WEITAO ZHENG¹, DONG JIANG², SONGEN CHEN¹, MEILING WU¹, BAOQI YAN², JIAHUI ZHAI², YUNQIANG SHI², BIN XIE¹, XINGWANG XIE², KANGHONG HU^1,*, WENXUE MA^3,*

Oncology Research, Vol.32, No.12, pp. 1837-1850, 2024, DOI:10.32604/or.2024.056565 - 13 November 2024

Abstract Objectives: The Kirsten rat sarcoma virus (KRAS) G12D oncogenic mutation poses a significant challenge in treating solid tumors due to the lack of specific and effective therapeutic interventions. This study aims to explore innovative approaches in T cell receptor (TCR) engineering and characterization to target the KRAS G12D_7-16 mutation, providing potential strategies for overcoming this therapeutic challenge. Methods: In this innovative study, we engineered and characterized two T cell receptors (TCRs), KDA11-01 and KDA11-02 with high affinity for the KRAS G12D_7-16 mutation. These TCRs were isolated from tumor-infiltrating lymphocytes (TILs) derived from tumor tissues of patients More >

Saad Akbar^1,2, Humera Azam¹, Sulaiman Sulmi Almutairi^3,*, Omar Alqahtani⁴, Habib Shah⁴, Aliya Aleryani⁴

CMC-Computers, Materials & Continua, Vol.80, No.1, pp. 1075-1104, 2024, DOI:10.32604/cmc.2024.050913 - 18 July 2024

Abstract The prompt spread of COVID-19 has emphasized the necessity for effective and precise diagnostic tools. In this article, a hybrid approach in terms of datasets as well as the methodology by utilizing a previously unexplored dataset obtained from a private hospital for detecting COVID-19, pneumonia, and normal conditions in chest X-ray images (CXIs) is proposed coupled with Explainable Artificial Intelligence (XAI). Our study leverages less preprocessing with pre-trained cutting-edge models like InceptionV3, VGG16, and VGG19 that excel in the task of feature extraction. The methodology is further enhanced by the inclusion of the t-SNE (t-Distributed… More >

Awais Khan¹, Chomyong Kim², Jung-Yeon Kim², Ahsan Aziz¹, Yunyoung Nam^3,*

CMES-Computer Modeling in Engineering & Sciences, Vol.140, No.2, pp. 1729-1755, 2024, DOI:10.32604/cmes.2024.049618 - 20 May 2024

Abstract Sleep posture surveillance is crucial for patient comfort, yet current systems face difficulties in providing comprehensive studies due to the obstruction caused by blankets. Precise posture assessment remains challenging because of the complex nature of the human body and variations in sleep patterns. Consequently, this study introduces an innovative method utilizing RGB and thermal cameras for comprehensive posture classification, thereby enhancing the analysis of body position and comfort. This method begins by capturing a dataset of sleep postures in the form of videos using RGB and thermal cameras, which depict six commonly adopted postures: supine,… More > Graphic Abstract

Imad Saud Al Naimi^1,2,*, Syed Alwee Aljunid Syed Junid¹, Muhammad lmran Ahmad^1,*, K. Suresh Manic^2,3

CMC-Computers, Materials & Continua, Vol.79, No.2, pp. 2585-2608, 2024, DOI:10.32604/cmc.2024.046461 - 15 May 2024

Abstract Augmentation of abnormal cells in the brain causes brain tumor (BT), and early screening and treatment will reduce its harshness in patients. BT’s clinical level screening is usually performed with Magnetic Resonance Imaging (MRI) due to its multi-modality nature. The overall aims of the study is to introduce, test and verify an advanced image processing technique with algorithms to automatically extract tumour sections from brain MRI scans, facilitating improved accuracy. The research intends to devise a reliable framework for detecting the BT region in the two-dimensional (2D) MRI slice, and identifying its class with improved… More >

JIYU WANG^1,#, LIUYING YI^2,#, KEKE HUANG¹, YANGYANG WANG¹, HUIPING WANG¹, ZHIMIN ZHAI^1,*

BIOCELL, Vol.48, No.3, pp. 453-462, 2024, DOI:10.32604/biocell.2024.047871 - 15 March 2024

Abstract Background: The primary cause of treatment failure in patients with refractory or relapsed B-cell non-Hodgkin lymphoma (r/r B-NHL) is resistance to current therapies, and therapy-induced senescence (TIS) stands out as a crucial mechanism contributing to tumor drug resistance. Here, we analyzed SENEX/Rho GTPase Activating Protein 18 (ARHGAP18) expression and prognostic significance in doxorubicin-induced B-NHL-TIS model and r/r B-NHL patients, investigating its target in B-NHL cell senescence and the effect of combining specific inhibitors on apoptosis resistance in B-NHL-TIS cells. Methods: Raji cells were transfected with the human SENEX shRNA recombinant lentiviral vector (Sh-SENEX) and the empty vector… More >

Heba M. El-Hoseny^1,*, Heba F. Elsepae², Wael A. Mohamed², Ayman S. Selmy²

CMC-Computers, Materials & Continua, Vol.77, No.2, pp. 1855-1872, 2023, DOI:10.32604/cmc.2023.042107 - 29 November 2023

Abstract Diabetic retinopathy is a critical eye condition that, if not treated, can lead to vision loss. Traditional methods of diagnosing and treating the disease are time-consuming and expensive. However, machine learning and deep transfer learning (DTL) techniques have shown promise in medical applications, including detecting, classifying, and segmenting diabetic retinopathy. These advanced techniques offer higher accuracy and performance. Computer-Aided Diagnosis (CAD) is crucial in speeding up classification and providing accurate disease diagnoses. Overall, these technological advancements hold great potential for improving the management of diabetic retinopathy. The study’s objective was to differentiate between different classes… More >

Lei Wang¹, Runying Wang¹, Yi Li³, Yichen Zhao^1,*, Degang Zhao^1,2,*

Phyton-International Journal of Experimental Botany, Vol.92, No.11, pp. 3063-3075, 2023, DOI:10.32604/phyton.2023.043803 - 24 October 2023

Abstract Adventitious root formation poses a major constraint on the tissue culture and genetic transformation of Eucommia ulmoides. Micrografting offers a new method for the transplantation of genetic transformation, and its success depends on the formation of graft unions. This study used transgenic rootless test-tube seedlings as scions and seedlings from seed as rootstocks during micrografting to avoid the rooting issues that occur during tissue culture and to investigate the role of the EuEG1 gene in the graft healing process. We found that the EuEG1 gene is a vital regulator of graft, and its overexpression contributes to the… More >

LONGSHENG FU¹, JINFANG HU¹, FENG SHAO², YAOQI WU¹, WEI BAI³, MINGJIN JIANG³, HAO CHEN⁴, LIHUA CHEN², YANNI LV^1,*

BIOCELL, Vol.47, No.8, pp. 1869-1883, 2023, DOI:10.32604/biocell.2023.028602 - 28 August 2023

Abstract Background: Biochanin A is an excellent dietary isoflavone that has the concomitant function of both medicine and foodstuff. The attenuation function of biochanin A on blood-brain barrier (BBB) damage induced by cerebral ischemia-reperfusion remains unclear. Methods: C57BL/6 mice were subjected to 1 h middle cerebral artery occlusion (MCAO) followed by 24 h reperfusion. The infarct volume of the brain was stained by TTC, while leakage of the brain was quantitatively stained by Evans blue, and the neurologic deficit score was measured. Microglial-induced morphologic changes were observed via immunofluorescence staining, and rolling and adhering leukocytes in… More >

Jinlan Xu^*, Shuxin Xiao, Gang Xu, Renshu Gu

CMES-Computer Modeling in Engineering & Sciences, Vol.137, No.2, pp. 1957-1973, 2023, DOI:10.32604/cmes.2023.028665 - 26 June 2023

Abstract In this paper, we propose a parameterization transfer algorithm for planar domains bounded by B-spline curves, where the shapes of the planar domains are similar. The domain geometries are considered to be similar if their simplified skeletons have the same structures. One domain we call source domain, and it is parameterized using multi-patch B-spline surfaces. The resulting parameterization is C1 continuous in the regular region and G1 continuous around singular points regardless of whether the parameterization of the source domain is C1/G1 continuous or not. In this algorithm, boundary control points of the source domain… More >

YUE GUAN^1,#, TINGTING ZHANG^2,#, JIANAN YU³, JIAWEI LIU⁴, WENYUAN LI⁵, YUJIA ZHENG⁶, JIALE WANG³, YUE LIU³, FENGGUO ZHAI^2,7,*

BIOCELL, Vol.47, No.7, pp. 1583-1594, 2023, DOI:10.32604/biocell.2023.028016 - 21 June 2023

Abstract Background: A preliminary miRNA screening showed that expression levels of rno-miRNA-27a-3p were significantly increased in the serum and brain tissues of rats undergoing cerebral ischemia. In recent years, there is evidence of the protective capacity of the saponins extracted from panax ginseng and its primary active ingredient ginsenosideRg1oncerebral ischemic injury. Methods: Fetal rat neurons (FRNs) were cultured in glucose-and-serum-free medium and exposed to hypoxia to establish a cerebral ischemia model in vitro (oxygen and glucose deprivation model, OGD). Antioxidant indexes (CAT, SOD), inflammatory markers (MPO, TNF-α and IL-6), and the expression of apoptosis and proliferation associated… More >