to characterize different environments were the herbaceous species is growing in the wild. Environmental characteristics immediately associated to six individuals of the species were studied… More >

Ojeda-Zacarías¹ Ma del Carmen, Hugo A Luna-Olvera², Lilia H Morales-Ramos², María J Verde-Star², Teresa E Torres-Cepeda², Benito Pereyra-Alférez², Leobardo Iracheta-Donjuan³, Emilio Olivares-Sáenz⁴, Raúl Salazar-Sáenz⁴, Elizabeth Cárdenas-Cerda⁴

Phyton-International Journal of Experimental Botany, Vol.75, pp. 109-113, 2006, DOI:10.32604/phyton.2006.75.109

Abstract Pinus maximartinezii (Rzedowski) is an endemic endangered species, confined to a single population of approximately 2000 to 2500 mature trees. It covers about 400 ha in southern Zacatecas, Mexico. The success of tissue culture techniques for germplasm preservation depends on regeneration of cultures. The objective of this study was to achieve an in vitro proliferation protocol using organogenesis technique for Pinus maximartinezii. Mature seeds were surface sterilized in 6% H2O2 v/v. Isolated cotyledons and zygotic embryos were cultured on shoot induction media. DCR and GD media were supplemented with 0.3 and 0.5 mgl-1 BAP; 0.01 mgl-1 ANA and vitamin solution.… More >

Hernández-Castillo¹ FD, Adalberto Aguirre-Aguirre¹, RH Lira-Saldivar², E Guerrero-Rodríguez¹, G Gallegos-Morales¹

Phyton-International Journal of Experimental Botany, Vol.75, pp. 91-101, 2006, DOI:10.32604/phyton.2006.75.091

Abstract The biological efficiency of five stocks of Bacillus subtilis, of the mixture of the bioproducts quitosan and Larrea tridentata extract, and five synthetic fungicides were evaluated against the fungus Alternaria dauci Kühn in laboratory and greenhouse experiments. The effect of these compounds on growth and yield of carrot was also determined. Stocks of the bacteria Bacillus (B3, B9, B15 and a combination of these) and the mixture quitosan- Larrea stimulated carrot development because it reached greater width, length and weight in comparison to the control and the other treatments. Stock B3 was the best in promoting carrot growth because it… More >

Semerdjieva IB¹, EP Yankova-Tsvetkova²

Phyton-International Journal of Experimental Botany, Vol.86, pp. 318-324, 2017, DOI:10.32604/phyton.2017.86.318

Abstract Scanning electron microscope (SEM) study of the pollen and seed morphology of Peganum harmala L. and Zygophyllum fabago L. (Zygophyllaceae), in native Bulgarian populations, was carried out. Additionally, a light microscope (LM) investigation on pollen grains was made in order to elucidate the peculiarities of the major parameters of pollen surface and size. It was established that pollen grains in Z. fabago are pantoporate. Exine ornamentation is reticulate. The pollen grains of P. harmala are oblate, spheroidal, elongated, oval in shape, colporate, exine striato-rugulate. The seeds of P. harmala are oblong-oval and slightly flattened at the side (incorrect tetrahedral to… More >