Sanae Haga*, Akira Kanno†, Takeaki Ozawa‡, Naoki Morita§, Mami Asano*¶,¶ and Michitaka Ozaki*¶
Oncology Research, Vol.26, No.3, pp. 503-513, 2018, DOI:10.3727/096504017X15005102445191
Abstract Liver injury is often observed in various pathological conditions including posthepatectomy state and
cancer chemotherapy. It occurs mainly as a consequence of the combined necrotic and apoptotic types of cell
death. In order to study liver/hepatocyte injury by the necrotic type of cell death, we studied signal-regulated
necrosis (necroptosis) by developing a new optic probe for detecting receptor-interacting protein kinase 1
(RIP)/RIP3 binding, an essential process for necroptosis induction. In the mouse hepatocyte cell line, TIB-73
cells, TNF-a/cycloheximide (T/C) induced RIP1/3 binding only when caspase activity was suppressed by
the caspase-specific inhibitor z-VAD-fmk (zVAD). T/C/zVAD-induced… More >
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